Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4230 and PP_4231 overlap by 4 nucleotidesPP_4231 and PP_4232 are separated by 3 nucleotidesPP_4232 and PP_4233 are separated by 2 nucleotidesPP_4233 and PP_4234 overlap by 8 nucleotides PP_4230: PP_4230 - conserved exported protein of unknown function, at 4,801,779 to 4,802,345 _4230 PP_4231: PP_4231 - putative Xanthine dehydrogenase accessory factor, at 4,802,342 to 4,803,313 _4231 PP_4232: PP_4232 - Cytochrome c family protein, at 4,803,317 to 4,804,528 _4232 PP_4233: PP_4233 - putative Oxidoreductase, small subunit, at 4,804,531 to 4,805,067 _4233 PP_4234: PP_4234 - Aldehyde oxidase and xanthine dehydrogenase, at 4,805,060 to 4,807,891 _4234
Group Condition PP_4230 PP_4231 PP_4232 PP_4233 PP_4234
phage P. putida Sci Big MOI 0.1 -0.7 -0.7 -0.2 -0.6 -0.4
carbon source Vanillin (C) +0.2 -2.5 -0.1 -0.1 -0.1
carbon source Vanillin (C) -0.1 -1.8 -0.2 -0.2 -0.2
carbon source Vanillin (C) -0.1 -2.5 +0.2 +0.2 +0.1
nitrogen source Ammonium chloride (N) -0.5 -0.4 -0.1 -0.7 -0.2
nitrogen source D-2-Aminobutyric (N) -0.6 +0.1 -0.2 -1.1 -0.1
carbon source Vanillin (C) -0.4 -1.9 +0.0 +0.2 +0.2
pH Valerolactone (C) pH 6.5 -0.2 -0.5 -0.3 -0.6 -0.2
carbon source Valerolactone (C) -0.8 -0.8 +0.0 +0.4 -0.2
phage P. putida Sci small MOI 0.1 -1.2 -0.2 -0.3 +0.2 +0.1
temperature shift Temperature shift 30_to_21; with MOPS +0.2 +0.0 +0.0 -1.1 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.6 -0.2 -0.4 +0.2 -0.2
pH Growth at pH6 and (C) D-Glucose -0.9 +0.3 -0.3 -0.1 -0.1
nitrogen source 2,6-diaminopimelic (N) -0.4 +0.5 -0.3 -0.6 -0.3
carbon source L-Leucine (C) -0.4 +0.2 +0.4 -1.1 -0.0
carbon source carbon source 4-vinylphenol 0.01 mM -0.2 +0.7 -0.1 -0.9 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.3 -0.8 -0.2 +0.3 -0.1
phage P. putida Sci small MOI 0.1 +0.3 -0.4 -0.2 +0.9 -0.2
nitrogen source D-2-Aminobutyric (N) -0.1 +1.0 -0.0 -0.5 +0.2
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.2 +0.7 +0.2 +0.2 -0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs +1.0 -0.2 +0.1 -0.4 +0.0
stress Growth with Chloride 500 mM; with MOPS +0.4 +0.3 -0.1 +0.6 -0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.4 +0.1 +0.2 +1.0 +0.1
nitrogen source Urea (N) +0.5 -0.3 +0.1 +0.5 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs +0.9 -0.3 +0.3 +0.3 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.5 -0.6 +0.5 +0.7 +0.1
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 +0.3 +0.0 +0.9 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs +0.8 +0.4 +0.3 +0.4 -0.1
nitrogen source NAG (N); with MOPS +0.4 +0.6 +0.2 +0.7 -0.0
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 +0.4 +0.1 +1.1 +0.6
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