Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_5699 and PP_4611 are separated by 139 nucleotidesPP_4611 and PP_4612 overlap by 4 nucleotidesPP_4612 and PP_4613 are separated by 94 nucleotidesPP_4613 and PP_5700 are separated by 178 nucleotides PP_5699: PP_5699 - putative iron uptake protein, at 5,232,692 to 5,232,997 _5699 PP_4611: PP_4611 - RNA polymerase sigma-70 factor, ECF subfamily, at 5,233,137 to 5,233,649 _4611 PP_4612: PP_4612 - putative FecR protein, at 5,233,646 to 5,234,572 _4612 PP_4613: PP_4613 - outer membrane ferric citrate porin, at 5,234,667 to 5,236,991 _4613 PP_5700: PP_5700 - conserved protein of unknown function, at 5,237,170 to 5,237,517 _5700
Group Condition PP_5699 PP_4611 PP_4612 PP_4613 PP_5700
mixed carbon source mixed (C)s Trisodium citrate 10 mM and 3-Chloro-L-tyrosine 10 mM -0.3 -1.6 +0.1 -1.7 N.D.
carbon source 3-methyl-1-butanol (C) +0.6 -1.3 -1.0 -1.4 N.D.
carbon source Soil Extract with D-Glucose 10 mM; 30C; then outgrowth in LB +0.2 -1.2 -0.1 -1.5 N.D.
carbon source Soil Extract with D-Glucose 10 mM; 30C; then outgrowth in LB +0.5 -1.3 +0.0 -1.8 N.D.
carbon source 3-methyl-1-butanol 0.1 vol% (C) +0.7 -1.6 -0.5 -1.1 N.D.
carbon source M9 minimal media_noCarbon with Trisodium citrate 10 mM; 30C; then outgrowth in LB +0.5 -1.3 -0.5 -1.3 N.D.
stress 2-Ethylhexanol 0.4 vol% -0.6 -0.9 -0.1 -0.9 N.D.
carbon source M9 minimal media_noCarbon with Trisodium citrate 10 mM; 30C; then outgrowth in LB +0.3 -1.1 -0.4 -1.1 N.D.
mixed carbon source mixed (C)s Trisodium citrate 10 mM and L-tyrosine 5 mM -0.1 -1.1 -0.2 -0.8 N.D.
carbon source 3-methyl-1-butanol (C) +0.4 -0.6 -0.8 -1.0 N.D.
carbon source M9 minimal media_noCarbon with Trisodium citrate 10 mM; 30C +0.5 -1.0 -0.3 -1.1 N.D.
carbon source M9 minimal media_noCarbon with Trisodium citrate 10 mM; 30C +0.4 -1.0 -0.3 -1.1 N.D.
carbon source Soil Extract with Trisodium citrate 10 mM; 30C +0.3 -0.9 -0.0 -1.3 N.D.
carbon source Soil Extract with Trisodium citrate 10 mM; 30C +0.4 -1.0 +0.1 -1.2 N.D.
pH Growth at pH8 and (C) Trisodium citrate -0.1 -0.5 +0.0 -1.2 N.D.
mixed carbon source mixed (C)s Trisodium citrate 10 mM and 3-Chloro-L-tyrosine 10 mM -0.1 -0.8 +0.2 -1.0 N.D.
mixed carbon source mixed (C)s Trisodium citrate 10 mM and 4-azido-L-phenylalanine 10 mM -0.2 -0.6 +0.1 -0.8 N.D.
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -2.0 +0.1 +0.3 +0.0 N.D.
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -1.7 -0.1 +0.2 +0.0 N.D.
mixed carbon source mixed (C)s Trisodium citrate 10 mM and 4-azido-L-phenylalanine 10 mM +0.2 -0.8 +0.1 -1.0 N.D.
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -1.9 +0.1 +0.3 +0.0 N.D.
pH Growth at pH8 and (C) Trisodium citrate +0.3 -0.8 -0.3 -0.6 N.D.
stress R2A with Fusaric 480 ug/mL -1.2 +0.2 -0.2 -0.0 N.D.
stress Growth with Chloride 500 mM; with MOPS -0.9 +0.2 -0.4 +0.1 N.D.
stress R2A with Fusaric 480 ug/mL -1.3 +0.3 -0.0 +0.1 N.D.
pH pH 6 -0.8 +0.8 +0.2 +0.2 N.D.
carbon source carbon source 4-vinylphenol 0.1 mM -0.4 +1.0 -0.1 +0.4 N.D.
carbon source carbon source 2-Deoxy-D-glucose 100 mM -0.4 +1.1 +0.1 +0.3 N.D.
pH pH 7 -0.5 +1.0 +0.3 +0.4 N.D.
carbon source carbon source 2-Deoxy-D-glucose 50 mM -0.4 +1.3 +0.1 +0.4 N.D.
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