Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4544 and PP_4545 are separated by 200 nucleotidesPP_4545 and PP_4546 are separated by 123 nucleotidesPP_4546 and PP_4547 are separated by 503 nucleotidesPP_4547 and PP_4548 are separated by 24 nucleotides PP_4544: PP_4544 - conserved exported protein of unknown function, at 5,161,233 to 5,162,477 _4544 PP_4545: PP_4545 - beta-ketodecanoyl-(Acyl-carrier-protein) synthase, at 5,162,678 to 5,163,850 _4545 PP_4546: PP_4546 - ATP-dependent helicase HrpA, at 5,163,974 to 5,167,879 _4546 PP_4547: PP_4547 - putative Glutamine synthetase, at 5,168,383 to 5,169,747 _4547 PP_4548: PP_4548 - putative Oxidoreductase, at 5,169,772 to 5,171,079 _4548
Group Condition PP_4544 PP_4545 PP_4546 PP_4547 PP_4548
nitrogen source Propandiamine (N) -0.3 +0.7 -1.2 -5.3 -5.7
nitrogen source Propandiamine (N) +0.1 +0.8 -1.1 -5.9 -5.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs -0.1 -0.5 -2.8 -0.2 -0.0
carbon source Decanoic (C) -0.0 -1.2 -0.4 -0.8 -1.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.0 -1.4 -2.5 +0.1 +0.2
carbon source Delta-Undecalactone (C) -0.1 -1.6 -1.6 -0.1 -0.2
carbon source Octanoic (C) +0.0 -0.7 -1.8 -0.3 -0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.0 -1.0 -2.2 -0.1 -0.0
carbon source 3-methyl-1-butanol 0.1 vol% (C) -0.1 -0.7 -1.7 -0.4 -0.4
carbon source Decanoic (C) -0.0 -1.1 -0.8 -0.4 -0.9
temperature Growth at 30C; with MOPS +0.0 -0.2 -2.4 -0.3 -0.3
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.2 -1.0 -2.1 -0.0 -0.2
pH Growth at pH6 and (C) D-Glucose -0.3 -0.4 -2.0 -0.1 -0.3
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.1 -1.3 -1.6 -0.1 -0.1
supernatant control:fungal media R2A with Vogels_fungal_media 0.2X -0.1 +0.0 -2.8 -0.0 -0.1
carbon source Nonanoic (C) -0.2 -0.1 -1.1 -0.6 -1.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.1 +0.2 -2.2 -0.5 -0.3
carbon source 5-Hydroxydodecanoate (C) +0.1 -1.3 -1.3 -0.0 -0.3
nitrogen source NAG (N); with MOPS -0.1 +0.1 -2.1 -0.4 -0.3
carbon source Delta-Undecalactone (C) +0.0 -1.2 -1.5 +0.3 -0.3
pH Growth at pH7 and (C) D-Glucose -0.1 +0.2 -1.9 -0.5 -0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -0.0 -0.6 -2.3 +0.2 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs +0.2 +0.6 -3.0 -0.2 -0.1
carbon source 2,3-Butanediol (C) +0.3 -0.7 -1.0 -0.3 -0.7
nitrogen source Urea (N) -0.1 +0.2 -2.6 +0.1 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.1 +0.5 -2.4 -0.0 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.1 +0.1 -1.9 +0.3 -0.6
r2a control with 0.2% methanol R2A control with 0.2% methanol +0.2 +0.5 -1.9 -0.1 -0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs +0.4 +0.1 -2.1 +0.2 -0.3
nitrogen source D-2-Aminobutyric (N) -0.2 +1.4 -1.0 -0.5 -0.1
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