Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4816 and PP_4817 are separated by 137 nucleotidesPP_4817 and PP_4818 are separated by 3 nucleotidesPP_4818 and PP_4819 are separated by 42 nucleotidesPP_4819 and PP_4820 are separated by 207 nucleotides PP_4816: PP_4816 - conserved protein of unknown function, at 5,478,512 to 5,478,880 _4816 PP_4817: PP_4817 - MaoC domain protein, at 5,479,018 to 5,479,473 _4817 PP_4818: PP_4818 - ribosomal protein L11 methyltransferase, at 5,479,477 to 5,480,469 _4818 PP_4819: PP_4819 - conserved protein of unknown function, at 5,480,512 to 5,481,867 _4819 PP_4820: PP_4820 - tRNA-dihydrouridine synthase B, at 5,482,075 to 5,483,088 _4820
Group Condition PP_4816 PP_4817 PP_4818 PP_4819 PP_4820
carbon source 5-Hydroxydodecanoate (C) -0.1 -5.6 -0.6 -0.2 +0.1
carbon source Delta-Undecalactone (C) +0.1 -6.5 -0.0 -0.1 +0.4
dilutelb LB 0.1x with 55.5 mM D-Glucose +0.2 -0.4 -1.9 -3.5 +0.1
carbon source Delta-Dodecalactone (C) -0.0 -5.1 -0.1 +0.4 +0.7
carbon source 5-Hydroxydodecanoate (C) +0.1 -4.7 +0.4 +0.1 +0.2
carbon source Delta-Undecalactone (C) +0.0 -3.5 -0.0 -0.1 +0.1
supernatant LB 0.1x with 55.5 mM D-Glucose with Supernatant: Acinetobacter -0.1 -0.0 -0.7 -1.5 -0.7
carbon source Delta-Dodecalactone (C) +0.0 -4.1 +0.2 +0.2 +0.8
micoeukaryotes C.elegans, mixed culture-1 +0.3 -0.6 -0.5 -1.2 -1.0
nitrogen source nitrate (N) +0.0 -0.1 -1.5 -0.7 -0.6
pH Growth at pH6 and (C) Trisodium citrate -0.1 +0.1 -1.6 -0.5 -0.7
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.8 -0.3 -0.6 +0.0 -1.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days +0.0 +0.2 +0.4 -1.5 -1.7
pH Growth at pH6 and (C) Trisodium citrate -0.3 -0.2 -1.8 -0.3 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.1 -3.0 +0.6 +0.1 +0.1
carbon source 3-Hydroxydecanoic (C) +0.5 -3.0 +0.4 -0.3 +0.1
carbon source ferulate (C); with MOPS +0.1 -1.8 -0.6 +0.2 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs +0.1 +0.8 -2.5 -0.4 +0.1
carbon source Delta-Decalactone (C) -0.2 -2.5 +0.5 +0.3 -0.0
carbon source 3-Hydroxydecanoic (C) +0.1 -2.3 +0.5 -0.2 +0.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 +0.2 -0.9 -0.4 -0.8
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -0.6 +1.1 -0.5 -0.2 -0.6
pH pH 9 +0.1 +1.0 -1.4 -0.1 -0.1
carbon source carbon source 2-Deoxy-D-glucose 10 mM +0.2 +1.1 -1.0 -0.2 -0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.7 +0.5 +1.3 +0.1 -1.4
carbon source Valerolactone (C) +0.5 -0.4 +0.8 +0.2 -1.1
carbon source Decanoic (C) +0.1 +1.6 +0.4 +0.5 -0.7
carbon source L-Leucine (C) +0.3 +1.5 -0.2 +0.1 +1.6
carbon source L-Leucine (C) +0.4 +1.2 +0.4 +0.3 +1.2
carbon source L-Leucine (C) +0.3 +1.1 +0.4 +0.4 +1.9
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