Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4838 and PP_4839 are separated by 56 nucleotidesPP_4839 and PP_4840 are separated by 198 nucleotidesPP_4840 and PP_4841 are separated by 249 nucleotidesPP_4841 and PP_4842 are separated by 140 nucleotides PP_4838: PP_4838 - Outer membrane copper receptor OprC, at 5,503,051 to 5,505,117 _4838 PP_4839: PP_4839 - putative iron-regulated membrane protein, at 5,505,174 to 5,506,541 _4839 PP_4840: PP_4840 - D-alanine, beta-alanine, D-serine, glycine permease, at 5,506,740 to 5,508,146 _4840 PP_4841: PP_4841 - putative Urea ABC transporter, periplasmic protein, at 5,508,396 to 5,509,661 _4841 PP_4842: PP_4842 - putative Urea ABC transporter, at 5,509,802 to 5,511,289 _4842
Group Condition PP_4838 PP_4839 PP_4840 PP_4841 PP_4842
phage P. putida Sci Big MOI 0.1 -0.2 -0.3 -0.7 -0.7 -0.0
phage P. putida Sci small MOI 0.1 -0.5 -0.5 -0.0 -0.7 -0.1
carbon source Delta-Nonalactone (C) -0.0 +0.0 -1.5 -0.1 -0.1
reactor 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -0.8 -0.2 +0.0 -0.2 -0.3
reactor 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. -0.7 -0.4 -0.0 -0.1 -0.3
carbon source Delta-Decalactone (C) -0.2 -0.1 -0.8 -0.2 +0.1
phage P. putida Sci small MOI 0.1 -0.6 +0.0 -0.7 +0.1 +0.2
nitrogen source 2,6-diaminopimelic (N) -0.2 +0.2 +0.0 -0.8 -0.1
carbon source Myristic (C) -0.2 +0.1 -0.6 -0.4 +0.4
stress R2A with Fusaric 480 ug/mL -0.2 -1.0 +0.2 +0.1 +0.3
nitrogen source Propandiamine (N) +0.3 +0.0 +0.2 -0.8 -0.4
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -1.1 +0.1 +0.1 +0.2 +0.1
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -1.1 +0.1 +0.3 +0.1 +0.2
pH Growth at pH7 and (C) Trisodium citrate +0.1 +0.2 +0.3 -0.0 -0.8
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.6 -0.5 -0.0 +0.3 -0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs +0.4 +0.2 -0.0 +0.3 -0.5
pH pH 9 -0.2 +0.6 +0.3 +0.2 -0.2
phage PUT 8 MOI 1 +0.4 +0.5 -0.3 +0.2 -0.0
carbon source carbon source 2-Deoxy-D-glucose 50 mM -0.2 +0.6 +0.3 +0.2 -0.0
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 +0.3 -0.2 +0.3 +0.1
nitrogen source DL-2-Aminobutyric (N) +0.6 -0.0 +0.8 -0.2 -0.1
carbon source carbon source 2-Deoxy-D-glucose 100 mM -0.1 +0.4 +0.3 +0.6 +0.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +0.2 +0.3 +0.3 +0.2 +0.3
pH Growth at pH7 and (C) Trisodium citrate +0.1 +0.2 +0.3 +0.4 +0.3
nitrogen source DL-2-Aminobutyric (N) +0.7 +0.0 +1.0 -0.1 -0.2
nitrogen source Beta-alanine (N) +0.4 -0.2 +1.1 +0.0 +0.1
nitrogen source Beta-alanine (N) +0.3 -0.0 +1.1 +0.1 -0.0
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 +0.7 +0.4 +0.4 +0.2
nitrogen source D-2-Aminobutyric (N) +0.3 +0.7 +1.7 +0.0 -0.0
nitrogen source D-2-Aminobutyric (N) +0.8 +0.1 +1.5 +0.4 +0.2
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