Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4235 and PP_4236 overlap by 1 nucleotidesPP_4236 and PP_4237 are separated by 1 nucleotidesPP_4237 and PP_4238 overlap by 1 nucleotidesPP_4238 and PP_5665 are separated by 392 nucleotides PP_4235: PP_4235 - thiol/disulfide interchange protein 2, at 4,808,446 to 4,810,161 _4235 PP_4236: PP_4236 - putative Thiol:disulfide interchange protein dsbE, at 4,810,161 to 4,810,997 _4236 PP_4237: PP_4237 - periplasmic protein disulfide isomerase, at 4,810,999 to 4,811,757 _4237 PP_4238: PP_4238 - conserved protein of unknown function, at 4,811,757 to 4,812,080 _4238 PP_5665: PP_5665 - putative microcin B17-like protein, McbA family, at 4,812,473 to 4,812,715 _5665
Group Condition PP_4235 PP_4236 PP_4237 PP_4238 PP_5665
nitrogen source NAG (N); with MOPS +0.1 -0.3 +0.0 -7.5 N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.5 -0.4 -0.0 -2.8 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days -0.3 -2.1 -0.0 -0.2 N.D.
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.3 -0.6 -0.6 -1.3 N.D.
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.5 -1.0 -1.2 +0.3 N.D.
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.0 -0.6 -0.2 -1.4 N.D.
pH Valerolactone (C) pH 6.5 +0.1 -0.2 -0.2 -2.0 N.D.
carbon source Valerolactone (C) -0.6 -1.3 +0.3 -0.7 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.6 -0.8 -0.2 -0.6 N.D.
stress 3-methyl-3-butenol (commercial) 0.25 vol% -0.4 -1.7 +0.1 -0.1 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.6 -0.7 -0.2 -0.6 N.D.
temperature Growth at 25C +0.0 -0.1 -0.1 -1.8 N.D.
stress 3-methyl-3-butenol (pure) 0.25 vol% -0.7 -1.6 +0.1 +0.3 N.D.
pH Growth at pH6 and (C) D-Glucose -0.0 +0.8 -0.3 -2.2 N.D.
carbon source 4-Aminobutyric 10 mM (C) -0.2 +0.1 +0.1 -1.8 N.D.
carbon source 3-methyl-1-butanol 0.25 vol% (C) -0.6 -1.2 +0.6 -0.2 N.D.
pH pH 7 -0.0 -0.2 +0.3 -1.5 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.7 -1.0 +0.1 +0.3 N.D.
carbon source carbon source 3-O-methyl-glucose 10 mM -0.0 -0.0 +0.5 -1.7 N.D.
nitrogen source Propandiamine (N) -0.7 -1.0 +0.2 +0.4 N.D.
pH pH 6 -0.2 -0.2 +0.6 -1.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.5 -1.0 +0.1 +0.6 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 -1.2 +1.6 -0.6 N.D.
carbon source Valerolactone (C) -0.3 -1.0 +0.1 +0.8 N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.2 -1.6 +0.8 +1.4 N.D.
phage P. putida Sci Big MOI 0.1 +0.2 +0.9 +0.7 -1.0 N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs +0.2 -1.0 +0.7 +1.0 N.D.
nitrogen source 2,6-diaminopimelic (N) -1.0 +1.0 +0.2 +0.9 N.D.
stress MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL +0.7 +0.9 +0.4 -0.5 N.D.
phage P. putida Missley MOI 0.1 -0.3 +0.9 +0.3 +0.7 N.D.
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