Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4898 and PP_4899 overlap by 13 nucleotidesPP_4899 and PP_4900 are separated by 65 nucleotidesPP_4900 and PP_4901 are separated by 140 nucleotidesPP_4901 and PP_4902 are separated by 91 nucleotides PP_4898: PP_4898 - ATPase, at 5,568,927 to 5,569,400 _4898 PP_4899: PP_4899 - ADP-dependent (S)-NAD(P)H-hydrate dehydratase, at 5,569,388 to 5,570,248 _4899 PP_4900: PP_4900 - epoxyqueuosine reductase, at 5,570,314 to 5,571,378 _4900 PP_4901: PP_4901 - conserved inner membrane protein, at 5,571,519 to 5,572,130 _4901 PP_4902: PP_4902 - Oligoribonuclease, at 5,572,222 to 5,572,764 _4902
Group Condition PP_4898 PP_4899 PP_4900 PP_4901 PP_4902
stress R2A with Fusaric 480 ug/mL N.D. +0.3 -0.5 +0.2 -7.6
micoeukaryotes C.elegans, mixed culture-2 N.D. -0.0 -1.4 -0.2 -5.9
micoeukaryotes Tetrahymena sp., Mixed culture-2 N.D. -0.6 -0.8 +0.1 -5.8
micoeukaryotes Tetrahymena sp., Mixed culture-1 N.D. +0.0 -0.8 -0.1 -6.2
nitrogen source nitrogen source nitrite 0.5mM N.D. -0.5 +0.1 +0.2 -5.6
micoeukaryotes C.elegans, mixed culture-1 N.D. -0.3 -1.3 -0.2 -3.8
carbon source 3-methyl-1-butanol 0.1 vol% (C) N.D. +0.2 -1.8 -0.4 -3.7
r2a control with 0.2x vogels R2A control with 0.2X Vogels N.D. +0.5 +0.1 -0.4 -5.8
stress R2A with Fusaric 480 ug/mL N.D. -0.0 -0.3 +0.1 -5.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs N.D. +0.1 -0.1 +0.1 -5.5
carbon source M9 minimal media_noCarbon with Trisodium citrate 10 mM; 30C; then outgrowth in LB N.D. -0.0 -0.1 -0.0 -5.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs N.D. +0.3 -0.1 +0.2 -5.7
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs N.D. -0.0 -0.4 +0.3 -5.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs N.D. +0.2 -0.3 +0.2 -5.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs N.D. +0.2 -0.4 -0.0 -4.8
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs N.D. -0.1 -0.2 +0.3 -5.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs N.D. +0.0 -0.4 +0.2 -4.9
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs N.D. +0.2 -0.1 +0.1 -5.0
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X N.D. -0.3 +0.5 +0.0 -5.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs N.D. +0.1 -0.4 +0.4 -4.8
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X N.D. -0.2 +0.5 -0.1 -4.9
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs N.D. +0.2 -0.6 +0.4 -4.7
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs N.D. +0.5 -0.9 +0.8 -4.9
pH Growth at pH9 and (C) Trisodium citrate N.D. +0.4 +0.0 -0.7 -4.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs N.D. +0.1 -0.3 +0.5 -4.7
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs N.D. -0.0 -0.2 +0.7 -4.9
biofilm mediium pdms biofilm mediium PDMS; 7 hours N.D. +0.2 +0.1 +0.4 -4.8
phage P. put South MOI 10 N.D. +0.3 +0.4 +0.2 -4.9
phage PUT8 MOI 0.1 N.D. +0.5 +0.6 -0.2 -4.4
nitrogen source DL-2-Aminobutyric (N) N.D. +0.4 -0.9 +0.0 +4.1
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