Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4305 and PP_4306 are separated by 13 nucleotidesPP_4306 and PP_4307 are separated by 1 nucleotidesPP_4307 and PP_4308 are separated by 133 nucleotidesPP_4308 and PP_4309 are separated by 497 nucleotides PP_4305: PP_4305 - sulfate ABC transporter, at 4,896,575 to 4,897,573 _4305 PP_4306: PP_4306 - conserved protein of unknown function, at 4,897,587 to 4,898,543 _4306 PP_4307: PP_4307 - putative permease of the drug/metabolite transporter (DMT) superfamily, at 4,898,545 to 4,899,468 _4307 PP_4308: PP_4308 - Transcriptional regulator, AsnC family, at 4,899,602 to 4,900,039 _4308 PP_4309: PP_4309 - Transporter, NCS1 nucleoside transporter family, at 4,900,537 to 4,902,069 _4309
Group Condition PP_4305 PP_4306 PP_4307 PP_4308 PP_4309
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 -0.6 -0.3 -1.9 -0.0
phage P. putida Sci small MOI 0.1 -0.3 -1.4 -0.2 -0.0 -0.4
no stress control Growth with no stress +0.0 -0.6 +0.0 -2.0 +0.3
pH Growth at pH7 and (C) Trisodium citrate +0.2 -0.3 -0.3 -1.4 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.0 -0.2 -0.2 -1.3 +0.0
carbon source 3-methyl-1-butanol 0.25 vol% (C) -0.2 -0.8 -0.3 -0.2 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs +0.1 -0.2 +0.2 -1.4 +0.1
phage P. putida Sci Big MOI 0.1 -0.0 +0.3 -0.7 -0.3 -0.4
r2a control with 0.2% methanol R2A control with 0.2% methanol +0.2 -0.2 -0.2 -0.9 +0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.2 -0.4 +0.0 -1.0 +0.0
pH Growth at pH7 and (C) D-Glucose +0.2 -0.3 +0.1 -1.0 -0.1
nitrogen source Propandiamine (N) +0.2 -1.0 -0.2 +0.2 -0.2
temperature shift Temperature shift 30_to_25; with MOPS -0.1 +0.3 +0.2 -1.4 -0.0
nitrogen source Urea (N) +0.1 -0.6 +0.1 -0.7 +0.2
carbon source Myristic (C) +0.2 -0.7 +0.2 -0.8 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs +0.2 -0.3 -0.1 -0.9 +0.2
nitrogen source Ammonium chloride (N) +0.0 +0.4 +0.0 -1.2 +0.1
temperature shift Temperature shift 30_to_34; with MOPS +0.2 +0.2 +0.1 -0.9 -0.2
micoeukaryotes Acanthamoeba sp., muxed culture-1 +0.0 -0.9 -0.0 +0.4 +0.2
carbon source carbon source 3-O-methyl-glucose 50 mM -0.2 +0.4 -0.0 +0.5 -0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.3 -0.6 +0.4 -0.3 +0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.3 -0.4 +0.1 +1.1 -0.2
pH Growth at pH9 and (C) Trisodium citrate +0.0 -0.4 -0.3 +0.9 +0.2
nitrogen source Urea (N); with MOPS +0.4 +0.6 -0.4 +0.2 -0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.1 +0.6 +0.1 +0.5 -0.5
nitrogen source D-2-Aminobutyric (N) +0.3 -0.5 +0.1 +1.0 +0.1
nitrogen source NAG (N); with MOPS +0.3 +0.3 +0.2 +0.7 -0.1
phage P. putida South MOI 0.1 +0.5 +0.4 -0.1 +0.5 +0.2
nitrogen source D-2-Aminobutyric (N) +0.5 +0.0 -0.0 +1.1 -0.1
carbon source ferulate (C); with MOPS +0.0 +0.2 +0.3 +1.3 +0.1
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