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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_4559 and PP_4560 overlap by 1 nucleotides
PP_4560 and PP_4561 are separated by 153 nucleotides
PP_4561 and PP_4562 are separated by 235 nucleotides
PP_4562 and PP_4563 are separated by 5 nucleotides
PP_4559: PP_4559 - peptide deformylase, at 5,180,450 to 5,180,986
_4559
PP_4560: PP_4560 - putative Ribonuclease BN, at 5,180,986 to 5,181,942
_4560
PP_4561: PP_4561 - stress response protein, at 5,182,096 to 5,182,275
_4561
PP_4562: PP_4562 - conserved protein of unknown function, at 5,182,511 to 5,182,891
_4562
PP_4563: PP_4563 - conserved protein of unknown function, at 5,182,897 to 5,183,733
_4563
Group
Condition
PP
_4559
PP
_4560
PP
_4561
PP
_4562
PP
_4563
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-0.4
-1.8
-5.4
N.D.
-0.5
stress
R2A with Fusaric 480 ug/mL
+0.1
-1.6
-5.3
N.D.
-0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
-0.9
-0.9
-4.8
N.D.
-0.0
stress
Growth with Chloride 500 mM; with MOPS
-0.0
-1.4
-4.8
N.D.
-0.3
pH
Growth at pH7 and (C) Trisodium citrate
-0.7
-1.0
-4.5
N.D.
-0.0
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-0.6
-0.3
-4.6
N.D.
-0.5
nitrogen source
nitrogen source nitrite 1mM
+0.4
-1.8
-4.2
N.D.
-0.1
carbon source
3-methyl-1-butanol 0.25 vol% (C)
-0.0
-3.2
-1.9
N.D.
-0.4
nitrogen source
Urea (N)
-0.2
-0.4
-4.5
N.D.
-0.1
pH
Growth at pH6 and (C) D-Glucose
-0.2
-0.2
-4.6
N.D.
-0.2
temperature shift
Temperature shift 30_to_25; with MOPS
+0.1
-0.9
-4.4
N.D.
+0.1
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
-0.1
-3.3
-1.6
N.D.
-0.1
carbon source
D-Glucose (C)
-0.2
-0.3
-4.7
N.D.
+0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
-0.1
-1.6
-3.4
N.D.
+0.0
temperature
Growth at 30C
+0.1
+0.2
-4.7
N.D.
-0.0
r2a control with 0.2% methanol
R2A control with 0.2% methanol
-0.1
-2.7
-1.7
N.D.
+0.0
r2a control
R2A control
-0.6
-3.3
-0.5
N.D.
+0.1
temperature
Growth at 25C
-0.7
+0.0
-2.7
N.D.
-0.7
nitrogen source
Urea (N)
+0.3
-0.1
-4.5
N.D.
+0.2
nitrogen source
Ammonium chloride (N)
-0.6
-1.0
-2.5
N.D.
+0.3
r2a control
R2A control
+0.4
-1.3
-2.9
N.D.
-0.1
carbon source
Myristic (C)
-0.4
-0.7
-2.9
N.D.
+0.2
pH
Growth at pH6 and (C) D-Glucose
-1.2
-0.9
-2.0
N.D.
+0.3
temperature shift
Temperature shift 10_to_30; with MOPS
-0.5
+1.2
-3.7
N.D.
-0.3
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.7
+0.6
-3.6
N.D.
-0.5
carbon source
L-Phenylalanine 5 mM (C)
+0.1
+0.6
-3.3
N.D.
+0.2
pH
pH 4
+0.9
-0.2
-3.0
N.D.
+0.1
carbon source
carbon source 4-vinylphenol 0.1 mM
+0.5
+1.2
-2.5
N.D.
-0.2
pH
pH 8
+1.0
+0.7
-2.6
N.D.
+0.2
reactor
24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
-0.3
+1.8
-2.2
N.D.
-0.1
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