Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4196 and PP_4197 are separated by 64 nucleotidesPP_4197 and PP_4198 are separated by 21 nucleotidesPP_4198 and PP_4199 overlap by 4 nucleotidesPP_4199 and PP_4200 are separated by 9 nucleotides PP_4196: PP_4196 - conserved exported protein of unknown function, at 4,741,900 to 4,742,334 _4196 PP_4197: PP_4197 - Transcriptional regulator, GntR family, at 4,742,399 to 4,743,991 _4197 PP_4198: PP_4198 - OmpA family protein, at 4,744,013 to 4,744,825 _4198 PP_4199: PP_4199 - putative Lipoprotein, at 4,744,822 to 4,745,175 _4199 PP_4200: PP_4200 - conserved exported protein of unknown function, at 4,745,185 to 4,746,006 _4200
Group Condition PP_4196 PP_4197 PP_4198 PP_4199 PP_4200
phage P. putida Missley MOI 0.1 -0.9 -1.2 -1.1 -3.9 -0.2
phage P. put South MOI 1 +0.3 +0.1 -1.4 -5.3 -0.1
nitrogen source Ammonium chloride (N) -0.4 -0.4 -0.5 -5.1 +0.0
phage P. put South MOI 1 +0.6 +0.2 -1.9 -5.2 -0.0
nitrogen source nitrogen source nitrite 1mM -0.1 -0.1 -0.3 -5.5 -0.2
mixed carbon source mixed (C)s Trisodium citrate 10 mM and 4-azido-L-phenylalanine 10 mM -0.2 -4.4 -0.2 -1.9 +0.6
phage P. putida South MOI 0.1 +0.6 +0.2 -1.8 -4.6 -0.3
phage P. putida South MOI 0.1 +0.2 +0.3 -1.4 -4.5 -0.3
phage P. putida PUT10 MOI 10 +0.6 +0.3 -3.0 -3.1 -0.5
mixed carbon source mixed (C)s Trisodium citrate 10 mM and 4-azido-L-phenylalanine 10 mM -0.5 -5.3 +0.3 +0.0 +0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs +0.4 -0.1 -0.0 -5.1 -0.3
temperature shift Temperature shift 30_to_21; with MOPS +0.1 -0.1 -0.2 -4.9 +0.2
mixed carbon source mixed (C)s Trisodium citrate 10 mM and 4-azido-L-phenylalanine 10 mM -0.8 -4.9 +0.0 +0.6 +0.2
temperature shift Temperature shift 30_to_25; with MOPS -2.6 -0.0 -0.5 -1.6 +0.0
carbon source D-Glucose (C) +0.1 +0.0 -0.1 -4.9 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -2.0 +0.0 +0.1 -2.4 -0.4
nitrogen source NAG (N); with MOPS +0.3 -0.2 +0.2 -5.0 +0.2
phage P. put PUT 10 MOI 10 -0.3 +0.1 -2.7 -1.4 -0.1
temperature shift Temperature shift 30_to_4; with MOPS +0.7 -0.1 -0.2 -4.8 +0.1
phage P. putida PUT10 MOI 1 +0.5 +0.2 -1.4 -3.2 -0.2
phage P. putida PUT10 MOI 10 +0.7 +0.2 -2.6 -2.4 +0.0
phage P. put PUT 10 MOI 1 -0.1 +0.2 -1.8 -2.2 -0.1
pH Growth at pH7 and (C) Trisodium citrate +0.8 +0.3 -0.4 -4.9 +0.3
carbon source Valerolactone (C) +0.4 -0.1 +0.1 -4.3 -0.0
carbon source 4-Methyl-2-oxopentanoic (C) +1.3 +0.5 -0.5 -5.0 -0.2
carbon source L-Phenylalanine 5 mM (C) -0.4 -0.1 +0.2 -3.6 +0.2
carbon source 4-Aminobutyric 10 mM (C) -3.4 +0.1 -0.0 +0.9 -0.0
phage P. put PUT 10 MOI 10 +0.7 +0.3 -2.4 -1.1 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs +1.0 +0.5 -0.3 -2.5 -0.3
nitrogen source D-2-Aminobutyric (N) -1.8 -0.9 +0.8 +0.4 +0.7
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