Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4247 and PP_4248 are separated by 74 nucleotidesPP_4248 and PP_4249 are separated by 114 nucleotidesPP_4249 and PP_4250 are separated by 50 nucleotidesPP_4250 and PP_4251 are separated by 13 nucleotides PP_4247: PP_4247 - Exonuclease, at 4,834,226 to 4,834,777 _4247 PP_4248: PP_4248 - conserved protein of unknown function, UPF0345 family, at 4,834,852 to 4,835,136 _4248 PP_4249: PP_4249 - conserved protein of unknown function, at 4,835,251 to 4,835,943 _4249 PP_4250: PP_4250 - cytochrome c oxidase subunit I, cbb3-type, at 4,835,994 to 4,837,418 _4250 PP_4251: PP_4251 - cytochrome c oxidase subunit, cbb3-type, at 4,837,432 to 4,838,040 _4251
Group Condition PP_4247 PP_4248 PP_4249 PP_4250 PP_4251
pH Growth at pH7 and (C) D-Glucose -4.1 N.D. -0.4 -0.2 -0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -2.6 N.D. +0.1 -0.6 -1.8
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs -1.8 N.D. -0.3 -1.3 -1.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.8 N.D. -0.5 -0.4 -2.6
stress Geraniol 1 vol% -0.2 N.D. -0.5 -1.9 -1.4
pH Growth at pH7 and (C) Trisodium citrate -3.8 N.D. -1.2 -0.2 +1.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.1 N.D. -0.0 -0.8 -2.7
stress R2A with Fusaric 120 ug/mL -2.6 N.D. -0.3 -0.5 -0.2
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.3 N.D. -0.5 -0.5 -1.1
stress Isobutyl alcohol 0.6 vol% -2.4 N.D. -0.1 -0.2 -0.6
pH Growth at pH8 and (C) Trisodium citrate -2.2 N.D. +0.1 +0.0 -0.9
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -1.1 N.D. +0.1 -0.5 -1.5
nitrogen source D-2-Aminobutyric (N) -2.3 N.D. -0.4 +0.3 -0.5
carbon source D-Glucose (C) +0.1 N.D. +0.4 -0.4 -2.9
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -0.9 N.D. +0.1 -0.5 -1.5
carbon source D-Glucose (C); with MOPS -2.1 N.D. -0.1 +0.1 -0.7
stress R2A with Fusaric 240 ug/mL -2.6 N.D. +0.1 -0.3 +0.1
phage P. putida PUT 7 MOI 1 -2.1 N.D. -0.1 +0.2 -0.7
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.1 N.D. +0.0 -1.3 -1.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.6 N.D. +0.2 -1.0 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -2.5 N.D. +0.2 -0.1 -0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -2.4 N.D. +0.2 -0.4 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -3.0 N.D. -0.3 +0.3 +0.6
nitrogen source Urea (N) -2.0 N.D. +0.1 +0.3 -0.7
nitrogen source nitrate (N) -2.0 N.D. -1.1 +0.1 +0.7
stress R2A with Polymyxin B sulfate 0.001 mg/ml -1.6 N.D. +0.1 -0.8 +0.9
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.6 N.D. -0.5 -1.1 +0.9
temperature shift Temperature shift 30_to_25; with MOPS +1.2 N.D. -0.4 +0.2 -1.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +1.1 N.D. +0.2 -0.7 -1.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +1.9 N.D. +0.0 -0.9 -0.3
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