Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4649 and PP_4650 are separated by 9 nucleotidesPP_4650 and PP_4651 are separated by 3 nucleotidesPP_4651 and PP_4652 are separated by 343 nucleotidesPP_4652 and PP_4653 are separated by 229 nucleotides PP_4649: PP_4649 - conserved protein of unknown function, at 5,274,084 to 5,274,239 _4649 PP_4650: PP_4650 - Ubiquinol oxidase subunit II, cyanide insensitive, at 5,274,249 to 5,275,256 _4650 PP_4651: PP_4651 - Ubiquinol oxidase subunit I, cyanide insensitive, at 5,275,260 to 5,276,696 _4651 PP_4652: PP_4652 - putative Membrane protein, at 5,277,040 to 5,278,260 _4652 PP_4653: PP_4653 - adenine:H+ symporter, at 5,278,490 to 5,279,785 _4653
Group Condition PP_4649 PP_4650 PP_4651 PP_4652 PP_4653
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -1.9 -3.3 +0.1 -0.2
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -1.9 -2.3 +0.2 -0.1
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -1.4 -1.1 +0.6 +0.1
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -1.0 -1.0 +0.5 -0.1
nitrogen source DL-2-Aminobutyric (N) N.D. +0.1 +0.0 -0.3 -1.4
carbon source carbon source 3-O-methyl-glucose 50 mM N.D. -0.5 -0.1 -0.7 -0.2
nitrogen source D-2-Aminobutyric (N) N.D. -0.2 +0.1 +0.0 -1.3
nitrogen source DL-2-Aminobutyric (N) N.D. -0.1 +0.0 -0.2 -1.1
carbon source acetate (C) N.D. -0.4 -0.2 -0.5 -0.2
carbon source carbon source D-Glucose 55.5 mM N.D. -0.0 -0.3 -1.1 +0.1
pH pH 5 N.D. -0.5 -0.3 -0.7 +0.2
carbon source carbon source 2-Deoxy-D-glucose 10 mM N.D. -0.2 -0.4 -0.8 +0.0
reactor 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. N.D. -1.2 -0.3 +0.3 -0.1
pH pH 6 N.D. -0.3 -0.2 -0.8 +0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs N.D. +0.4 -0.5 -0.6 -0.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.1 +0.2 -0.8 -0.5
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -0.8 -0.8 +0.4 +0.2
nitrogen source Urea (N); with MOPS N.D. +0.2 +0.2 -0.8 -0.5
nitrogen source D-2-Aminobutyric (N) N.D. +0.3 -0.2 +0.5 -1.3
pH pH 9 N.D. -0.2 -0.4 -0.3 +0.6
pH Growth at pH6 and (C) Trisodium citrate N.D. +0.5 +0.3 -0.8 -0.1
nitrogen source 2,6-diaminopimelic (N) N.D. -0.6 +0.1 +0.4 +0.4
phage P. putida South MOI 0.1 N.D. -0.4 -0.0 -0.2 +1.1
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.2 -0.3 +0.7 +0.3
phage P. putida PUT10 MOI 10 N.D. -0.2 +0.2 -0.3 +1.0
phage P. put South MOI 1 N.D. -0.3 -0.0 +0.2 +1.0
phage P. put PUT 10 MOI 0.1 N.D. -0.2 +0.2 +0.3 +0.7
phage P. putida PUT10 MOI 0.1 N.D. -0.1 -0.0 +0.2 +1.0
phage P. putida PUT10 MOI 10 N.D. -0.1 -0.0 +0.3 +1.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs N.D. +0.4 +0.1 +0.7 +0.2
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