Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4134 and PP_4135 overlap by 8 nucleotidesPP_4135 and PP_4136 are separated by 11 nucleotidesPP_4136 and PP_4137 are separated by 220 nucleotidesPP_4137 and PP_4138 are separated by 103 nucleotides PP_4134: PP_4134 - conserved membrane protein of unknown function, at 4,672,046 to 4,672,408 _4134 PP_4135: PP_4135 - conserved protein of unknown function, at 4,672,401 to 4,672,748 _4135 PP_4136: PP_4136 - Transcriptional regulator, LuxR family, at 4,672,760 to 4,673,008 _4136 PP_4137: PP_4137 - putative Outer membrane siderophore receptor, at 4,673,229 to 4,675,301 _4137 PP_4138: PP_4138 - Chromate reductase, at 4,675,405 to 4,675,965 _4138
Group Condition PP_4134 PP_4135 PP_4136 PP_4137 PP_4138
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs N.D. -0.6 -4.3 +0.3 -0.4
nitrogen source nitrogen source nitrite 0.5mM N.D. -0.4 -3.6 -0.0 -0.8
carbon source Tween 20 (C) N.D. -0.4 -4.0 +0.2 +0.0
pH Growth at pH6 and (C) D-Glucose N.D. -0.3 -3.2 -0.1 -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. +0.4 -2.6 +0.1 -1.6
nitrogen source Ammonium chloride (N) N.D. -0.1 -3.1 -0.0 -0.2
temperature Growth at 25C N.D. -0.5 -3.3 -0.0 +0.5
phage P. putida South MOI 0.1 N.D. -0.7 -2.1 -0.3 +0.0
nitrogen source Urea (N) N.D. -0.3 -3.0 -0.0 +0.4
temperature shift Temperature shift 30_to_25; with MOPS N.D. -0.0 -2.9 +0.1 -0.1
stress R2A with Fusaric 480 ug/mL N.D. -0.1 -2.3 +0.1 -0.6
pH Growth at pH6 and (C) Trisodium citrate N.D. -0.4 -2.9 +0.2 +0.5
nitrogen source Propandiamine (N) N.D. +0.0 -2.3 -0.1 -0.2
carbon source Soil Extract with D-Glucose 10 mM; 30C; then outgrowth in LB N.D. +0.1 -2.6 +0.0 -0.1
no phage control no phage control N.D. +0.1 -2.5 +0.1 -0.2
nitrogen source NAG (N); with MOPS N.D. +0.0 -3.1 -0.2 +0.7
stress R2A with Polymyxin B sulfate 0.001 mg/ml N.D. -0.0 -2.1 +0.2 -0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs N.D. -0.4 -2.0 +0.1 +0.1
phage P. putida Sci small MOI 0.1 N.D. +0.4 -2.6 -0.2 +0.6
carbon source ferulate (C); with MOPS N.D. +0.2 +0.7 +0.0 -1.9
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +1.2 -1.1 +0.1 -0.7
carbon source acetate (C) N.D. -1.1 +1.9 +0.0 -0.6
nitrogen source nitrite (N) N.D. -0.4 +2.1 +0.0 -0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days N.D. -0.7 +1.6 +0.0 +0.4
carbon source D-Glucose (C); with MOPS N.D. -0.2 +2.5 -0.2 -0.2
nitrogen source D-2-Aminobutyric (N) N.D. -0.1 +2.8 -0.2 -0.4
temperature Growth at 30C; with MOPS N.D. -0.2 +1.9 +0.1 +0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs N.D. +0.2 +2.2 -0.1 +0.1
micoeukaryotes Tetrahymena sp., Mixed culture-2 N.D. -0.2 +1.7 -0.0 +0.9
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. +0.1 +2.9 -0.1 +0.1
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