Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4915 and PP_4916 are separated by 35 nucleotidesPP_4916 and PP_4917 are separated by 131 nucleotidesPP_4917 and PP_4918 are separated by 85 nucleotidesPP_4918 and PP_4919 overlap by 10 nucleotides PP_4915: PP_4915 - DNA topoisomerase IV subunit B, at 5,587,243 to 5,589,147 _4915 PP_4916: PP_4916 - putative esterase, at 5,589,183 to 5,589,791 _4916 PP_4917: PP_4917 - 3',5'-cyclic-nucleotide phosphodiesterase, at 5,589,923 to 5,590,723 _4917 PP_4918: PP_4918 - conserved protein of unknown function, at 5,590,809 to 5,591,261 _4918 PP_4919: PP_4919 - ADP-ribose/sugar pyrophosphatase, at 5,591,252 to 5,591,881 _4919
Group Condition PP_4915 PP_4916 PP_4917 PP_4918 PP_4919
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs N.D. -0.7 -4.5 -0.0 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs N.D. -0.7 -2.6 -0.8 -0.4
carbon source 3-methyl-1-butanol 0.1 vol% (C) N.D. -0.6 -4.0 +0.1 +0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs N.D. -0.5 -3.2 -0.2 -0.2
carbon source carbon source Bisabolene 48.9 mM N.D. -0.8 -3.7 +0.5 -0.1
carbon source D-Glucose (C) N.D. -0.1 -3.7 +0.5 -0.4
carbon source Tween 20 (C) N.D. -1.2 -2.2 +0.1 -0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs N.D. -1.2 -2.4 -0.1 +0.3
phage moi 100 Time6-phageFRS N.D. +0.1 -3.4 -0.0 +0.1
no phage control no phage control N.D. +0.2 -3.2 +0.0 -0.2
carbon source Cholinium lysinate (C) N.D. +0.5 -3.6 +0.0 +0.0
no phage control no phage control N.D. +0.3 -2.8 -0.1 -0.3
phagefrs Time6-FRS N.D. -0.3 -3.1 +0.2 +0.2
no phage control no phage control N.D. +0.3 -3.3 +0.1 +0.0
phage FRSphage N.D. +0.3 -3.1 -0.0 -0.1
pH pH 5 N.D. +0.3 -4.0 +0.5 +0.3
phagefrs Time6-FRS N.D. -0.4 -2.6 -0.1 +0.3
carbon source carbon source 3-O-methyl-glucose 10 mM N.D. -1.0 -2.2 +0.3 +0.2
carbon source carbon source Bisabolol 45 mM N.D. -0.1 -3.2 +0.5 +0.1
carbon source carbon source D-Glucose 55.5 mM N.D. +0.2 -3.6 +0.5 +0.3
pH pH 4 N.D. -0.6 -2.9 +0.7 +0.2
carbon source carbon source Glycerol 108.6 mM N.D. +0.4 -3.7 +0.6 +0.2
carbon source carbon source 4-vinylphenol 0.01 mM N.D. -0.5 -2.8 +0.3 +0.5
pH pH 9 N.D. +0.2 -3.3 +0.5 +0.1
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X N.D. +0.3 -3.1 +0.2 +0.2
carbon source carbon source 2-Deoxy-D-glucose 10 mM N.D. -0.0 -2.7 +0.5 +0.1
pH Growth at pH6 and (C) Trisodium citrate N.D. +0.3 -2.7 +0.2 +0.2
carbon source carbon source 2-Deoxy-D-glucose 100 mM N.D. +0.0 -2.7 +0.8 +0.2
micoeukaryotes Tetrahymena sp., Mixed culture-1 N.D. +0.8 -2.2 +0.1 -0.4
micoeukaryotes Tetrahymena sp., Mixed culture-2 N.D. +1.4 -1.7 +0.2 -0.2
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