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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_4457 and PP_4458 are separated by 0 nucleotides
PP_4458 and PP_4459 are separated by 490 nucleotides
PP_4459 and PP_4460 are separated by 197 nucleotides
PP_4460 and PP_4461 are separated by 158 nucleotides
PP_4457: PP_4457 - opine oxidase subunit B, at 5,058,125 to 5,059,252
_4457
PP_4458: PP_4458 - putative Opine ABC transporter, periplasmic binding protein, at 5,059,253 to 5,060,833
_4458
PP_4459: PP_4459 - transposase, at 5,061,324 to 5,062,421
_4459
PP_4460: PP_4460 - Transcriptional regulator, LysR family, at 5,062,619 to 5,063,545
_4460
PP_4461: PP_4461 - Major facilitator family transporter, at 5,063,704 to 5,064,996
_4461
Group
Condition
PP
_4457
PP
_4458
PP
_4459
PP
_4460
PP
_4461
nitrogen source
NAG (N); with MOPS
+0.2
-0.3
+0.2
-1.8
-0.2
pH
pH 7
-0.3
-0.2
-0.5
-0.2
-0.7
carbon source
carbon source 3-O-methyl-glucose 50 mM
-0.1
-0.2
-0.2
-0.5
-0.7
carbon source
carbon source 4-vinylphenol 0.1 mM
-0.3
-0.3
-0.1
-0.4
-0.4
reactor
48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
-0.1
-0.1
-0.3
-0.4
-0.6
carbon source
carbon source 2-Deoxy-D-glucose 50 mM
-0.1
-0.2
-0.6
-0.3
-0.3
carbon source
L-Alanine (C)
-0.3
-0.1
-0.3
-0.4
-0.4
pH
pH 6
-0.1
-0.2
+0.1
-0.6
-0.6
carbon source
D-Glucose (C)
-0.4
-0.3
+0.0
-0.5
-0.1
nitrogen source
no extra (N); with MOPS
-0.1
+0.0
-0.3
-0.3
-0.5
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.2
+0.2
-0.3
-0.5
+0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
+0.1
+0.2
-0.2
-0.9
+0.1
carbon source
ferulate (C); with MOPS
+0.1
-0.1
+0.2
-1.0
+0.2
nitrogen source
Propiolactam (N)
-0.2
-0.2
-0.3
+0.2
+0.4
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
+0.2
+0.1
+0.3
-0.6
-0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
-0.1
-0.1
-0.5
+0.3
+0.3
carbon source
Trisodium citrate (C)
+0.0
+0.1
+0.1
-0.7
+0.4
carbon source
Crotonic (C)
-0.1
-0.1
-0.3
-0.2
+0.7
carbon source
5-Aminovaleric 10 mM (C)
-0.2
-0.2
-0.1
+0.4
+0.4
carbon source
Valerolactone (C)
-0.1
-0.2
+0.2
+0.8
-0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
+0.3
-0.2
+0.2
+0.3
+0.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
+0.2
+0.3
+0.4
+0.1
+0.3
carbon source
3-methyl-1-butanol 0.1 vol% (C)
+0.3
+0.3
+0.3
+0.1
+0.4
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs
+0.5
+0.2
+0.1
+0.0
+0.6
phage
P. putida Sci small MOI 0.1
+0.1
+0.4
+0.2
+0.4
+0.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
+0.5
+0.1
+0.3
+0.2
+0.4
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.1
+0.2
+0.3
+0.5
+0.6
phage
P. putida Sci small MOI 0.1
+0.1
+0.5
+0.6
+0.3
+0.4
phage
P. putida PUT 7 MOI 1
+0.7
+0.7
+0.6
+0.3
+0.8
phage
P. putida Missley MOI 0.1
+0.7
+0.7
+1.0
+0.9
+0.9
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