Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4284 and PP_4285 are separated by 436 nucleotidesPP_4285 and PP_4286 are separated by 399 nucleotidesPP_4286 and PP_4287 overlap by 1 nucleotidesPP_4287 and PP_4288 are separated by 49 nucleotides PP_4284: PP_4284 - putative Transporter, at 4,874,813 to 4,876,162 _4284 PP_4285: PP_4285 - 5-hydroxyisourate hydrolase, at 4,876,599 to 4,876,952 _4285 PP_4286: PP_4286 - allantoinase, at 4,877,352 to 4,878,278 _4286 PP_4287: PP_4287 - 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase, at 4,878,278 to 4,878,793 _4287 PP_4288: PP_4288 - ureidoglycolate lyase, at 4,878,843 to 4,879,346 _4288
Group Condition PP_4284 PP_4285 PP_4286 PP_4287 PP_4288
nitrogen source Adenine (N) -0.0 -0.9 -1.4 -2.9 -0.4
nitrogen source Adenine (N) +0.1 -0.9 -1.4 -2.6 -0.4
nitrogen source D-2-Aminobutyric (N) -0.4 -1.7 -0.3 -1.3 -0.5
carbon source ferulate (C); with MOPS -0.1 -1.8 -1.1 -0.5 -0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.3 -0.7 -2.3 -0.5 +0.2
carbon source 3-methyl-1-butanol 0.1 vol% (C) -0.0 -1.5 -1.4 -0.4 +0.3
nitrogen source nitrogen source nitrite 1mM +0.2 -0.4 -1.2 -0.4 -0.9
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -0.1 -0.6 -0.9 -0.3 -0.5
temperature shift Temperature shift 30_to_21; with MOPS +0.1 -1.9 -0.2 -0.1 -0.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.1 +0.2 -2.0 +0.5 -1.3
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 -0.7 -1.3 -0.4 -0.4
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.3 -1.0 -0.7 +0.0 -0.4
micoeukaryotes Tetrahymena sp., Mixed culture-1 -0.1 -0.0 -1.7 -0.6 +0.0
temperature Growth at 30C; with MOPS -0.1 -0.9 +0.1 -0.5 -0.7
pH Growth at pH6 and (C) D-Glucose -0.6 -0.4 -0.6 +0.3 -0.8
nitrogen source nitrate (N) -0.1 -0.3 +0.3 -0.0 -1.8
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs +0.2 -0.2 -0.9 -0.4 -0.7
temperature shift Temperature shift 30_to_25; with MOPS +0.1 -1.8 +0.2 -0.3 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs +0.5 -2.0 -1.0 +0.4 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.3 -1.0 -0.7 +0.1 -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.1 -1.0 -0.5 -0.7 +0.5
mixed carbon source mixed (C)s Trisodium citrate 10 mM and L-tyrosine 5 mM -0.1 -2.1 +0.1 +0.3 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.3 -0.6 -1.1 -0.1 +0.5
temperature shift Temperature shift 30_to_25; with MOPS -0.3 +0.5 +0.0 -0.1 -1.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.3 -0.1 -0.3 +0.7 -1.8
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.1 -0.5 -0.9 -0.4 +0.4
micoeukaryotes Tetrahymena sp., Mixed culture-2 -0.2 +0.9 -1.3 +0.1 -0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs +0.3 -0.4 -1.3 +0.3 +0.6
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 +1.5 -1.7 -0.3 +0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 -0.5 -1.6 -0.0 +2.4
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