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Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_4192 and PP_4193 overlap by 7 nucleotides
PP_4193 and PP_4194 are separated by 352 nucleotides
PP_4194 and PP_4195 are separated by 752 nucleotides
PP_4195 and PP_4196 are separated by 129 nucleotides
PP_4192: PP_4192 - succinate dehydrogenase hydrophobic membrane anchor subunit, at 4,738,373 to 4,738,741
_4192
PP_4193: PP_4193 - succinate dehydrogenase membrane b-556 subunit, at 4,738,735 to 4,739,121
_4193
PP_4194: PP_4194 - citrate synthase, at 4,739,474 to 4,740,763
_4194
PP_4195: PP_4195 - putative oxidoreductase, at 4,741,516 to 4,741,770
_4195
PP_4196: PP_4196 - conserved exported protein of unknown function, at 4,741,900 to 4,742,334
_4196
Group
Condition
PP
_4192
PP
_4193
PP
_4194
PP
_4195
PP
_4196
carbon source
4-Aminobutyric 10 mM (C)
N.D.
N.D.
N.D.
N.D.
-3.4
pH
Growth at pH8 and (C) Trisodium citrate
N.D.
N.D.
N.D.
N.D.
-2.8
temperature shift
Temperature shift 30_to_25; with MOPS
N.D.
N.D.
N.D.
N.D.
-2.6
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
N.D.
N.D.
N.D.
-2.5
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
N.D.
N.D.
N.D.
-2.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
N.D.
N.D.
N.D.
N.D.
-2.0
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
N.D.
N.D.
N.D.
N.D.
-1.9
pH
Growth at pH9 and (C) Trisodium citrate
N.D.
N.D.
N.D.
N.D.
-1.9
carbon source
D-Glucose (C) with 20AA_mix_minus_Cys
N.D.
N.D.
N.D.
N.D.
-1.9
nitrogen source
nitrogen source nitrite 1mM
N.D.
N.D.
N.D.
N.D.
-1.9
nitrogen source
Propiolactam (N)
N.D.
N.D.
N.D.
N.D.
-1.8
carbon source
D-Glucose (C)
N.D.
N.D.
N.D.
N.D.
-1.8
nitrogen source
D-2-Aminobutyric (N)
N.D.
N.D.
N.D.
N.D.
-1.8
phage
P. put South MOI 10
N.D.
N.D.
N.D.
N.D.
-1.7
phage moi 100
Time6-phageFRS
N.D.
N.D.
N.D.
N.D.
-1.7
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs
N.D.
N.D.
N.D.
N.D.
-1.6
stress
MOPS minimal media_Glucose20mM with Fluoroacetate 80 mM
N.D.
N.D.
N.D.
N.D.
-1.4
nitrogen source
nitrate (N)
N.D.
N.D.
N.D.
N.D.
-1.4
pH
Growth at pH6 and (C) D-Glucose
N.D.
N.D.
N.D.
N.D.
-1.4
stress
MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL
N.D.
N.D.
N.D.
N.D.
-1.4
pH
pH 5
N.D.
N.D.
N.D.
N.D.
-1.4
no stress control
Growth with no stress
N.D.
N.D.
N.D.
N.D.
-1.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
N.D.
N.D.
N.D.
N.D.
-1.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
N.D.
N.D.
N.D.
N.D.
+1.3
carbon source
4-Methyl-2-oxopentanoic (C)
N.D.
N.D.
N.D.
N.D.
+1.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
N.D.
N.D.
N.D.
N.D.
+1.3
carbon source
3-methyl-1-butanol 0.25 vol% (C)
N.D.
N.D.
N.D.
N.D.
+1.5
carbon source
ferulate (C); with MOPS
N.D.
N.D.
N.D.
N.D.
+1.5
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
N.D.
N.D.
N.D.
N.D.
+1.5
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
N.D.
N.D.
N.D.
N.D.
+1.7
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