Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4592 and PP_4593 are separated by 250 nucleotidesPP_4593 and PP_4594 are separated by 93 nucleotidesPP_4594 and PP_4595 are separated by 59 nucleotidesPP_4595 and PP_4596 are separated by 109 nucleotides PP_4592: PP_4592 - putative metal dependent hydrolase, at 5,210,312 to 5,211,949 _4592 PP_4593: PP_4593 - conserved protein of unknown function, at 5,212,200 to 5,212,436 _4593 PP_4594: PP_4594 - putative Cystathionine gamma-synthase, at 5,212,530 to 5,213,711 _4594 PP_4595: PP_4595 - putative LRP-like transcriptional regulator, at 5,213,771 to 5,214,265 _4595 PP_4596: PP_4596 - putative oxidoreductase, at 5,214,375 to 5,216,705 _4596
Group Condition PP_4592 PP_4593 PP_4594 PP_4595 PP_4596
phage P. putida Missley MOI 0.1 -0.0 -4.6 -0.1 -0.3 -0.1
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -1.7 -2.2 -0.6 -0.2 -0.3
micoeukaryotes C.elegans, mixed culture-2 -0.0 -4.3 +0.1 -0.1 -0.1
stress R2A with Polymyxin B sulfate 0.001 mg/ml -1.0 -2.4 -0.4 -0.2 -0.1
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -1.7 -1.6 -0.4 -0.3 -0.1
carbon source Tween 20 (C) -0.1 -4.1 -0.0 +0.2 -0.0
nitrogen source NAG (N); with MOPS +0.0 -4.5 +0.2 +0.3 +0.1
temperature shift Temperature shift 30_to_21; with MOPS +0.1 -4.4 -0.0 +0.7 -0.2
carbon source 3-methyl-1-butanol 0.25 vol% (C) +0.1 -2.7 -0.3 -0.5 -0.1
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -2.0 -0.7 -0.4 -0.2 -0.1
pH Growth at pH6 and (C) D-Glucose +0.0 -3.6 +0.3 -0.0 +0.1
pH Growth at pH6 and (C) Trisodium citrate +0.0 -3.3 -0.0 +0.3 +0.1
temperature shift Temperature shift 10_to_25; with MOPS +0.2 -3.0 -0.0 +0.0 -0.1
carbon source Nonanoic (C) -0.0 -3.2 +0.4 +0.2 -0.2
pH pH 5 -0.1 -2.7 +0.2 +0.0 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs -0.0 -2.3 -0.1 +0.0 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.3 -2.4 -0.6 +0.0 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.1 -2.0 -0.2 -0.2 +0.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 -2.0 +0.0 -0.0 -0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days -1.0 -0.7 +0.2 -0.7 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.3 -1.9 -0.4 +0.1 +0.1
pH Growth at pH7 and (C) Trisodium citrate +0.1 -1.5 +0.4 -0.5 -0.3
temperature shift Temperature shift 10_to_30; with MOPS +0.0 -2.3 +0.3 +0.2 +0.2
nitrogen source D-2-Aminobutyric (N) +0.3 -2.2 +0.5 +0.1 -0.1
temperature shift Temperature shift 10_to_21; with MOPS +0.1 -1.8 +0.3 +0.4 -0.1
stress R2A with Polymyxin B sulfate 0.001 mg/ml -0.7 +1.6 -0.1 -0.3 -0.1
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO. +0.3 +1.6 +0.2 -0.3 -0.1
pH Valerolactone (C) pH 6.5 +0.3 +1.5 +0.4 +0.6 -0.3
pH D-Glucose (C) pH 6.5 +0.0 +2.0 +0.1 +0.2 +0.2
stress MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL -0.0 +2.3 +0.2 +0.0 +0.1
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