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Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_4284 and PP_4285 are separated by 436 nucleotides
PP_4285 and PP_4286 are separated by 399 nucleotides
PP_4286 and PP_4287 overlap by 1 nucleotides
PP_4287 and PP_4288 are separated by 49 nucleotides
PP_4284: PP_4284 - putative Transporter, at 4,874,813 to 4,876,162
_4284
PP_4285: PP_4285 - 5-hydroxyisourate hydrolase, at 4,876,599 to 4,876,952
_4285
PP_4286: PP_4286 - allantoinase, at 4,877,352 to 4,878,278
_4286
PP_4287: PP_4287 - 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase, at 4,878,278 to 4,878,793
_4287
PP_4288: PP_4288 - ureidoglycolate lyase, at 4,878,843 to 4,879,346
_4288
Group
Condition
PP
_4284
PP
_4285
PP
_4286
PP
_4287
PP
_4288
nitrogen source
Adenine (N)
-0.0
-0.9
-1.4
-2.9
-0.4
nitrogen source
Adenine (N)
+0.1
-0.9
-1.4
-2.6
-0.4
nitrogen source
D-2-Aminobutyric (N)
-0.4
-1.7
-0.3
-1.3
-0.5
carbon source
ferulate (C); with MOPS
-0.1
-1.8
-1.1
-0.5
-0.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.3
-0.7
-2.3
-0.5
+0.2
carbon source
3-methyl-1-butanol 0.1 vol% (C)
-0.0
-1.5
-1.4
-0.4
+0.3
nitrogen source
nitrogen source nitrite 1mM
+0.2
-0.4
-1.2
-0.4
-0.9
reactor
24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
-0.1
-0.6
-0.9
-0.3
-0.5
temperature shift
Temperature shift 30_to_21; with MOPS
+0.1
-1.9
-0.2
-0.1
-0.4
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.1
+0.2
-2.0
+0.5
-1.3
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.3
-0.7
-1.3
-0.4
-0.4
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.3
-1.0
-0.7
+0.0
-0.4
micoeukaryotes
Tetrahymena sp., Mixed culture-1
-0.1
-0.0
-1.7
-0.6
+0.0
temperature
Growth at 30C; with MOPS
-0.1
-0.9
+0.1
-0.5
-0.7
pH
Growth at pH6 and (C) D-Glucose
-0.6
-0.4
-0.6
+0.3
-0.8
nitrogen source
nitrate (N)
-0.1
-0.3
+0.3
-0.0
-1.8
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs
+0.2
-0.2
-0.9
-0.4
-0.7
temperature shift
Temperature shift 30_to_25; with MOPS
+0.1
-1.8
+0.2
-0.3
-0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
+0.5
-2.0
-1.0
+0.4
+0.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
+0.3
-1.0
-0.7
+0.1
-0.5
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.1
-1.0
-0.5
-0.7
+0.5
mixed carbon source
mixed (C)s Trisodium citrate 10 mM and L-tyrosine 5 mM
-0.1
-2.1
+0.1
+0.3
+0.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
-0.3
-0.6
-1.1
-0.1
+0.5
temperature shift
Temperature shift 30_to_25; with MOPS
-0.3
+0.5
+0.0
-0.1
-1.5
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
+0.3
-0.1
-0.3
+0.7
-1.8
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.1
-0.5
-0.9
-0.4
+0.4
micoeukaryotes
Tetrahymena sp., Mixed culture-2
-0.2
+0.9
-1.3
+0.1
-0.6
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
+0.3
-0.4
-1.3
+0.3
+0.6
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.2
+1.5
-1.7
-0.3
+0.3
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.1
-0.5
-1.6
-0.0
+2.4
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