Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4526 and PP_4527 are separated by 18 nucleotidesPP_4527 and PP_4528 are separated by 105 nucleotidesPP_4528 and PP_4529 are separated by 40 nucleotidesPP_4529 and PP_4530 overlap by 23 nucleotides PP_4526: PP_4526 - putative MFS superfamily transporter precursor, at 5,141,666 to 5,142,838 _4526 PP_4527: PP_4527 - putative permease of the drug/metabolite transporter (DMT) superfamily, at 5,142,857 to 5,143,783 _4527 PP_4528: PP_4528 - conserved protein of unknown function, at 5,143,889 to 5,144,320 _4528 PP_4529: PP_4529 - conserved protein of unknown function, at 5,144,361 to 5,144,615 _4529 PP_4530: PP_4530 - conserved protein of unknown function, at 5,144,593 to 5,144,871 _4530
Group Condition PP_4526 PP_4527 PP_4528 PP_4529 PP_4530
pH Growth at pH6 and (C) D-Glucose -0.1 -0.3 -4.4 -0.5 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.2 +0.1 -4.8 +0.5 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.2 -0.2 -3.5 -0.1 +0.2
nitrogen source D-2-Aminobutyric (N) +0.2 -0.4 -2.4 -0.2 -0.2
pH Growth at pH6 and (C) D-Glucose -0.1 -0.2 -2.6 +0.2 -0.2
carbon source 3-methyl-1-butanol 0.25 vol% (C) -0.3 +0.1 -1.6 -1.0 +0.1
carbon source L-tyrosine 5 mM (C) -0.1 +0.1 -1.7 -0.5 -0.1
temperature shift Temperature shift 10_to_30; with MOPS -0.2 +0.1 -1.7 -0.0 -0.4
carbon source 1,3-Hexanediol (C) -0.2 +0.1 -2.3 +0.3 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs +0.3 +0.1 -2.0 -0.3 -0.1
nitrogen source NAG (N) -0.0 +0.2 -0.9 -1.2 +0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 -0.6 -0.2 -1.3 +0.1
temperature shift Temperature shift 10_to_25 +0.1 +0.3 -0.7 -1.1 -0.2
carbon source Valerolactone (C) -0.2 -0.2 +0.6 -1.7 +0.0
phage P. put PUT 10 MOI 10 +0.1 -0.0 +0.5 -1.6 -0.3
pH Growth at pH7 and (C) D-Glucose -0.1 -0.1 +0.4 -1.8 +0.3
temperature shift Temperature shift 30_to_4 +0.1 +0.4 -0.4 -1.4 +0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 -0.3 -1.3 -0.2 +0.7
stress R2A with Polymyxin B sulfate 0.001 mg/ml +0.4 -0.3 -1.5 +0.0 +0.3
temperature Growth at 30C +0.2 -0.0 +0.7 -1.6 +0.0
pH D-Glucose (C) pH 7.5 +0.2 +0.1 -1.6 +0.5 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.1 +0.8 -1.1 -0.3 +0.4
nitrogen source Urea (N) +0.1 -0.1 +0.7 -1.3 +0.4
nitrogen source nitrogen source nitrate 5mM -0.2 +0.4 -1.5 +1.0 +0.3
nitrogen source nitrate (N) -0.7 +0.1 +1.0 -0.7 +0.3
pH pH 9 +0.3 +0.1 +1.0 -1.1 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.3 +0.2 +0.8 -1.5 +0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.4 -0.3 +2.5 -0.6 -0.2
stress 2-Ethylhexanol 0.4 vol% -0.2 -0.2 +0.8 +1.5 -0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +0.3 +0.0 +1.5 +0.1 +0.5
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