Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4613 and PP_5700 are separated by 178 nucleotidesPP_5700 and PP_4614 are separated by 23 nucleotidesPP_4614 and PP_4615 are separated by 3 nucleotidesPP_4615 and PP_5701 are separated by 87 nucleotides PP_4613: PP_4613 - outer membrane ferric citrate porin, at 5,234,667 to 5,236,991 _4613 PP_5700: PP_5700 - conserved protein of unknown function, at 5,237,170 to 5,237,517 _5700 PP_4614: PP_4614 - conserved protein of unknown function, at 5,237,541 to 5,237,927 _4614 PP_4615: PP_4615 - conserved membrane protein of unknown function, at 5,237,931 to 5,238,401 _4615 PP_5701: PP_5701 - conserved protein of unknown function, at 5,238,489 to 5,238,692 _5701
Group Condition PP_4613 PP_5700 PP_4614 PP_4615 PP_5701
temperature Growth at 30C; with MOPS +0.1 N.D. N.D. +0.1 -6.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -0.1 N.D. N.D. +0.2 -4.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 N.D. N.D. +0.2 -4.5
pH Growth at pH8 and (C) Trisodium citrate -0.6 N.D. N.D. -0.1 -3.5
carbon source L-Proline (C) -0.1 N.D. N.D. -0.3 -3.7
phage PUT 8 MOI 1 +0.1 N.D. N.D. -0.0 -3.9
pH Growth at pH8 and (C) D-Glucose +0.0 N.D. N.D. -0.0 -3.3
temperature shift Temperature shift 30_to_21; with MOPS -0.0 N.D. N.D. -0.1 -3.2
pH Growth at pH6 and (C) Trisodium citrate +0.0 N.D. N.D. +0.3 -3.6
nitrogen source nitrate (N) +0.1 N.D. N.D. +0.2 -3.5
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.1 N.D. N.D. -0.0 -3.0
nitrogen source no extra (N); with MOPS -0.1 N.D. N.D. -0.1 -2.6
carbon source Succinic Acid (C) +0.0 N.D. N.D. +0.1 -2.8
micoeukaryotes Acanthamoeba sp., muxed culture-2 -0.1 N.D. N.D. -0.3 -2.2
nitrogen source Propandiamine (N) -0.2 N.D. N.D. -0.4 -2.0
mixed carbon source mixed (C)s Trisodium citrate 10 mM and L-Meta-tyrosine 10 mM -0.6 N.D. N.D. -0.1 -1.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -0.4 N.D. N.D. +0.1 -1.9
carbon source 3-methyl-1-butanol (C) -1.4 N.D. N.D. -0.2 -0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.1 N.D. N.D. -0.2 -1.9
temperature shift Temperature shift 30_to_25 -0.0 N.D. N.D. +0.2 -2.3
nitrogen source 2,6-diaminopimelic (N) -0.2 N.D. N.D. +0.2 -1.9
temperature shift Temperature shift 30_to_10; with MOPS -0.1 N.D. N.D. +0.1 -1.9
carbon source 3-Hydroxydecanoic (C) -0.1 N.D. N.D. +0.1 -1.9
carbon source D-Glucose; D-Xylose (C) -0.1 N.D. N.D. +0.2 -1.9
carbon source carbon source D-Glucose 55.5 mM +0.2 N.D. N.D. +0.1 -2.2
carbon source Soil Extract with D-Glucose 10 mM; 30C; then outgrowth in LB -1.8 N.D. N.D. -0.2 +0.2
nitrogen source 2,6-diaminopimelic (N) +0.3 N.D. N.D. +0.4 -2.2
nitrogen source D-2-Aminobutyric (N) -0.0 N.D. N.D. -1.7 +0.8
nitrogen source D-2-Aminobutyric (N) -0.1 N.D. N.D. -1.4 +1.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 N.D. N.D. -0.3 +2.8
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