Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4552 and PP_4553 are separated by 116 nucleotidesPP_4553 and PP_4554 are separated by 17 nucleotidesPP_4554 and PP_4555 are separated by 21 nucleotidesPP_4555 and PP_4556 are separated by 44 nucleotides PP_4552: PP_4552 - putative conserved enzyme of unknown function, at 5,176,177 to 5,176,647 _4552 PP_4553: PP_4553 - RNA polymerase sigma-70 factor, ECF subfamily, at 5,176,764 to 5,177,984 _4553 PP_4554: PP_4554 - conserved protein of unknown function, at 5,178,002 to 5,178,487 _4554 PP_4555: PP_4555 - conserved protein of unknown function, at 5,178,509 to 5,178,853 _4555 PP_4556: PP_4556 - conserved protein of unknown function, at 5,178,898 to 5,179,323 _4556
Group Condition PP_4552 PP_4553 PP_4554 PP_4555 PP_4556
stress R2A with Polymyxin B sulfate 0.002 mg/ml N.D. -0.1 N.D. -2.6 -2.0
phage P. putida Sci Big MOI 0.1 N.D. -0.6 N.D. -3.8 -0.2
pH Growth at pH7 and (C) D-Glucose N.D. -0.6 N.D. -3.3 +0.1
pH Growth at pH8 and (C) D-Glucose N.D. -0.1 N.D. -1.8 -1.3
pH Growth at pH9 and (C) Trisodium citrate N.D. -0.4 N.D. -2.8 +0.0
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. N.D. -0.1 N.D. -2.3 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +0.2 N.D. -2.6 -0.5
nitrogen source Urea (N); with MOPS N.D. +0.1 N.D. -2.0 -0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.4 N.D. -1.6 -1.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.0 N.D. -2.6 +0.2
temperature Growth at 25C N.D. -0.4 N.D. -0.5 -1.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs N.D. -0.1 N.D. -2.4 +0.4
pH Growth at pH6 and (C) Trisodium citrate N.D. +0.2 N.D. -0.4 -1.8
carbon source 1,3-Butandiol (C) N.D. -0.4 N.D. -1.3 -0.3
carbon source Nonanoic (C) N.D. -0.3 N.D. +0.1 -1.7
pH Growth at pH6 and (C) Trisodium citrate N.D. -0.2 N.D. -1.7 +0.1
nitrogen source Urea (N) N.D. -0.3 N.D. +0.3 -1.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. +0.2 N.D. -1.6 +0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days N.D. -0.1 N.D. -1.4 +0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs N.D. +0.0 N.D. -1.3 +0.7
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.7 N.D. -0.5 -0.8
nitrogen source NAG (N); with MOPS N.D. -0.3 N.D. +1.0 -1.2
no stress control Growth with no stress N.D. +0.2 N.D. +0.7 -1.1
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.1 N.D. +1.1 -1.1
phage PUT 8 MOI 1 N.D. +0.1 N.D. +1.2 -1.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.1 N.D. -0.7 +1.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days N.D. +0.0 N.D. +1.5 -1.0
carbon source L-Leucine (C) N.D. -0.0 N.D. +1.5 -0.6
nitrogen source 2,6-diaminopimelic (N) N.D. -0.1 N.D. +0.6 +1.4
nitrogen source D-2-Aminobutyric (N) N.D. +0.4 N.D. +1.7 +0.7
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