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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_4631 and PP_4632 are separated by 33 nucleotides
PP_4632 and PP_4633 are separated by 9 nucleotides
PP_4633 and PP_5703 overlap by 8 nucleotides
PP_5703 and PP_4634 are separated by 44 nucleotides
PP_4631: PP_4631 - conserved protein of unknown function, at 5,254,877 to 5,255,437
_4631
PP_4632: PP_4632 - dihydrofolate reductase / dihydromonapterin reductase, at 5,255,471 to 5,256,208
_4632
PP_4633: PP_4633 - conserved protein of unknown function, DUF1244 family, at 5,256,218 to 5,256,514
_4633
PP_5703: PP_5703 - putative type 3 effector HopPmaJ, at 5,256,507 to 5,256,848
_5703
PP_4634: PP_4634 - putative hydrolase, alpha/beta fold family, at 5,256,893 to 5,257,801
_4634
Group
Condition
PP
_4631
PP
_4632
PP
_4633
PP
_5703
PP
_4634
nitrogen source
L-Isoleucine (N)
-2.2
-0.3
-0.2
+0.1
-0.0
nitrogen source
2,6-diaminopimelic (N)
+0.1
-1.4
-0.3
-0.9
-0.1
pH
pH 9
-0.9
-0.7
-0.8
+0.0
+0.0
nitrogen source
L-Isoleucine (N)
-2.0
-0.2
-0.1
-0.2
+0.1
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-1.1
-0.2
-0.2
-0.1
-0.5
nitrogen source
D-2-Aminobutyric (N)
-0.4
-1.7
+0.6
+0.1
-0.6
pH
pH 8
-0.8
-0.6
-0.5
-0.0
-0.2
nitrogen source
DL-2-Aminobutyric (N)
-0.5
-1.8
+0.5
-0.1
-0.0
carbon source
3-methyl-1-butanol 0.1 vol% (C)
+0.2
-2.0
+0.4
+0.1
-0.5
nitrogen source
L-Leucine (N)
-1.1
-0.4
+0.2
-0.3
-0.1
nitrogen source
2,6-diaminopimelic (N)
-0.0
-0.4
+0.3
-1.0
-0.6
nitrogen source
DL-2-Aminobutyric (N)
-0.0
-1.8
+0.3
-0.0
-0.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.3
-0.3
-0.9
-0.5
-0.0
nitrogen source
nitrogen source nitrite 0.5mM
+0.4
-0.3
-1.0
+0.1
-0.4
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.1
-0.6
-0.5
-0.3
+0.6
carbon source
carbon source D-Glucose 55.5 mM
-0.5
-1.1
-0.1
+0.5
+0.2
pH
Growth at pH6 and (C) Trisodium citrate
-0.4
+0.4
-0.7
-0.3
+0.3
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.7
-0.8
-0.1
+0.1
-0.5
phage
P. putida PUT 7 MOI 1
+0.9
-0.6
-0.6
+0.1
-0.2
pH
Growth at pH7 and (C) D-Glucose
+0.3
+0.5
-0.0
-0.4
-0.8
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
+1.0
-0.7
+0.0
-0.1
-0.5
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
+1.1
-0.2
+0.3
-0.7
-0.2
nitrogen source
D-2-Aminobutyric (N)
-0.3
-1.0
+0.9
+0.2
+0.6
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
+0.6
-0.4
+0.8
-0.2
-0.2
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+1.3
-1.1
-0.1
+0.3
+0.3
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.7
-0.2
+0.9
-0.1
-0.1
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.8
+0.2
-0.2
+0.7
-0.3
phage
PUT 8 MOI 1
+0.7
-0.3
+0.4
+0.4
+0.3
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.3
-0.1
+0.8
+0.8
-0.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
+1.9
+0.0
-0.0
+0.7
-0.1
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