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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
PP_4196 and PP_4197 are separated by 64 nucleotides
PP_4197 and PP_4198 are separated by 21 nucleotides
PP_4198 and PP_4199 overlap by 4 nucleotides
PP_4199 and PP_4200 are separated by 9 nucleotides
PP_4196: PP_4196 - conserved exported protein of unknown function, at 4,741,900 to 4,742,334
_4196
PP_4197: PP_4197 - Transcriptional regulator, GntR family, at 4,742,399 to 4,743,991
_4197
PP_4198: PP_4198 - OmpA family protein, at 4,744,013 to 4,744,825
_4198
PP_4199: PP_4199 - putative Lipoprotein, at 4,744,822 to 4,745,175
_4199
PP_4200: PP_4200 - conserved exported protein of unknown function, at 4,745,185 to 4,746,006
_4200
Group
Condition
PP
_4196
PP
_4197
PP
_4198
PP
_4199
PP
_4200
phage
P. putida Missley MOI 0.1
-0.9
-1.2
-1.1
-3.9
-0.2
phage
P. put South MOI 1
+0.3
+0.1
-1.4
-5.3
-0.1
nitrogen source
Ammonium chloride (N)
-0.4
-0.4
-0.5
-5.1
+0.0
phage
P. put South MOI 1
+0.6
+0.2
-1.9
-5.2
-0.0
nitrogen source
nitrogen source nitrite 1mM
-0.1
-0.1
-0.3
-5.5
-0.2
mixed carbon source
mixed (C)s Trisodium citrate 10 mM and 4-azido-L-phenylalanine 10 mM
-0.2
-4.4
-0.2
-1.9
+0.6
phage
P. putida South MOI 0.1
+0.6
+0.2
-1.8
-4.6
-0.3
phage
P. putida South MOI 0.1
+0.2
+0.3
-1.4
-4.5
-0.3
phage
P. putida PUT10 MOI 10
+0.6
+0.3
-3.0
-3.1
-0.5
mixed carbon source
mixed (C)s Trisodium citrate 10 mM and 4-azido-L-phenylalanine 10 mM
-0.5
-5.3
+0.3
+0.0
+0.4
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
+0.4
-0.1
-0.0
-5.1
-0.3
temperature shift
Temperature shift 30_to_21; with MOPS
+0.1
-0.1
-0.2
-4.9
+0.2
mixed carbon source
mixed (C)s Trisodium citrate 10 mM and 4-azido-L-phenylalanine 10 mM
-0.8
-4.9
+0.0
+0.6
+0.2
temperature shift
Temperature shift 30_to_25; with MOPS
-2.6
-0.0
-0.5
-1.6
+0.0
carbon source
D-Glucose (C)
+0.1
+0.0
-0.1
-4.9
+0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-2.0
+0.0
+0.1
-2.4
-0.4
nitrogen source
NAG (N); with MOPS
+0.3
-0.2
+0.2
-5.0
+0.2
phage
P. put PUT 10 MOI 10
-0.3
+0.1
-2.7
-1.4
-0.1
temperature shift
Temperature shift 30_to_4; with MOPS
+0.7
-0.1
-0.2
-4.8
+0.1
phage
P. putida PUT10 MOI 1
+0.5
+0.2
-1.4
-3.2
-0.2
phage
P. putida PUT10 MOI 10
+0.7
+0.2
-2.6
-2.4
+0.0
phage
P. put PUT 10 MOI 1
-0.1
+0.2
-1.8
-2.2
-0.1
pH
Growth at pH7 and (C) Trisodium citrate
+0.8
+0.3
-0.4
-4.9
+0.3
carbon source
Valerolactone (C)
+0.4
-0.1
+0.1
-4.3
-0.0
carbon source
4-Methyl-2-oxopentanoic (C)
+1.3
+0.5
-0.5
-5.0
-0.2
carbon source
L-Phenylalanine 5 mM (C)
-0.4
-0.1
+0.2
-3.6
+0.2
carbon source
4-Aminobutyric 10 mM (C)
-3.4
+0.1
-0.0
+0.9
-0.0
phage
P. put PUT 10 MOI 10
+0.7
+0.3
-2.4
-1.1
+0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
+1.0
+0.5
-0.3
-2.5
-0.3
nitrogen source
D-2-Aminobutyric (N)
-1.8
-0.9
+0.8
+0.4
+0.7
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