Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4949 and PP_4950 overlap by 1 nucleotidesPP_4950 and PP_4951 are separated by 109 nucleotidesPP_4951 and PP_4952 are separated by 64 nucleotidesPP_4952 and PP_4953 are separated by 122 nucleotides PP_4949: PP_4949 - putative peptidase, TldD/PmbA family, at 5,637,467 to 5,638,909 _4949 PP_4950: PP_4950 - conserved protein of unknown function, at 5,638,909 to 5,640,228 _4950 PP_4951: PP_4951 - Drug resistance transporter, EmrB/QacA family, at 5,640,338 to 5,641,765 _4951 PP_4952: PP_4952 - ATP-dependent RNA helicase, specific for 23S rRNA, at 5,641,830 to 5,643,215 _4952 PP_4953: PP_4953 - putative oxidoreductase with FAD/NAD(P)-binding domain, at 5,643,338 to 5,644,519 _4953
Group Condition PP_4949 PP_4950 PP_4951 PP_4952 PP_4953
phage PUT8 MOI 0.1 -0.0 +0.1 +0.2 -5.1 +0.3
nitrogen source NAG (N); with MOPS -0.1 +0.2 +0.0 -4.1 -0.5
pH Growth at pH6 and (C) D-Glucose -0.4 -0.0 +0.4 -4.2 +0.4
nitrogen source nitrate (N) +0.2 -0.1 +0.4 -4.1 -0.3
carbon source Trisodium citrate (C) +0.1 +0.1 -0.0 -4.2 +0.2
nitrogen source 2,6-diaminopimelic (N) -0.3 -0.2 -0.4 -2.3 -0.4
nitrogen source D-2-Aminobutyric (N) +0.1 -0.1 -0.3 -2.8 -0.1
carbon source 2-Keto-D-gluconic hemicalcium salt (C) +0.1 +0.0 +0.2 -3.2 -0.1
phage P. putida Sci small MOI 0.1 -0.4 -0.7 -0.1 -0.4 -1.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.4 +0.3 -0.6 -2.6 -0.2
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.0 -0.1 +0.5 -2.5 -0.5
carbon source L-Leucine (C) +0.1 -0.0 +0.1 -2.3 -0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs +0.1 +0.3 -0.0 -2.7 -0.0
phage P. putida Sci Big MOI 0.1 -0.1 -0.4 +0.3 -2.0 +0.0
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.3 +0.1 -0.1 -3.0 +0.5
phage P. putida PUT10 MOI 10 +0.1 +0.3 -0.2 -2.1 -0.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.6 +0.5 +0.1 -1.6 -0.3
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.2 +0.0 +0.3 -2.2 +0.1
temperature shift Temperature shift 30_to_10; with MOPS -0.3 -0.0 +0.1 -2.0 +0.4
carbon source Glutaric (C) +0.5 -0.1 +0.1 -2.2 +0.1
nitrogen source Ammonium chloride (N) +0.3 -0.1 +0.1 -2.3 +0.4
carbon source 3-Hydroxydecanoic (C) -0.3 +0.1 +0.1 -1.9 +0.5
carbon source Valerolactone (C) +0.7 +0.2 +0.0 -2.3 -0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.2 +0.2 +0.5 -2.8 +0.5
carbon source Valerolactone (C) +0.1 +0.1 +0.1 -2.2 +0.7
carbon source D-Glucose (C) -0.1 +0.1 +0.3 +0.6 -1.8
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.3 +0.2 +0.7 -1.2 +0.5
phage P. putida Sci small MOI 0.1 -0.3 +0.1 -0.4 +1.6 +0.7
nitrogen source NAG (N); with MOPS -0.2 +0.6 +0.6 +1.3 +0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 -0.1 +1.7 +1.7 -0.9
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