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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_4176 and PP_4177 are separated by 99 nucleotides
PP_4177 and PP_4178 are separated by 94 nucleotides
PP_4178 and PP_4179 are separated by 63 nucleotides
PP_4179 and PP_4180 are separated by 69 nucleotides
PP_4176: PP_4176 - putative 5-oxo-L-prolinase, at 4,718,787 to 4,719,794
_4176
PP_4177: PP_4177 - conserved protein of unknown function, at 4,719,894 to 4,720,775
_4177
PP_4178: PP_4178 - Dienelactone hydrolase family protein, at 4,720,870 to 4,721,595
_4178
PP_4179: PP_4179 - Chaperone protein HtpG, at 4,721,659 to 4,723,563
_4179
PP_4180: PP_4180 - conserved protein of unknown function, at 4,723,633 to 4,724,115
_4180
Group
Condition
PP
_4176
PP
_4177
PP
_4178
PP
_4179
PP
_4180
pH
Growth at pH6 and (C) D-Glucose
+0.1
+0.1
-2.2
+0.2
-2.6
carbon source
L-Leucine (C)
-0.1
+0.2
-0.3
-1.9
+0.2
carbon source
L-Leucine (C)
-0.1
-0.1
-0.2
-1.7
+0.4
nitrogen source
Ammonium chloride (N)
-0.1
-0.2
-0.5
+0.4
-1.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
-0.3
+0.3
+0.0
+0.1
-1.7
nitrogen source
2,6-diaminopimelic (N)
+0.0
+0.3
-1.1
-0.3
-0.3
micoeukaryotes
Acanthamoeba sp., muxed culture-2
-0.3
+0.1
+0.4
-0.8
-0.7
nitrogen source
Urea (N)
+0.2
-0.6
-0.7
+0.2
-0.3
nitrogen source
Urea (N)
-0.2
+0.1
-1.1
-0.2
+0.4
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.1
+0.1
-0.9
-0.6
+0.5
nitrogen source
NAG (N); with MOPS
+0.3
-0.0
+0.2
+0.4
-1.8
carbon source
4-Methyl-2-oxopentanoic (C)
+0.3
+0.2
+0.2
-1.3
-0.2
carbon source
1,3-Butandiol (C)
-0.0
+0.3
+0.4
-1.1
-0.2
pH
Growth at pH7 and (C) D-Glucose
-0.4
-0.1
-0.3
-0.5
+0.7
no stress control
Growth with no stress
+0.1
+0.2
-1.1
-0.0
+0.6
reactor
24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
+0.1
+0.1
-0.5
+0.6
-0.7
carbon source
L-Leucine (C)
+0.3
+0.3
+0.2
-1.3
+0.2
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
+0.2
-0.1
+0.6
+0.2
-0.9
carbon source
carbon source 4-vinylphenol 0.01 mM
+0.2
+0.5
-1.1
+0.3
+0.1
nitrogen source
D-2-Aminobutyric (N)
-0.1
+0.1
+0.1
+1.2
-1.0
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
-0.1
-0.3
+0.8
+0.4
-0.4
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-0.2
-0.2
+0.5
-0.6
+1.0
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
+0.2
-0.6
+0.4
+0.0
+0.9
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
+0.5
-0.1
+0.5
-0.4
+0.6
stress
R2A with Polymyxin B sulfate 0.001 mg/ml
+0.6
+0.1
-0.3
+0.7
+0.2
carbon source
Delta-Decalactone (C)
+0.2
-0.3
+0.5
+0.7
+0.6
nitrogen source
D-2-Aminobutyric (N)
+0.1
-0.4
+0.4
+1.3
+0.4
carbon source
DL-2-Aminoadipic 10 mM (C)
+0.1
+0.2
+0.3
+1.1
+0.3
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.3
+0.3
-0.2
-0.2
+2.4
reactor
72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay
+0.3
+0.1
+0.2
+0.6
+1.0
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