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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_4552 and PP_4553 are separated by 116 nucleotides
PP_4553 and PP_4554 are separated by 17 nucleotides
PP_4554 and PP_4555 are separated by 21 nucleotides
PP_4555 and PP_4556 are separated by 44 nucleotides
PP_4552: PP_4552 - putative conserved enzyme of unknown function, at 5,176,177 to 5,176,647
_4552
PP_4553: PP_4553 - RNA polymerase sigma-70 factor, ECF subfamily, at 5,176,764 to 5,177,984
_4553
PP_4554: PP_4554 - conserved protein of unknown function, at 5,178,002 to 5,178,487
_4554
PP_4555: PP_4555 - conserved protein of unknown function, at 5,178,509 to 5,178,853
_4555
PP_4556: PP_4556 - conserved protein of unknown function, at 5,178,898 to 5,179,323
_4556
Group
Condition
PP
_4552
PP
_4553
PP
_4554
PP
_4555
PP
_4556
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
N.D.
-0.1
N.D.
-2.6
-2.0
phage
P. putida Sci Big MOI 0.1
N.D.
-0.6
N.D.
-3.8
-0.2
pH
Growth at pH7 and (C) D-Glucose
N.D.
-0.6
N.D.
-3.3
+0.1
pH
Growth at pH8 and (C) D-Glucose
N.D.
-0.1
N.D.
-1.8
-1.3
pH
Growth at pH9 and (C) Trisodium citrate
N.D.
-0.4
N.D.
-2.8
+0.0
reactor
24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
N.D.
-0.1
N.D.
-2.3
-0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
N.D.
+0.2
N.D.
-2.6
-0.5
nitrogen source
Urea (N); with MOPS
N.D.
+0.1
N.D.
-2.0
-0.7
agar plate interaction control
Parafilmed volatile agar plate with no fungus
N.D.
+0.4
N.D.
-1.6
-1.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
N.D.
-0.0
N.D.
-2.6
+0.2
temperature
Growth at 25C
N.D.
-0.4
N.D.
-0.5
-1.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
N.D.
-0.1
N.D.
-2.4
+0.4
pH
Growth at pH6 and (C) Trisodium citrate
N.D.
+0.2
N.D.
-0.4
-1.8
carbon source
1,3-Butandiol (C)
N.D.
-0.4
N.D.
-1.3
-0.3
carbon source
Nonanoic (C)
N.D.
-0.3
N.D.
+0.1
-1.7
pH
Growth at pH6 and (C) Trisodium citrate
N.D.
-0.2
N.D.
-1.7
+0.1
nitrogen source
Urea (N)
N.D.
-0.3
N.D.
+0.3
-1.6
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
N.D.
+0.2
N.D.
-1.6
+0.3
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
N.D.
-0.1
N.D.
-1.4
+0.5
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
N.D.
+0.0
N.D.
-1.3
+0.7
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
+0.7
N.D.
-0.5
-0.8
nitrogen source
NAG (N); with MOPS
N.D.
-0.3
N.D.
+1.0
-1.2
no stress control
Growth with no stress
N.D.
+0.2
N.D.
+0.7
-1.1
in planta
Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
N.D.
+0.1
N.D.
+1.1
-1.1
phage
PUT 8 MOI 1
N.D.
+0.1
N.D.
+1.2
-1.0
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
-0.1
N.D.
-0.7
+1.2
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
N.D.
+0.0
N.D.
+1.5
-1.0
carbon source
L-Leucine (C)
N.D.
-0.0
N.D.
+1.5
-0.6
nitrogen source
2,6-diaminopimelic (N)
N.D.
-0.1
N.D.
+0.6
+1.4
nitrogen source
D-2-Aminobutyric (N)
N.D.
+0.4
N.D.
+1.7
+0.7
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