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Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_4592 and PP_4593 are separated by 250 nucleotides
PP_4593 and PP_4594 are separated by 93 nucleotides
PP_4594 and PP_4595 are separated by 59 nucleotides
PP_4595 and PP_4596 are separated by 109 nucleotides
PP_4592: PP_4592 - putative metal dependent hydrolase, at 5,210,312 to 5,211,949
_4592
PP_4593: PP_4593 - conserved protein of unknown function, at 5,212,200 to 5,212,436
_4593
PP_4594: PP_4594 - putative Cystathionine gamma-synthase, at 5,212,530 to 5,213,711
_4594
PP_4595: PP_4595 - putative LRP-like transcriptional regulator, at 5,213,771 to 5,214,265
_4595
PP_4596: PP_4596 - putative oxidoreductase, at 5,214,375 to 5,216,705
_4596
Group
Condition
PP
_4592
PP
_4593
PP
_4594
PP
_4595
PP
_4596
phage
P. putida Missley MOI 0.1
-0.0
-4.6
-0.1
-0.3
-0.1
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
-1.7
-2.2
-0.6
-0.2
-0.3
micoeukaryotes
C.elegans, mixed culture-2
-0.0
-4.3
+0.1
-0.1
-0.1
stress
R2A with Polymyxin B sulfate 0.001 mg/ml
-1.0
-2.4
-0.4
-0.2
-0.1
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
-1.7
-1.6
-0.4
-0.3
-0.1
carbon source
Tween 20 (C)
-0.1
-4.1
-0.0
+0.2
-0.0
nitrogen source
NAG (N); with MOPS
+0.0
-4.5
+0.2
+0.3
+0.1
temperature shift
Temperature shift 30_to_21; with MOPS
+0.1
-4.4
-0.0
+0.7
-0.2
carbon source
3-methyl-1-butanol 0.25 vol% (C)
+0.1
-2.7
-0.3
-0.5
-0.1
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
-2.0
-0.7
-0.4
-0.2
-0.1
pH
Growth at pH6 and (C) D-Glucose
+0.0
-3.6
+0.3
-0.0
+0.1
pH
Growth at pH6 and (C) Trisodium citrate
+0.0
-3.3
-0.0
+0.3
+0.1
temperature shift
Temperature shift 10_to_25; with MOPS
+0.2
-3.0
-0.0
+0.0
-0.1
carbon source
Nonanoic (C)
-0.0
-3.2
+0.4
+0.2
-0.2
pH
pH 5
-0.1
-2.7
+0.2
+0.0
+0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs
-0.0
-2.3
-0.1
+0.0
-0.2
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
+0.3
-2.4
-0.6
+0.0
+0.2
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
+0.1
-2.0
-0.2
-0.2
+0.0
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.2
-2.0
+0.0
-0.0
-0.4
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days
-1.0
-0.7
+0.2
-0.7
+0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
+0.3
-1.9
-0.4
+0.1
+0.1
pH
Growth at pH7 and (C) Trisodium citrate
+0.1
-1.5
+0.4
-0.5
-0.3
temperature shift
Temperature shift 10_to_30; with MOPS
+0.0
-2.3
+0.3
+0.2
+0.2
nitrogen source
D-2-Aminobutyric (N)
+0.3
-2.2
+0.5
+0.1
-0.1
temperature shift
Temperature shift 10_to_21; with MOPS
+0.1
-1.8
+0.3
+0.4
-0.1
stress
R2A with Polymyxin B sulfate 0.001 mg/ml
-0.7
+1.6
-0.1
-0.3
-0.1
reactor
72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
+0.3
+1.6
+0.2
-0.3
-0.1
pH
Valerolactone (C) pH 6.5
+0.3
+1.5
+0.4
+0.6
-0.3
pH
D-Glucose (C) pH 6.5
+0.0
+2.0
+0.1
+0.2
+0.2
stress
MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL
-0.0
+2.3
+0.2
+0.0
+0.1
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