Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4172 and PP_4173 overlap by 4 nucleotidesPP_4173 and PP_4174 are separated by 207 nucleotidesPP_4174 and PP_4175 are separated by 11 nucleotidesPP_4175 and PP_4176 are separated by 130 nucleotides PP_4172: PP_4172 - conserved exported protein of unknown function, at 4,713,883 to 4,714,773 _4172 PP_4173: PP_4173 - Sensor histidine kinase/response regulator, at 4,714,770 to 4,716,701 _4173 PP_4174: PP_4174 - 3R-3-hydroxydecanoyl acyl carrier protein (ACP) dehydratase, at 4,716,909 to 4,717,424 _4174 PP_4175: PP_4175 - 3-oxoacyl-[acyl-carrier-protein] synthase 1, at 4,717,436 to 4,718,656 _4175 PP_4176: PP_4176 - putative 5-oxo-L-prolinase, at 4,718,787 to 4,719,794 _4176
Group Condition PP_4172 PP_4173 PP_4174 PP_4175 PP_4176
carbon source Delta-Undecalactone (C) -2.4 -2.1 N.D. N.D. +0.2
carbon source Delta-Undecalactone (C) -2.3 -2.0 N.D. N.D. +0.1
phage P. putida South MOI 1 -1.3 -0.8 N.D. N.D. -0.4
phage P. put PUT 10 MOI 10 -1.4 -0.7 N.D. N.D. -0.3
pH pH 5 -1.1 -1.2 N.D. N.D. +0.3
carbon source 3-methyl-1-butanol 0.1 vol% (C) -0.9 -0.9 N.D. N.D. +0.4
carbon source L-Glutamic (C) +1.1 +1.1 N.D. N.D. -0.1
carbon source Protocatechuic Acid (C) +1.1 +1.0 N.D. N.D. +0.1
carbon source Protocatechuic Acid (C) +1.1 +1.1 N.D. N.D. -0.0
carbon source Protocatechuic Acid (C) +0.9 +1.1 N.D. N.D. +0.2
pH pH 7 +0.7 +0.8 N.D. N.D. +0.8
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. +1.1 +1.2 N.D. N.D. +0.2
nitrogen source L-Methionine (N) +0.9 +1.6 N.D. N.D. -0.0
carbon source Ethyl decanoate (C) +1.1 +1.2 N.D. N.D. +0.1
carbon source Ethyl decanoate (C) +1.2 +1.2 N.D. N.D. +0.0
nitrogen source L-Methionine (N) +1.1 +1.5 N.D. N.D. -0.1
carbon source M9 minimal media_noCarbon with Trisodium citrate 10 mM; 30C; then outgrowth in LB +1.1 +1.3 N.D. N.D. +0.2
carbon source Phenylacetic (C) +1.3 +1.3 N.D. N.D. +0.0
carbon source L-Alanine (C) +1.0 +1.5 N.D. N.D. +0.1
pH Valerolactone (C) pH 6.5 +1.2 +1.7 N.D. N.D. -0.2
carbon source Myristic (C) +1.6 +1.2 N.D. N.D. +0.1
carbon source Phenylacetic (C) +1.4 +1.4 N.D. N.D. +0.1
carbon source 3-Hydroxydecanoic (C) +1.4 +1.7 N.D. N.D. -0.0
carbon source 3-Hydroxydecanoic (C) +1.7 +1.9 N.D. N.D. -0.4
nitrogen source 2,6-diaminopimelic (N) +1.5 +1.9 N.D. N.D. +0.0
pH Valerolactone (C) pH 6.5 +1.6 +2.0 N.D. N.D. +0.0
carbon source Myristic (C) +2.0 +1.7 N.D. N.D. -0.0
nitrogen source 2,6-diaminopimelic (N) +1.8 +2.0 N.D. N.D. +0.2
carbon source Valerolactone (C) +1.8 +2.1 N.D. N.D. +0.2
carbon source Valerolactone (C) +2.0 +2.0 N.D. N.D. +0.1
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