Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4631 and PP_4632 are separated by 33 nucleotidesPP_4632 and PP_4633 are separated by 9 nucleotidesPP_4633 and PP_5703 overlap by 8 nucleotidesPP_5703 and PP_4634 are separated by 44 nucleotides PP_4631: PP_4631 - conserved protein of unknown function, at 5,254,877 to 5,255,437 _4631 PP_4632: PP_4632 - dihydrofolate reductase / dihydromonapterin reductase, at 5,255,471 to 5,256,208 _4632 PP_4633: PP_4633 - conserved protein of unknown function, DUF1244 family, at 5,256,218 to 5,256,514 _4633 PP_5703: PP_5703 - putative type 3 effector HopPmaJ, at 5,256,507 to 5,256,848 _5703 PP_4634: PP_4634 - putative hydrolase, alpha/beta fold family, at 5,256,893 to 5,257,801 _4634
Group Condition PP_4631 PP_4632 PP_4633 PP_5703 PP_4634
nitrogen source L-Isoleucine (N) -2.2 -0.3 -0.2 +0.1 -0.0
nitrogen source 2,6-diaminopimelic (N) +0.1 -1.4 -0.3 -0.9 -0.1
pH pH 9 -0.9 -0.7 -0.8 +0.0 +0.0
nitrogen source L-Isoleucine (N) -2.0 -0.2 -0.1 -0.2 +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.1 -0.2 -0.2 -0.1 -0.5
nitrogen source D-2-Aminobutyric (N) -0.4 -1.7 +0.6 +0.1 -0.6
pH pH 8 -0.8 -0.6 -0.5 -0.0 -0.2
nitrogen source DL-2-Aminobutyric (N) -0.5 -1.8 +0.5 -0.1 -0.0
carbon source 3-methyl-1-butanol 0.1 vol% (C) +0.2 -2.0 +0.4 +0.1 -0.5
nitrogen source L-Leucine (N) -1.1 -0.4 +0.2 -0.3 -0.1
nitrogen source 2,6-diaminopimelic (N) -0.0 -0.4 +0.3 -1.0 -0.6
nitrogen source DL-2-Aminobutyric (N) -0.0 -1.8 +0.3 -0.0 -0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 -0.3 -0.9 -0.5 -0.0
nitrogen source nitrogen source nitrite 0.5mM +0.4 -0.3 -1.0 +0.1 -0.4
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 -0.6 -0.5 -0.3 +0.6
carbon source carbon source D-Glucose 55.5 mM -0.5 -1.1 -0.1 +0.5 +0.2
pH Growth at pH6 and (C) Trisodium citrate -0.4 +0.4 -0.7 -0.3 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.7 -0.8 -0.1 +0.1 -0.5
phage P. putida PUT 7 MOI 1 +0.9 -0.6 -0.6 +0.1 -0.2
pH Growth at pH7 and (C) D-Glucose +0.3 +0.5 -0.0 -0.4 -0.8
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs +1.0 -0.7 +0.0 -0.1 -0.5
stress R2A with Polymyxin B sulfate 0.002 mg/ml +1.1 -0.2 +0.3 -0.7 -0.2
nitrogen source D-2-Aminobutyric (N) -0.3 -1.0 +0.9 +0.2 +0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.6 -0.4 +0.8 -0.2 -0.2
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.3 -1.1 -0.1 +0.3 +0.3
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.7 -0.2 +0.9 -0.1 -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.8 +0.2 -0.2 +0.7 -0.3
phage PUT 8 MOI 1 +0.7 -0.3 +0.4 +0.4 +0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 -0.1 +0.8 +0.8 -0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +1.9 +0.0 -0.0 +0.7 -0.1
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