Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_5700 and PP_4614 are separated by 23 nucleotidesPP_4614 and PP_4615 are separated by 3 nucleotidesPP_4615 and PP_5701 are separated by 87 nucleotidesPP_5701 and PP_4616 are separated by 280 nucleotides PP_5700: PP_5700 - conserved protein of unknown function, at 5,237,170 to 5,237,517 _5700 PP_4614: PP_4614 - conserved protein of unknown function, at 5,237,541 to 5,237,927 _4614 PP_4615: PP_4615 - conserved membrane protein of unknown function, at 5,237,931 to 5,238,401 _4615 PP_5701: PP_5701 - conserved protein of unknown function, at 5,238,489 to 5,238,692 _5701 PP_4616: PP_4616 - conserved protein of unknown function, at 5,238,973 to 5,239,236 _4616
Group Condition PP_5700 PP_4614 PP_4615 PP_5701 PP_4616
temperature Growth at 30C; with MOPS N.D. N.D. +0.1 -6.3 -0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs N.D. N.D. +0.2 -4.6 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. +0.2 -4.5 -0.2
carbon source L-Proline (C) N.D. N.D. -0.3 -3.7 -0.2
pH Growth at pH8 and (C) D-Glucose N.D. N.D. -0.0 -3.3 -0.4
pH Growth at pH6 and (C) Trisodium citrate N.D. N.D. +0.3 -3.6 -0.3
pH Growth at pH8 and (C) Trisodium citrate N.D. N.D. -0.1 -3.5 -0.1
phage PUT 8 MOI 1 N.D. N.D. -0.0 -3.9 +0.4
stress R2A with Polymyxin B sulfate 0.002 mg/ml N.D. N.D. -0.0 -3.0 -0.2
nitrogen source no extra (N); with MOPS N.D. N.D. -0.1 -2.6 -0.5
temperature shift Temperature shift 30_to_21; with MOPS N.D. N.D. -0.1 -3.2 +0.1
nitrogen source nitrate (N) N.D. N.D. +0.2 -3.5 +0.3
micoeukaryotes Acanthamoeba sp., muxed culture-2 N.D. N.D. -0.3 -2.2 -0.1
nitrogen source 2,6-diaminopimelic (N) N.D. N.D. +0.2 -1.9 -0.7
carbon source Succinic Acid (C) N.D. N.D. +0.1 -2.8 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs N.D. N.D. -0.2 -1.9 -0.2
carbon source 3-Hydroxydecanoic (C) N.D. N.D. +0.1 -1.9 -0.3
carbon source D-Glucose; D-Xylose (C) N.D. N.D. +0.2 -1.9 -0.2
temperature shift Temperature shift 30_to_25 N.D. N.D. +0.2 -2.3 +0.3
carbon source carbon source D-Glucose 55.5 mM N.D. N.D. +0.1 -2.2 +0.3
nitrogen source Propandiamine (N) N.D. N.D. -0.4 -2.0 +0.8
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs N.D. N.D. +0.0 +0.6 -1.7
nitrogen source nitrogen source nitrite 1mM N.D. N.D. +0.1 -1.6 +0.6
carbon source Quinic Acid (C) N.D. N.D. +0.1 -1.5 +0.7
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. -0.1 -1.3 +1.1
carbon source ferulate (C); with MOPS N.D. N.D. -0.8 +1.2 -0.7
nitrogen source 2,6-diaminopimelic (N) N.D. N.D. +0.4 -2.2 +1.5
nitrogen source D-2-Aminobutyric (N) N.D. N.D. -1.7 +0.8 +0.9
nitrogen source D-2-Aminobutyric (N) N.D. N.D. -1.4 +1.4 +1.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. -0.3 +2.8 +0.4
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