Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4701 and PP_4702 are separated by 177 nucleotidesPP_4702 and PP_4703 are separated by 41 nucleotidesPP_4703 and PP_4704 are separated by 78 nucleotidesPP_4704 and PP_4705 are separated by 81 nucleotides PP_4701: PP_4701 - glucose-6-phosphate isomerase, at 5,342,761 to 5,344,425 _4701 PP_4702: PP_4702 - acetyl-CoA synthetase, at 5,344,603 to 5,346,537 _4702 PP_4703: PP_4703 - conserved protein of unknown function, at 5,346,579 to 5,347,448 _4703 PP_4704: PP_4704 - conserved protein of unknown function, at 5,347,527 to 5,350,463 _4704 PP_4705: PP_4705 - Glyoxalase, at 5,350,545 to 5,350,958 _4705
Group Condition PP_4701 PP_4702 PP_4703 PP_4704 PP_4705
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -5.4 -0.0 +0.2 +0.1 -0.8
carbon source acetate (C) -4.6 +0.1 -0.5 -0.2 -0.1
temperature shift Temperature shift 30_to_34; with MOPS -4.6 +0.1 +0.2 -0.3 -0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -4.7 +0.1 +0.0 +0.0 -0.6
carbon source Valeric (C) -4.3 -0.0 +0.2 +0.1 -0.7
carbon source Tween 20 (C) -5.4 -0.2 +0.6 +0.1 +0.0
carbon source Octanoic (C) -3.9 -0.2 +0.0 -0.2 -0.5
pH Growth at pH6 and (C) D-Glucose -4.7 -0.2 -0.1 +0.1 +0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -4.7 +0.1 +0.2 -0.1 +0.0
pH Growth at pH6 and (C) Trisodium citrate -4.5 +0.1 -0.0 +0.1 +0.1
nitrogen source Urea (N); with MOPS -4.6 +0.0 +0.4 -0.1 +0.0
temperature Growth at 25C -3.5 -0.1 -0.4 -0.0 -0.2
pH D-Glucose (C) pH 6 -3.3 -0.2 -0.1 -0.3 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -4.5 -0.2 +0.2 +0.1 +0.5
stress MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL -3.2 +0.1 -0.2 -0.2 -0.4
nitrogen source NAG (N); with MOPS -4.6 +0.1 -0.1 +0.2 +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days -4.0 -0.4 +0.4 +0.2 -0.0
phage P. putida Missley MOI 0.1 -4.0 -0.0 +0.3 +0.3 -0.3
micoeukaryotes C.elegans, mixed culture-2 -4.3 +0.3 -0.0 +0.1 +0.2
stress R2A with Polymyxin B sulfate 0.002 mg/ml -4.3 +0.1 +0.0 +0.3 +0.2
pH D-Glucose (C) pH 6.5 -3.1 +0.0 +0.0 -0.6 +0.0
no phage control Time6-nophage -3.6 +0.0 +0.1 +0.0 +0.1
stress MOPS minimal media_Glucose20mM with Lithium chloride 200 mM -3.3 +0.2 -0.0 -0.0 -0.3
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -2.5 -0.1 +0.1 +0.2 -1.0
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -3.3 -0.0 +0.1 +0.4 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -3.2 +0.2 +0.3 +0.1 -0.4
pH pH 8 -3.3 -0.2 +0.2 +0.0 +0.3
biofilm mediium pdms biofilm mediium PDMS; 7 hours -3.4 +0.2 +0.1 -0.1 +0.3
attachment on pdms attachment on PDMS; 2 hours -3.2 +0.2 +0.4 +0.2 -0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs -3.8 +0.2 -0.1 +0.9 +0.3
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