Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4420 and PP_5679 are separated by 172 nucleotidesPP_5679 and PP_4421 are separated by 233 nucleotidesPP_4421 and PP_4422 are separated by 21 nucleotidesPP_4422 and PP_4423 are separated by 259 nucleotides PP_4420: PP_4420 - transposase, at 5,014,543 to 5,015,352 _4420 PP_5679: PP_5679 - protein of unknown function, at 5,015,525 to 5,015,737 _5679 PP_4421: PP_4421 - putative aminotransferase, at 5,015,971 to 5,017,362 _4421 PP_4422: PP_4422 - succinate-semialdehyde dehydrogenase (NADP+), at 5,017,384 to 5,018,856 _4422 PP_4423: PP_4423 - conserved protein of unknown function, at 5,019,116 to 5,020,111 _4423
Group Condition PP_4420 PP_5679 PP_4421 PP_4422 PP_4423
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.3 -0.4 -0.1 -0.3 -0.4
carbon source carbon source 3-O-methyl-glucose 50 mM -0.1 -0.1 -0.5 -0.3 -0.5
carbon source carbon source 2-Deoxy-D-glucose 10 mM -0.2 -0.2 -0.4 -0.3 -0.3
carbon source carbon source Bisabolene 48.9 mM -0.3 -0.2 -0.3 -0.3 -0.3
pH pH 6 +0.0 -0.4 -0.2 -0.3 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.2 -0.4 -0.0 -0.4 -0.5
nitrogen source 2,6-diaminopimelic (N) -0.0 -0.0 -0.1 +0.4 -1.0
temperature shift Temperature shift 30_to_34; with MOPS -0.2 -0.3 -0.1 -0.4 +0.3
nitrogen source nitrogen source nitrite 1mM +0.3 -0.4 -0.2 -0.1 -0.4
carbon source L-Leucine (C) +0.3 -0.3 +0.1 -0.1 -0.7
pH Growth at pH6 and (C) Trisodium citrate -0.3 -0.1 +0.0 -0.3 +0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.0 -0.4 +0.2 -0.3 +0.5
nitrogen source nitrate (N) +0.2 +0.4 +0.2 -0.2 -0.4
carbon source Valerolactone (C) -0.2 +0.5 +0.1 +0.2 -0.4
micoeukaryotes C.elegans, mixed culture-2 -0.1 -0.1 -0.2 +0.9 +0.0
carbon source 5-Aminovaleric 10 mM (C) +0.1 -0.2 -0.2 +0.8 +0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.3 +0.2 +0.3 -0.4 +0.7
carbon source Valerolactam 10 mM (C) +0.1 -0.2 -0.1 +1.3 +0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.1 +0.4 +0.3 +0.0 +0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.7 +0.3 +0.4 +0.2 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs +0.2 +0.5 +0.1 +0.5 +0.3
nitrogen source Caprolactam (N) +0.0 -0.2 -0.0 +1.8 +0.0
phage P. putida Sci small MOI 0.1 +0.0 +0.4 +0.2 +0.1 +0.9
nitrogen source 2,6-diaminopimelic (N) +0.3 +0.8 +0.0 +0.2 +0.4
nitrogen source Caprolactam (N) -0.0 +0.1 -0.0 +1.8 +0.2
phage P. putida Sci small MOI 0.1 +0.5 +0.6 +0.3 +0.4 +0.4
phage P. putida PUT 7 MOI 1 +0.6 +0.4 +0.7 +0.5 +0.8
nitrogen source Caprolactam (N) -0.0 -0.1 -0.0 +3.3 -0.0
nitrogen source Caprolactam (N) -0.0 -0.0 +0.1 +3.5 -0.2
phage P. putida Missley MOI 0.1 +0.9 +0.7 +0.6 +0.7 +0.5
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