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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_4164 and PP_4165 overlap by 4 nucleotides
PP_4165 and PP_4166 are separated by 17 nucleotides
PP_4166 and PP_4167 are separated by 162 nucleotides
PP_4167 and PP_4168 overlap by 4 nucleotides
PP_4164: PP_4164 - Alpha/beta hydrolase, at 4,707,408 to 4,708,262
_4164
PP_4165: PP_4165 - conserved protein of unknown function, at 4,708,259 to 4,709,122
_4165
PP_4166: PP_4166 - conserved protein of unknown function, at 4,709,140 to 4,709,514
_4166
PP_4167: PP_4167 - Phosphohistidine phosphatase SixA, at 4,709,677 to 4,710,135
_4167
PP_4168: PP_4168 - conserved protein of unknown function, at 4,710,132 to 4,710,479
_4168
Group
Condition
PP
_4164
PP
_4165
PP
_4166
PP
_4167
PP
_4168
carbon source
Delta-Nonalactone (C)
-4.5
-1.1
+0.5
+0.2
+0.3
phage
P. putida Sci small MOI 0.1
-0.9
-0.9
-1.4
-0.2
+0.1
carbon source
Delta-Decalactone (C)
-1.8
-0.9
+0.0
-0.1
-0.6
nitrogen source
Urea (N); with MOPS
-0.4
-0.5
-0.9
-0.2
-1.1
phage
P. putida Sci Big MOI 0.1
-1.4
-0.4
-0.2
-0.1
-0.3
carbon source
Delta-Decalactone (C)
-1.9
-0.5
+0.4
+0.2
-0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
-0.3
+0.0
-1.0
-0.4
-0.4
carbon source
1,3-Butandiol (C)
-1.0
-0.1
-0.3
+0.2
-0.4
carbon source
ferulate (C); with MOPS
+0.2
-0.2
-0.7
+0.1
-0.9
pH
Growth at pH9 and (C) Trisodium citrate
+0.3
+0.1
-0.7
-0.2
-0.7
nitrogen source
nitrogen source nitrite 1mM
+0.1
-0.2
-0.9
+0.4
-0.5
phage
P. putida Sci small MOI 0.1
+0.2
+0.4
+0.3
-1.2
-0.5
phage
P. putida PUT 7 MOI 1
-0.4
+0.0
+0.6
-0.1
-0.9
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
+0.2
-0.3
+0.6
-0.2
-1.0
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-0.2
+0.1
+0.0
+0.7
-1.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.8
+0.2
-0.6
-0.5
+1.2
temperature shift
Temperature shift 10_to_30; with MOPS
+0.4
+0.2
-0.6
+0.3
-0.7
stress
Growth with Chloride 200 mM
+0.3
+0.4
-0.4
+0.2
-0.8
pH
Growth at pH6 and (C) Trisodium citrate
+0.1
+0.3
-1.3
+0.1
+0.5
phage
PUT 8 MOI 1
+0.3
+0.2
-0.6
+0.7
-0.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs
+0.0
+0.4
+0.5
-0.9
+0.3
nitrogen source
nitrogen source nitrite 0.5mM
+0.3
-0.6
+0.6
+0.6
-0.3
pH
pH 9
-0.2
+0.3
+0.5
-0.5
+0.6
nitrogen source
D-2-Aminobutyric (N)
-0.2
-0.5
-0.0
+0.3
+1.3
pH
Growth at pH7 and (C) Trisodium citrate
+0.8
-0.0
-0.7
+0.7
+0.0
temperature shift
Temperature shift 30_to_21; with MOPS
+0.3
+0.3
+0.6
+0.5
-0.6
carbon source
L-Leucine (C)
+0.9
-0.5
+0.2
+0.4
+0.0
pH
Valerolactone (C) pH 6.5
-0.5
+0.0
+0.2
+0.7
+0.6
nitrogen source
D-2-Aminobutyric (N)
+0.0
+0.7
-0.9
+0.6
+0.6
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.7
+0.4
+0.2
-0.2
+0.5
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