Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4176 and PP_4177 are separated by 99 nucleotidesPP_4177 and PP_4178 are separated by 94 nucleotidesPP_4178 and PP_4179 are separated by 63 nucleotidesPP_4179 and PP_4180 are separated by 69 nucleotides PP_4176: PP_4176 - putative 5-oxo-L-prolinase, at 4,718,787 to 4,719,794 _4176 PP_4177: PP_4177 - conserved protein of unknown function, at 4,719,894 to 4,720,775 _4177 PP_4178: PP_4178 - Dienelactone hydrolase family protein, at 4,720,870 to 4,721,595 _4178 PP_4179: PP_4179 - Chaperone protein HtpG, at 4,721,659 to 4,723,563 _4179 PP_4180: PP_4180 - conserved protein of unknown function, at 4,723,633 to 4,724,115 _4180
Group Condition PP_4176 PP_4177 PP_4178 PP_4179 PP_4180
pH Growth at pH6 and (C) D-Glucose +0.1 +0.1 -2.2 +0.2 -2.6
carbon source L-Leucine (C) -0.1 +0.2 -0.3 -1.9 +0.2
carbon source L-Leucine (C) -0.1 -0.1 -0.2 -1.7 +0.4
nitrogen source Ammonium chloride (N) -0.1 -0.2 -0.5 +0.4 -1.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.3 +0.3 +0.0 +0.1 -1.7
nitrogen source 2,6-diaminopimelic (N) +0.0 +0.3 -1.1 -0.3 -0.3
micoeukaryotes Acanthamoeba sp., muxed culture-2 -0.3 +0.1 +0.4 -0.8 -0.7
nitrogen source Urea (N) +0.2 -0.6 -0.7 +0.2 -0.3
nitrogen source Urea (N) -0.2 +0.1 -1.1 -0.2 +0.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 +0.1 -0.9 -0.6 +0.5
nitrogen source NAG (N); with MOPS +0.3 -0.0 +0.2 +0.4 -1.8
carbon source 4-Methyl-2-oxopentanoic (C) +0.3 +0.2 +0.2 -1.3 -0.2
carbon source 1,3-Butandiol (C) -0.0 +0.3 +0.4 -1.1 -0.2
pH Growth at pH7 and (C) D-Glucose -0.4 -0.1 -0.3 -0.5 +0.7
no stress control Growth with no stress +0.1 +0.2 -1.1 -0.0 +0.6
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. +0.1 +0.1 -0.5 +0.6 -0.7
carbon source L-Leucine (C) +0.3 +0.3 +0.2 -1.3 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.2 -0.1 +0.6 +0.2 -0.9
carbon source carbon source 4-vinylphenol 0.01 mM +0.2 +0.5 -1.1 +0.3 +0.1
nitrogen source D-2-Aminobutyric (N) -0.1 +0.1 +0.1 +1.2 -1.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.1 -0.3 +0.8 +0.4 -0.4
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.2 -0.2 +0.5 -0.6 +1.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.2 -0.6 +0.4 +0.0 +0.9
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.5 -0.1 +0.5 -0.4 +0.6
stress R2A with Polymyxin B sulfate 0.001 mg/ml +0.6 +0.1 -0.3 +0.7 +0.2
carbon source Delta-Decalactone (C) +0.2 -0.3 +0.5 +0.7 +0.6
nitrogen source D-2-Aminobutyric (N) +0.1 -0.4 +0.4 +1.3 +0.4
carbon source DL-2-Aminoadipic 10 mM (C) +0.1 +0.2 +0.3 +1.1 +0.3
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.3 +0.3 -0.2 -0.2 +2.4
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay +0.3 +0.1 +0.2 +0.6 +1.0
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