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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_4544 and PP_4545 are separated by 200 nucleotides
PP_4545 and PP_4546 are separated by 123 nucleotides
PP_4546 and PP_4547 are separated by 503 nucleotides
PP_4547 and PP_4548 are separated by 24 nucleotides
PP_4544: PP_4544 - conserved exported protein of unknown function, at 5,161,233 to 5,162,477
_4544
PP_4545: PP_4545 - beta-ketodecanoyl-(Acyl-carrier-protein) synthase, at 5,162,678 to 5,163,850
_4545
PP_4546: PP_4546 - ATP-dependent helicase HrpA, at 5,163,974 to 5,167,879
_4546
PP_4547: PP_4547 - putative Glutamine synthetase, at 5,168,383 to 5,169,747
_4547
PP_4548: PP_4548 - putative Oxidoreductase, at 5,169,772 to 5,171,079
_4548
Group
Condition
PP
_4544
PP
_4545
PP
_4546
PP
_4547
PP
_4548
nitrogen source
Propandiamine (N)
-0.3
+0.7
-1.2
-5.3
-5.7
nitrogen source
Propandiamine (N)
+0.1
+0.8
-1.1
-5.9
-5.5
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs
-0.1
-0.5
-2.8
-0.2
-0.0
carbon source
Decanoic (C)
-0.0
-1.2
-0.4
-0.8
-1.2
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
+0.0
-1.4
-2.5
+0.1
+0.2
carbon source
Delta-Undecalactone (C)
-0.1
-1.6
-1.6
-0.1
-0.2
carbon source
Octanoic (C)
+0.0
-0.7
-1.8
-0.3
-0.6
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
-0.0
-1.0
-2.2
-0.1
-0.0
carbon source
3-methyl-1-butanol 0.1 vol% (C)
-0.1
-0.7
-1.7
-0.4
-0.4
carbon source
Decanoic (C)
-0.0
-1.1
-0.8
-0.4
-0.9
temperature
Growth at 30C; with MOPS
+0.0
-0.2
-2.4
-0.3
-0.3
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.2
-1.0
-2.1
-0.0
-0.2
pH
Growth at pH6 and (C) D-Glucose
-0.3
-0.4
-2.0
-0.1
-0.3
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.1
-1.3
-1.6
-0.1
-0.1
supernatant control:fungal media
R2A with Vogels_fungal_media 0.2X
-0.1
+0.0
-2.8
-0.0
-0.1
carbon source
Nonanoic (C)
-0.2
-0.1
-1.1
-0.6
-1.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
-0.1
+0.2
-2.2
-0.5
-0.3
carbon source
5-Hydroxydodecanoate (C)
+0.1
-1.3
-1.3
-0.0
-0.3
nitrogen source
NAG (N); with MOPS
-0.1
+0.1
-2.1
-0.4
-0.3
carbon source
Delta-Undecalactone (C)
+0.0
-1.2
-1.5
+0.3
-0.3
pH
Growth at pH7 and (C) D-Glucose
-0.1
+0.2
-1.9
-0.5
-0.4
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
-0.0
-0.6
-2.3
+0.2
+0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
+0.2
+0.6
-3.0
-0.2
-0.1
carbon source
2,3-Butanediol (C)
+0.3
-0.7
-1.0
-0.3
-0.7
nitrogen source
Urea (N)
-0.1
+0.2
-2.6
+0.1
+0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
-0.1
+0.5
-2.4
-0.0
-0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
+0.1
+0.1
-1.9
+0.3
-0.6
r2a control with 0.2% methanol
R2A control with 0.2% methanol
+0.2
+0.5
-1.9
-0.1
-0.4
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
+0.4
+0.1
-2.1
+0.2
-0.3
nitrogen source
D-2-Aminobutyric (N)
-0.2
+1.4
-1.0
-0.5
-0.1
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