Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4260 and PP_4261 are separated by 136 nucleotidesPP_4261 and PP_4262 overlap by 22 nucleotidesPP_4262 and PP_4263 overlap by 8 nucleotidesPP_4263 and PP_4264 are separated by 105 nucleotides PP_4260: PP_4260 - conserved protein of unknown function, at 4,844,584 to 4,845,114 _4260 PP_4261: PP_4261 - cation-transporting P-type ATPase, at 4,845,251 to 4,847,725 _4261 PP_4262: PP_4262 - Cytochrome oxidase maturation protein, cbb3-type, at 4,847,704 to 4,847,919 _4262 PP_4263: PP_4263 - putative membrane-bounded cytochrome biogenesis cycZ-like domain, copper tolerance protein, at 4,847,912 to 4,848,595 _4263 PP_4264: PP_4264 - oxygen-independent coproporphyrinogen III dehydrogenase, at 4,848,701 to 4,850,083 _4264
Group Condition PP_4260 PP_4261 PP_4262 PP_4263 PP_4264
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.2 -3.8 -1.6 -3.4 -1.1
stress Geraniol 1 vol% -1.5 -2.4 -2.8 -2.2 -1.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -0.6 -1.6 -4.3 -2.4 -0.8
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.6 -0.0 -2.9 -5.6 -0.5
micoeukaryotes Tetrahymena sp., Mixed culture-1 -0.5 -2.1 -3.4 -2.3 -1.2
micoeukaryotes C.elegans, mixed culture-1 -3.7 -2.0 -2.2 -0.6 -0.2
carbon source ferulate (C); with MOPS -1.2 -1.3 -3.5 -1.7 -0.3
nitrogen source NAG (N); with MOPS -0.1 -1.9 +0.1 -5.6 -0.4
micoeukaryotes Tetrahymena sp., Mixed culture-2 -0.5 -3.8 +0.9 -2.9 -1.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -0.5 -2.0 -1.6 -2.7 -0.7
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs -0.6 -1.2 -2.0 -2.7 -0.9
lb LB -0.5 -1.7 -1.7 -2.2 -0.9
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -1.4 -1.0 -2.3 -1.9 -0.6
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -1.5 -1.4 -1.3 -1.7 -1.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.6 -1.1 -3.6 -1.2 -0.5
carbon source ferulate (C); with MOPS -2.3 -1.1 -1.1 -2.0 -0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -0.9 -2.4 -2.0 -0.5 -0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.1 -1.4 -1.6 -2.4 -0.7
pH Growth at pH7 and (C) D-Glucose -0.6 +0.0 -4.2 -1.2 -0.1
lb LB -0.2 -1.8 -1.9 -1.3 -1.0
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -0.8 -1.1 -2.4 -1.0 -0.8
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.2 -1.7 -1.3 -1.8 -1.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.4 -1.4 -2.7 -1.1 -0.2
carbon source M9 minimal media_noCarbon with Trisodium citrate 10 mM; 30C; then outgrowth in LB -0.4 -1.1 -2.3 -1.3 -0.7
pH Growth at pH6 and (C) D-Glucose +0.0 -0.4 -4.0 -1.3 -0.1
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -2.4 -0.5 -2.0 -0.8 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -4.2 -0.1 -0.5 +1.2 -0.4
stress R2A with Fusaric 480 ug/mL +0.1 +0.5 -2.6 +0.8 -2.0
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X +1.2 +1.5 +1.2 +1.2 +0.9
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay +1.5 +2.2 +3.1 +1.2 +2.2
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