Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4594 and PP_4595 are separated by 59 nucleotidesPP_4595 and PP_4596 are separated by 109 nucleotidesPP_4596 and PP_4597 are separated by 222 nucleotidesPP_4597 and PP_4598 are separated by 20 nucleotides PP_4594: PP_4594 - putative Cystathionine gamma-synthase, at 5,212,530 to 5,213,711 _4594 PP_4595: PP_4595 - putative LRP-like transcriptional regulator, at 5,213,771 to 5,214,265 _4595 PP_4596: PP_4596 - putative oxidoreductase, at 5,214,375 to 5,216,705 _4596 PP_4597: PP_4597 - Cyclic pyranopterin monophosphate synthase, at 5,216,928 to 5,217,932 _4597 PP_4598: PP_4598 - conserved membrane protein of unknown function, at 5,217,953 to 5,218,852 _4598
Group Condition PP_4594 PP_4595 PP_4596 PP_4597 PP_4598
nitrogen source nitrate (N) +0.0 +0.4 +0.1 -3.6 -0.0
nitrogen source nitrogen source nitrate 5mM -0.1 -0.0 -0.2 -2.7 +0.2
carbon source Vanillin (C) +0.3 -0.0 -0.0 -2.9 +0.1
carbon source Vanillin (C) +0.1 +0.2 -0.1 -2.9 +0.2
carbon source carbon source 3-O-methyl-glucose 50 mM -0.2 -0.4 +0.0 -1.4 -0.3
carbon source Vanillin (C) +0.2 +0.1 +0.1 -2.4 -0.0
nitrogen source nitrate (N) +0.1 -0.0 -0.0 -2.0 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.2 -0.4 -0.0 -0.9 -0.3
nitrogen source nitrate (N) +0.0 +0.1 +0.0 -2.0 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.4 -0.6 +0.2 -0.7 -0.0
nitrogen source nitrogen source nitrate 20mM +0.1 +0.1 -0.0 -1.8 +0.0
nitrogen source Ammonium chloride (N); with MOPS -0.1 -0.2 +0.2 -1.3 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.4 +0.1 +0.1 -0.8 -0.4
carbon source Vanillin (C) +0.1 +0.3 -0.1 -1.9 +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days +0.2 -0.7 +0.1 -1.0 +0.2
nitrogen source NAG (N); with MOPS +0.2 +0.3 +0.1 -1.6 -0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.0 +0.3 -0.1 -1.2 -0.2
nitrogen source NAG (N) +0.2 +0.3 +0.1 -1.3 -0.2
nitrogen source Propiolactam (N) -0.2 -0.3 +0.1 -1.0 +0.4
nitrogen source 2,6-diaminopimelic (N) +0.1 +0.7 -0.1 -0.9 -0.4
nitrogen source nitrogen source nitrite 0.5mM -0.2 -0.2 +0.2 +0.8 -0.7
pH Growth at pH7 and (C) Trisodium citrate +0.4 -0.5 -0.3 -0.2 +0.6
phage P. putida Sci small MOI 0.1 +0.6 -0.4 +0.3 -0.5 +0.2
nitrogen source 2,6-diaminopimelic (N) +0.0 +0.1 +0.2 +0.7 -0.8
micoeukaryotes Tetrahymena sp., Mixed culture-2 +0.3 +0.1 +0.0 -0.7 +0.7
pH Valerolactone (C) pH 6.5 +0.4 +0.6 -0.3 -0.3 +0.3
nitrogen source L-Methionine (N) -0.2 +1.3 -0.1 -0.3 -0.1
nitrogen source L-Methionine (N) -0.3 +1.4 -0.2 -0.1 -0.1
no stress control Growth with no stress +0.5 +0.6 -0.0 +0.4 -0.4
carbon source ferulate (C); with MOPS +0.9 +0.3 +0.3 +0.6 +0.2
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