Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4559 and PP_4560 overlap by 1 nucleotidesPP_4560 and PP_4561 are separated by 153 nucleotidesPP_4561 and PP_4562 are separated by 235 nucleotidesPP_4562 and PP_4563 are separated by 5 nucleotides PP_4559: PP_4559 - peptide deformylase, at 5,180,450 to 5,180,986 _4559 PP_4560: PP_4560 - putative Ribonuclease BN, at 5,180,986 to 5,181,942 _4560 PP_4561: PP_4561 - stress response protein, at 5,182,096 to 5,182,275 _4561 PP_4562: PP_4562 - conserved protein of unknown function, at 5,182,511 to 5,182,891 _4562 PP_4563: PP_4563 - conserved protein of unknown function, at 5,182,897 to 5,183,733 _4563
Group Condition PP_4559 PP_4560 PP_4561 PP_4562 PP_4563
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.4 -1.8 -5.4 N.D. -0.5
stress R2A with Fusaric 480 ug/mL +0.1 -1.6 -5.3 N.D. -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.9 -0.9 -4.8 N.D. -0.0
stress Growth with Chloride 500 mM; with MOPS -0.0 -1.4 -4.8 N.D. -0.3
pH Growth at pH7 and (C) Trisodium citrate -0.7 -1.0 -4.5 N.D. -0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.6 -0.3 -4.6 N.D. -0.5
nitrogen source nitrogen source nitrite 1mM +0.4 -1.8 -4.2 N.D. -0.1
carbon source 3-methyl-1-butanol 0.25 vol% (C) -0.0 -3.2 -1.9 N.D. -0.4
nitrogen source Urea (N) -0.2 -0.4 -4.5 N.D. -0.1
pH Growth at pH6 and (C) D-Glucose -0.2 -0.2 -4.6 N.D. -0.2
temperature shift Temperature shift 30_to_25; with MOPS +0.1 -0.9 -4.4 N.D. +0.1
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -0.1 -3.3 -1.6 N.D. -0.1
carbon source D-Glucose (C) -0.2 -0.3 -4.7 N.D. +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.1 -1.6 -3.4 N.D. +0.0
temperature Growth at 30C +0.1 +0.2 -4.7 N.D. -0.0
r2a control with 0.2% methanol R2A control with 0.2% methanol -0.1 -2.7 -1.7 N.D. +0.0
r2a control R2A control -0.6 -3.3 -0.5 N.D. +0.1
temperature Growth at 25C -0.7 +0.0 -2.7 N.D. -0.7
nitrogen source Urea (N) +0.3 -0.1 -4.5 N.D. +0.2
nitrogen source Ammonium chloride (N) -0.6 -1.0 -2.5 N.D. +0.3
r2a control R2A control +0.4 -1.3 -2.9 N.D. -0.1
carbon source Myristic (C) -0.4 -0.7 -2.9 N.D. +0.2
pH Growth at pH6 and (C) D-Glucose -1.2 -0.9 -2.0 N.D. +0.3
temperature shift Temperature shift 10_to_30; with MOPS -0.5 +1.2 -3.7 N.D. -0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.7 +0.6 -3.6 N.D. -0.5
carbon source L-Phenylalanine 5 mM (C) +0.1 +0.6 -3.3 N.D. +0.2
pH pH 4 +0.9 -0.2 -3.0 N.D. +0.1
carbon source carbon source 4-vinylphenol 0.1 mM +0.5 +1.2 -2.5 N.D. -0.2
pH pH 8 +1.0 +0.7 -2.6 N.D. +0.2
reactor 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. -0.3 +1.8 -2.2 N.D. -0.1
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