Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4790 and PP_4791 are separated by 354 nucleotidesPP_4791 and PP_4792 are separated by 160 nucleotidesPP_4792 and PP_4793 are separated by 134 nucleotidesPP_4793 and PP_4794 are separated by 227 nucleotides PP_4790: PP_4790 - Apolipoprotein N-acyltransferase, at 5,451,470 to 5,452,987 _4790 PP_4791: PP_4791 - transposase, at 5,453,342 to 5,454,670 _4791 PP_4792: PP_4792 - conserved exported protein of unknown function, at 5,454,831 to 5,455,592 _4792 PP_4793: PP_4793 - conserved protein of unknown function, at 5,455,727 to 5,456,041 _4793 PP_4794: PP_4794 - Leucine--tRNA ligase, at 5,456,269 to 5,458,875 _4794
Group Condition PP_4790 PP_4791 PP_4792 PP_4793 PP_4794
nitrogen source NAG (N); with MOPS N.D. N.D. -0.5 -5.0 N.D.
pH Growth at pH6 and (C) Trisodium citrate N.D. N.D. -0.9 -2.9 N.D.
pH Growth at pH7 and (C) Trisodium citrate N.D. N.D. +1.2 -4.9 N.D.
carbon source butyrate (C) N.D. N.D. +0.7 -3.8 N.D.
nitrogen source nitrite (N) N.D. N.D. -2.0 -1.0 N.D.
phage P. put South MOI 10 N.D. N.D. -1.8 -0.8 N.D.
pH Growth at pH7 and (C) D-Glucose N.D. N.D. -2.3 -0.3 N.D.
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. N.D. N.D. -1.6 -1.0 N.D.
nitrogen source nitrogen source nitrate 5mM N.D. N.D. -1.7 -0.8 N.D.
carbon source carbon source 4-vinylphenol 0.1 mM N.D. N.D. -1.5 -0.9 N.D.
phage P. putida Missley MOI 0.1 N.D. N.D. +0.4 -2.8 N.D.
nitrogen source D-2-Aminobutyric (N) N.D. N.D. -1.2 -1.2 N.D.
carbon source ferulate (C); with MOPS N.D. N.D. +0.6 -2.9 N.D.
pH Growth at pH7 and (C) Trisodium citrate N.D. N.D. -0.8 -1.5 N.D.
carbon source 1,3-Hexanediol (C) N.D. N.D. -0.6 -1.6 N.D.
phage P. putida South MOI 10 N.D. N.D. -1.1 -1.1 N.D.
mixed carbon source mixed (C)s Trisodium citrate 10 mM and 3-Chloro-L-tyrosine 10 mM N.D. N.D. -0.6 -1.6 N.D.
mixed carbon source mixed (C)s Trisodium citrate 10 mM and L-Meta-tyrosine 10 mM N.D. N.D. -0.9 -1.2 N.D.
temperature Growth at 25C N.D. N.D. -0.5 -1.6 N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs N.D. N.D. -1.5 -0.7 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. -2.3 +0.2 N.D.
pH Growth at pH7 and (C) D-Glucose N.D. N.D. -1.7 -0.4 N.D.
pH Growth at pH7 and (C) Trisodium citrate N.D. N.D. -1.0 -1.1 N.D.
stress R2A with Fusaric 240 ug/mL N.D. N.D. +0.1 -2.0 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. N.D. +0.4 -1.7 N.D.
carbon source Lauric (C) N.D. N.D. +0.7 -1.7 N.D.
lb LB N.D. N.D. -1.5 +0.7 N.D.
carbon source ferulate (C); with MOPS N.D. N.D. +0.7 -1.4 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days N.D. N.D. +1.1 -1.8 N.D.
carbon source D-Fructose (C) N.D. N.D. +0.8 -1.3 N.D.
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