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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_4260 and PP_4261 are separated by 136 nucleotides
PP_4261 and PP_4262 overlap by 22 nucleotides
PP_4262 and PP_4263 overlap by 8 nucleotides
PP_4263 and PP_4264 are separated by 105 nucleotides
PP_4260: PP_4260 - conserved protein of unknown function, at 4,844,584 to 4,845,114
_4260
PP_4261: PP_4261 - cation-transporting P-type ATPase, at 4,845,251 to 4,847,725
_4261
PP_4262: PP_4262 - Cytochrome oxidase maturation protein, cbb3-type, at 4,847,704 to 4,847,919
_4262
PP_4263: PP_4263 - putative membrane-bounded cytochrome biogenesis cycZ-like domain, copper tolerance protein, at 4,847,912 to 4,848,595
_4263
PP_4264: PP_4264 - oxygen-independent coproporphyrinogen III dehydrogenase, at 4,848,701 to 4,850,083
_4264
Group
Condition
PP
_4260
PP
_4261
PP
_4262
PP
_4263
PP
_4264
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
-0.2
-3.8
-1.6
-3.4
-1.1
stress
Geraniol 1 vol%
-1.5
-2.4
-2.8
-2.2
-1.2
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
-0.6
-1.6
-4.3
-2.4
-0.8
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
-0.6
-0.0
-2.9
-5.6
-0.5
micoeukaryotes
Tetrahymena sp., Mixed culture-1
-0.5
-2.1
-3.4
-2.3
-1.2
micoeukaryotes
C.elegans, mixed culture-1
-3.7
-2.0
-2.2
-0.6
-0.2
carbon source
ferulate (C); with MOPS
-1.2
-1.3
-3.5
-1.7
-0.3
nitrogen source
NAG (N); with MOPS
-0.1
-1.9
+0.1
-5.6
-0.4
micoeukaryotes
Tetrahymena sp., Mixed culture-2
-0.5
-3.8
+0.9
-2.9
-1.4
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
-0.5
-2.0
-1.6
-2.7
-0.7
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs
-0.6
-1.2
-2.0
-2.7
-0.9
lb
LB
-0.5
-1.7
-1.7
-2.2
-0.9
in planta
Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-1.4
-1.0
-2.3
-1.9
-0.6
in planta
Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-1.5
-1.4
-1.3
-1.7
-1.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
-0.6
-1.1
-3.6
-1.2
-0.5
carbon source
ferulate (C); with MOPS
-2.3
-1.1
-1.1
-2.0
-0.0
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
-0.9
-2.4
-2.0
-0.5
-0.5
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
-0.1
-1.4
-1.6
-2.4
-0.7
pH
Growth at pH7 and (C) D-Glucose
-0.6
+0.0
-4.2
-1.2
-0.1
lb
LB
-0.2
-1.8
-1.9
-1.3
-1.0
reactor
24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
-0.8
-1.1
-2.4
-1.0
-0.8
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.2
-1.7
-1.3
-1.8
-1.4
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
-0.4
-1.4
-2.7
-1.1
-0.2
carbon source
M9 minimal media_noCarbon with Trisodium citrate 10 mM; 30C; then outgrowth in LB
-0.4
-1.1
-2.3
-1.3
-0.7
pH
Growth at pH6 and (C) D-Glucose
+0.0
-0.4
-4.0
-1.3
-0.1
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-2.4
-0.5
-2.0
-0.8
+0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-4.2
-0.1
-0.5
+1.2
-0.4
stress
R2A with Fusaric 480 ug/mL
+0.1
+0.5
-2.6
+0.8
-2.0
supernatant control:fungal media
R2A with Vogels_fungal_media 0.8X
+1.2
+1.5
+1.2
+1.2
+0.9
reactor
72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay
+1.5
+2.2
+3.1
+1.2
+2.2
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