Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_5710 and PP_4740 are separated by 386 nucleotidesPP_4740 and PP_4741 are separated by 100 nucleotidesPP_4741 and PP_4742 overlap by 4 nucleotidesPP_4742 and PP_5711 are separated by 10 nucleotides PP_5710: PP_5710 - exported protein of unknown function, at 5,392,091 to 5,392,672 _5710 PP_4740: PP_4740 - Type I restriction enzyme EcoAI R protein, at 5,393,059 to 5,395,422 _4740 PP_4741: PP_4741 - Type I restriction enzyme EcoEI M protein, at 5,395,523 to 5,396,992 _4741 PP_4742: PP_4742 - Type I restriction-modification system, S subunit, at 5,396,989 to 5,398,719 _4742 PP_5711: PP_5711 - protein of unknown function, at 5,398,730 to 5,399,158 _5711
Group Condition PP_5710 PP_4740 PP_4741 PP_4742 PP_5711
pH Growth at pH8 and (C) D-Glucose -0.8 -0.1 -0.1 -0.2 -0.9
nitrogen source Ammonium chloride (N) -0.1 +0.1 -0.3 -0.1 -1.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days -0.0 -0.2 +0.0 +0.3 -1.6
temperature shift Temperature shift 30_to_25; with MOPS -0.2 +0.1 -0.0 -0.1 -1.1
no stress control Growth with no stress -0.2 +0.0 -0.0 -0.2 -1.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.2 +0.2 +0.2 -0.5 -1.0
carbon source carbon source 4-vinylphenol 0.01 mM -0.5 +0.1 -0.2 -0.2 -0.5
pH Growth at pH7 and (C) Trisodium citrate +0.3 -0.1 +0.1 -0.6 -1.0
carbon source Myristic (C) -0.2 -0.0 +0.2 +0.2 -1.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs +0.3 -0.1 +0.0 -0.3 -1.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.3 +0.1 -0.4 -0.1 -0.8
carbon source Tween 20 (C) -0.6 -0.1 +0.3 -0.3 -0.4
carbon source D-Glucose (C); with MOPS -0.3 +0.1 -0.3 +0.1 -0.5
pH Growth at pH9 and (C) Trisodium citrate -0.5 +0.0 +0.2 +0.4 -0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.4 -0.2 -0.2 +0.2 +0.3
temperature shift Temperature shift 10_to_25; with MOPS +0.4 +0.1 -0.1 -0.1 -0.7
nitrogen source nitrate (N) +0.2 +0.1 +0.1 +0.3 -0.9
pH Growth at pH7 and (C) D-Glucose +0.3 +0.1 -0.2 +0.5 -0.8
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.0 -0.2 -0.5 +0.0 +0.7
carbon source ferulate (C); with MOPS +0.3 +0.2 -0.0 +0.3 -0.7
nitrogen source 2,6-diaminopimelic (N) +0.4 +0.4 +0.2 +0.2 -0.8
pH Growth at pH7 and (C) Trisodium citrate -0.4 +0.2 +0.1 +0.3 +0.6
carbon source Crotonic (C) -0.3 +0.0 +0.1 +0.4 +0.6
carbon source Valerolactone (C) -0.2 +0.2 +0.2 +0.1 +1.0
carbon source 4-Hydroxyvalerate (C) (40mM) +0.3 +0.2 +0.3 +0.4 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.4 +0.1 +0.1 +0.4 +0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 +0.0 +0.3 +0.5 +0.7
nitrogen source D-2-Aminobutyric (N) +0.6 +0.1 +0.3 +0.3 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.5 +0.2 -0.0 +0.3 +0.6
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.0 -0.0 +0.3 +0.1 +1.3
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