Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4199 and PP_4200 are separated by 9 nucleotidesPP_4200 and PP_4201 are separated by 75 nucleotidesPP_4201 and PP_4202 are separated by 0 nucleotidesPP_4202 and PP_4203 are separated by 535 nucleotides PP_4199: PP_4199 - putative Lipoprotein, at 4,744,822 to 4,745,175 _4199 PP_4200: PP_4200 - conserved exported protein of unknown function, at 4,745,185 to 4,746,006 _4200 PP_4201: PP_4201 - Electron transfer flavoprotein subunit alpha, at 4,746,082 to 4,747,011 _4201 PP_4202: PP_4202 - Electron transfer flavoprotein subunit beta, at 4,747,012 to 4,747,761 _4202 PP_4203: PP_4203 - Electron transfer flavoprotein-ubiquinone oxidoreductase, at 4,748,297 to 4,749,979 _4203
Group Condition PP_4199 PP_4200 PP_4201 PP_4202 PP_4203
nitrogen source nitrogen source nitrite 1mM -5.5 -0.2 N.D. N.D. N.D.
phage P. put South MOI 1 -5.3 -0.1 N.D. N.D. N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -5.1 -0.3 N.D. N.D. N.D.
phage P. put South MOI 1 -5.2 -0.0 N.D. N.D. N.D.
carbon source 4-Methyl-2-oxopentanoic (C) -5.0 -0.2 N.D. N.D. N.D.
nitrogen source Ammonium chloride (N) -5.1 +0.0 N.D. N.D. N.D.
phage P. putida South MOI 0.1 -4.6 -0.3 N.D. N.D. N.D.
nitrogen source NAG (N); with MOPS -5.0 +0.2 N.D. N.D. N.D.
phage P. putida South MOI 0.1 -4.5 -0.3 N.D. N.D. N.D.
temperature shift Temperature shift 30_to_21; with MOPS -4.9 +0.2 N.D. N.D. N.D.
temperature shift Temperature shift 30_to_4; with MOPS -4.8 +0.1 N.D. N.D. N.D.
carbon source D-Glucose (C) -4.9 +0.3 N.D. N.D. N.D.
pH Growth at pH7 and (C) Trisodium citrate -4.9 +0.3 N.D. N.D. N.D.
carbon source Valerolactone (C) -4.3 -0.0 N.D. N.D. N.D.
phage P. putida Missley MOI 0.1 -3.9 -0.2 N.D. N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -3.9 -0.0 N.D. N.D. N.D.
phage P. putida PUT10 MOI 10 -3.1 -0.5 N.D. N.D. N.D.
carbon source L-Phenylalanine 5 mM (C) -3.6 +0.2 N.D. N.D. N.D.
phage P. putida PUT10 MOI 1 -3.2 -0.2 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -2.4 -0.4 N.D. N.D. N.D.
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -2.7 -0.1 N.D. N.D. N.D.
reactor_pregrowth Adaptation in M9 media timecourse 4; day 2 -2.7 +0.0 N.D. N.D. N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -2.5 -0.3 N.D. N.D. N.D.
micoeukaryotes Tetrahymena sp., Mixed culture-1 -2.5 -0.2 N.D. N.D. N.D.
phage PUT8 MOI 10 -2.7 +0.2 N.D. N.D. N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -2.7 +0.2 N.D. N.D. N.D.
carbon source D-Glucose (C) with 20AA_mix_minus_Tyr -2.8 +0.3 N.D. N.D. N.D.
carbon source Hexanoic (C) -2.8 +0.5 N.D. N.D. N.D.
carbon source butyrate (C) -2.6 +0.3 N.D. N.D. N.D.
pH Growth at pH6 and (C) Trisodium citrate -2.5 +0.5 N.D. N.D. N.D.
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