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Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_4898 and PP_4899 overlap by 13 nucleotides
PP_4899 and PP_4900 are separated by 65 nucleotides
PP_4900 and PP_4901 are separated by 140 nucleotides
PP_4901 and PP_4902 are separated by 91 nucleotides
PP_4898: PP_4898 - ATPase, at 5,568,927 to 5,569,400
_4898
PP_4899: PP_4899 - ADP-dependent (S)-NAD(P)H-hydrate dehydratase, at 5,569,388 to 5,570,248
_4899
PP_4900: PP_4900 - epoxyqueuosine reductase, at 5,570,314 to 5,571,378
_4900
PP_4901: PP_4901 - conserved inner membrane protein, at 5,571,519 to 5,572,130
_4901
PP_4902: PP_4902 - Oligoribonuclease, at 5,572,222 to 5,572,764
_4902
Group
Condition
PP
_4898
PP
_4899
PP
_4900
PP
_4901
PP
_4902
stress
R2A with Fusaric 480 ug/mL
N.D.
+0.3
-0.5
+0.2
-7.6
micoeukaryotes
C.elegans, mixed culture-2
N.D.
-0.0
-1.4
-0.2
-5.9
micoeukaryotes
Tetrahymena sp., Mixed culture-2
N.D.
-0.6
-0.8
+0.1
-5.8
micoeukaryotes
Tetrahymena sp., Mixed culture-1
N.D.
+0.0
-0.8
-0.1
-6.2
nitrogen source
nitrogen source nitrite 0.5mM
N.D.
-0.5
+0.1
+0.2
-5.6
micoeukaryotes
C.elegans, mixed culture-1
N.D.
-0.3
-1.3
-0.2
-3.8
carbon source
3-methyl-1-butanol 0.1 vol% (C)
N.D.
+0.2
-1.8
-0.4
-3.7
r2a control with 0.2x vogels
R2A control with 0.2X Vogels
N.D.
+0.5
+0.1
-0.4
-5.8
stress
R2A with Fusaric 480 ug/mL
N.D.
-0.0
-0.3
+0.1
-5.4
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
N.D.
+0.1
-0.1
+0.1
-5.5
carbon source
M9 minimal media_noCarbon with Trisodium citrate 10 mM; 30C; then outgrowth in LB
N.D.
-0.0
-0.1
-0.0
-5.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
N.D.
+0.3
-0.1
+0.2
-5.7
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
N.D.
-0.0
-0.4
+0.3
-5.2
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
N.D.
+0.2
-0.3
+0.2
-5.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
N.D.
+0.2
-0.4
-0.0
-4.8
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
N.D.
-0.1
-0.2
+0.3
-5.0
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
N.D.
+0.0
-0.4
+0.2
-4.9
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs
N.D.
+0.2
-0.1
+0.1
-5.0
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
N.D.
-0.3
+0.5
+0.0
-5.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
N.D.
+0.1
-0.4
+0.4
-4.8
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
N.D.
-0.2
+0.5
-0.1
-4.9
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
N.D.
+0.2
-0.6
+0.4
-4.7
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
N.D.
+0.5
-0.9
+0.8
-4.9
pH
Growth at pH9 and (C) Trisodium citrate
N.D.
+0.4
+0.0
-0.7
-4.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
N.D.
+0.1
-0.3
+0.5
-4.7
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
N.D.
-0.0
-0.2
+0.7
-4.9
biofilm mediium pdms
biofilm mediium PDMS; 7 hours
N.D.
+0.2
+0.1
+0.4
-4.8
phage
P. put South MOI 10
N.D.
+0.3
+0.4
+0.2
-4.9
phage
PUT8 MOI 0.1
N.D.
+0.5
+0.6
-0.2
-4.4
nitrogen source
DL-2-Aminobutyric (N)
N.D.
+0.4
-0.9
+0.0
+4.1
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