Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4200 and PP_4201 are separated by 75 nucleotidesPP_4201 and PP_4202 are separated by 0 nucleotidesPP_4202 and PP_4203 are separated by 535 nucleotidesPP_4203 and PP_4204 are separated by 71 nucleotides PP_4200: PP_4200 - conserved exported protein of unknown function, at 4,745,185 to 4,746,006 _4200 PP_4201: PP_4201 - Electron transfer flavoprotein subunit alpha, at 4,746,082 to 4,747,011 _4201 PP_4202: PP_4202 - Electron transfer flavoprotein subunit beta, at 4,747,012 to 4,747,761 _4202 PP_4203: PP_4203 - Electron transfer flavoprotein-ubiquinone oxidoreductase, at 4,748,297 to 4,749,979 _4203 PP_4204: PP_4204 - antitoxin of the type II toxin-antitoxin MqsRA system and DNA-binding transcriptional repressor, at 4,750,051 to 4,750,452 _4204
Group Condition PP_4200 PP_4201 PP_4202 PP_4203 PP_4204
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X -0.2 N.D. N.D. N.D. -2.3
r2a control with 0.2x vogels R2A control with 0.2X Vogels +0.1 N.D. N.D. N.D. -2.4
micoeukaryotes Tetrahymena sp., Mixed culture-1 -0.2 N.D. N.D. N.D. -2.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.1 N.D. N.D. N.D. -2.3
stress R2A with Fusaric 240 ug/mL +0.5 N.D. N.D. N.D. -2.5
stress R2A with Fusaric 240 ug/mL +0.2 N.D. N.D. N.D. -2.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.3 N.D. N.D. N.D. -2.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.4 N.D. N.D. N.D. -1.5
micoeukaryotes Acanthamoeba sp., muxed culture-2 -0.3 N.D. N.D. N.D. -1.5
r2a control with 0.2x vogels R2A control with 0.2X Vogels -0.1 N.D. N.D. N.D. -1.7
nitrogen source nitrogen source nitrite 0.5mM -0.1 N.D. N.D. N.D. -1.7
r2a control with 0.2% methanol R2A control with 0.2% methanol +0.3 N.D. N.D. N.D. -2.0
stress R2A with Fusaric 480 ug/mL +0.2 N.D. N.D. N.D. -1.8
stress R2A with Fusaric 480 ug/mL +0.1 N.D. N.D. N.D. -1.7
phage P. put South MOI 10 +0.3 N.D. N.D. N.D. -1.8
stress R2A with Fusaric 240 ug/mL +0.2 N.D. N.D. N.D. -1.7
r2a control with 0.2% methanol R2A control with 0.2% methanol +0.2 N.D. N.D. N.D. -1.7
lb outgrowth control more dilute start LB outgrowth control more dilute start +0.2 N.D. N.D. N.D. -1.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs +0.2 N.D. N.D. N.D. -1.5
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.5 N.D. N.D. N.D. -1.8
r2a control with 0.2% methanol R2A control with 0.2% methanol +0.3 N.D. N.D. N.D. -1.5
nitrogen source nitrate (N) +0.6 N.D. N.D. N.D. -1.8
carbon source ferulate (C); with MOPS +0.4 N.D. N.D. N.D. -1.5
stress MOPS minimal media_Glucose20mM with Carbenicillin 100 .g/mL +0.6 N.D. N.D. N.D. -1.2
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay -0.2 N.D. N.D. N.D. +1.6
nitrogen source L-Methionine (N) -0.2 N.D. N.D. N.D. +1.7
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO. +0.2 N.D. N.D. N.D. +1.5
carbon source Hexanoic (C) +0.4 N.D. N.D. N.D. +1.4
carbon source 1,3-Hexanediol (C) +0.4 N.D. N.D. N.D. +1.7
carbon source L-Alanine (C) +0.1 N.D. N.D. N.D. +2.0
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