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Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_5700 and PP_4614 are separated by 23 nucleotides
PP_4614 and PP_4615 are separated by 3 nucleotides
PP_4615 and PP_5701 are separated by 87 nucleotides
PP_5701 and PP_4616 are separated by 280 nucleotides
PP_5700: PP_5700 - conserved protein of unknown function, at 5,237,170 to 5,237,517
_5700
PP_4614: PP_4614 - conserved protein of unknown function, at 5,237,541 to 5,237,927
_4614
PP_4615: PP_4615 - conserved membrane protein of unknown function, at 5,237,931 to 5,238,401
_4615
PP_5701: PP_5701 - conserved protein of unknown function, at 5,238,489 to 5,238,692
_5701
PP_4616: PP_4616 - conserved protein of unknown function, at 5,238,973 to 5,239,236
_4616
Group
Condition
PP
_5700
PP
_4614
PP
_4615
PP
_5701
PP
_4616
temperature
Growth at 30C; with MOPS
N.D.
N.D.
+0.1
-6.3
-0.4
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
N.D.
N.D.
+0.2
-4.6
-0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
N.D.
N.D.
+0.2
-4.5
-0.2
carbon source
L-Proline (C)
N.D.
N.D.
-0.3
-3.7
-0.2
pH
Growth at pH8 and (C) D-Glucose
N.D.
N.D.
-0.0
-3.3
-0.4
pH
Growth at pH6 and (C) Trisodium citrate
N.D.
N.D.
+0.3
-3.6
-0.3
pH
Growth at pH8 and (C) Trisodium citrate
N.D.
N.D.
-0.1
-3.5
-0.1
phage
PUT 8 MOI 1
N.D.
N.D.
-0.0
-3.9
+0.4
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
N.D.
N.D.
-0.0
-3.0
-0.2
nitrogen source
no extra (N); with MOPS
N.D.
N.D.
-0.1
-2.6
-0.5
temperature shift
Temperature shift 30_to_21; with MOPS
N.D.
N.D.
-0.1
-3.2
+0.1
nitrogen source
nitrate (N)
N.D.
N.D.
+0.2
-3.5
+0.3
micoeukaryotes
Acanthamoeba sp., muxed culture-2
N.D.
N.D.
-0.3
-2.2
-0.1
nitrogen source
2,6-diaminopimelic (N)
N.D.
N.D.
+0.2
-1.9
-0.7
carbon source
Succinic Acid (C)
N.D.
N.D.
+0.1
-2.8
+0.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
N.D.
N.D.
-0.2
-1.9
-0.2
carbon source
3-Hydroxydecanoic (C)
N.D.
N.D.
+0.1
-1.9
-0.3
carbon source
D-Glucose; D-Xylose (C)
N.D.
N.D.
+0.2
-1.9
-0.2
temperature shift
Temperature shift 30_to_25
N.D.
N.D.
+0.2
-2.3
+0.3
carbon source
carbon source D-Glucose 55.5 mM
N.D.
N.D.
+0.1
-2.2
+0.3
nitrogen source
Propandiamine (N)
N.D.
N.D.
-0.4
-2.0
+0.8
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
N.D.
N.D.
+0.0
+0.6
-1.7
nitrogen source
nitrogen source nitrite 1mM
N.D.
N.D.
+0.1
-1.6
+0.6
carbon source
Quinic Acid (C)
N.D.
N.D.
+0.1
-1.5
+0.7
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
N.D.
-0.1
-1.3
+1.1
carbon source
ferulate (C); with MOPS
N.D.
N.D.
-0.8
+1.2
-0.7
nitrogen source
2,6-diaminopimelic (N)
N.D.
N.D.
+0.4
-2.2
+1.5
nitrogen source
D-2-Aminobutyric (N)
N.D.
N.D.
-1.7
+0.8
+0.9
nitrogen source
D-2-Aminobutyric (N)
N.D.
N.D.
-1.4
+1.4
+1.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
N.D.
-0.3
+2.8
+0.4
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