Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4457 and PP_4458 are separated by 0 nucleotidesPP_4458 and PP_4459 are separated by 490 nucleotidesPP_4459 and PP_4460 are separated by 197 nucleotidesPP_4460 and PP_4461 are separated by 158 nucleotides PP_4457: PP_4457 - opine oxidase subunit B, at 5,058,125 to 5,059,252 _4457 PP_4458: PP_4458 - putative Opine ABC transporter, periplasmic binding protein, at 5,059,253 to 5,060,833 _4458 PP_4459: PP_4459 - transposase, at 5,061,324 to 5,062,421 _4459 PP_4460: PP_4460 - Transcriptional regulator, LysR family, at 5,062,619 to 5,063,545 _4460 PP_4461: PP_4461 - Major facilitator family transporter, at 5,063,704 to 5,064,996 _4461
Group Condition PP_4457 PP_4458 PP_4459 PP_4460 PP_4461
nitrogen source NAG (N); with MOPS +0.2 -0.3 +0.2 -1.8 -0.2
pH pH 7 -0.3 -0.2 -0.5 -0.2 -0.7
carbon source carbon source 3-O-methyl-glucose 50 mM -0.1 -0.2 -0.2 -0.5 -0.7
carbon source carbon source 4-vinylphenol 0.1 mM -0.3 -0.3 -0.1 -0.4 -0.4
reactor 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -0.1 -0.1 -0.3 -0.4 -0.6
carbon source carbon source 2-Deoxy-D-glucose 50 mM -0.1 -0.2 -0.6 -0.3 -0.3
carbon source L-Alanine (C) -0.3 -0.1 -0.3 -0.4 -0.4
pH pH 6 -0.1 -0.2 +0.1 -0.6 -0.6
carbon source D-Glucose (C) -0.4 -0.3 +0.0 -0.5 -0.1
nitrogen source no extra (N); with MOPS -0.1 +0.0 -0.3 -0.3 -0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 +0.2 -0.3 -0.5 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.1 +0.2 -0.2 -0.9 +0.1
carbon source ferulate (C); with MOPS +0.1 -0.1 +0.2 -1.0 +0.2
nitrogen source Propiolactam (N) -0.2 -0.2 -0.3 +0.2 +0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs +0.2 +0.1 +0.3 -0.6 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.1 -0.1 -0.5 +0.3 +0.3
carbon source Trisodium citrate (C) +0.0 +0.1 +0.1 -0.7 +0.4
carbon source Crotonic (C) -0.1 -0.1 -0.3 -0.2 +0.7
carbon source 5-Aminovaleric 10 mM (C) -0.2 -0.2 -0.1 +0.4 +0.4
carbon source Valerolactone (C) -0.1 -0.2 +0.2 +0.8 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.3 -0.2 +0.2 +0.3 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.2 +0.3 +0.4 +0.1 +0.3
carbon source 3-methyl-1-butanol 0.1 vol% (C) +0.3 +0.3 +0.3 +0.1 +0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs +0.5 +0.2 +0.1 +0.0 +0.6
phage P. putida Sci small MOI 0.1 +0.1 +0.4 +0.2 +0.4 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs +0.5 +0.1 +0.3 +0.2 +0.4
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.1 +0.2 +0.3 +0.5 +0.6
phage P. putida Sci small MOI 0.1 +0.1 +0.5 +0.6 +0.3 +0.4
phage P. putida PUT 7 MOI 1 +0.7 +0.7 +0.6 +0.3 +0.8
phage P. putida Missley MOI 0.1 +0.7 +0.7 +1.0 +0.9 +0.9
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