Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4128 and PP_4129 overlap by 4 nucleotidesPP_4129 and PP_4130 are separated by 40 nucleotidesPP_4130 and PP_4131 are separated by 7 nucleotidesPP_4131 and PP_4132 are separated by 90 nucleotides PP_4128: PP_4128 - NADH-quinone oxidoreductase subunit K, at 4,665,930 to 4,666,238 _4128 PP_4129: PP_4129 - NADH:ubiquinone oxidoreductase, membrane subunit L, at 4,666,235 to 4,668,088 _4129 PP_4130: PP_4130 - NADH:ubiquinone oxidoreductase, membrane subunit M, at 4,668,129 to 4,669,661 _4130 PP_4131: PP_4131 - NADH-quinone oxidoreductase subunit N, at 4,669,669 to 4,671,138 _4131 PP_4132: PP_4132 - conserved protein of unknown function, at 4,671,229 to 4,671,492 _4132
Group Condition PP_4128 PP_4129 PP_4130 PP_4131 PP_4132
carbon source Levulinic Acid (C) N.D. -4.4 -2.9 -3.9 +0.1
carbon source Delta-Undecalactone (C) N.D. -3.5 -3.1 -4.2 +0.1
carbon source 3-methyl-1-butanol (C) N.D. -4.1 -2.5 -3.8 -0.0
carbon source Delta-Undecalactone (C) N.D. -3.4 -3.0 -3.8 -0.0
carbon source Levulinic Acid (C) N.D. -2.7 -3.2 -3.8 -0.1
carbon source 4-Methyl-2-oxopentanoic (C) N.D. -3.6 -2.2 -3.2 -0.1
carbon source Delta-Decalactone (C) N.D. -3.2 -2.1 -3.8 -0.1
carbon source 3-methyl-1-butanol (C) N.D. -3.0 -2.7 -3.5 +0.1
carbon source Vanillin (C) N.D. -3.3 -2.4 -3.2 +0.1
carbon source Vanillin (C) N.D. -2.8 -2.7 -3.4 +0.3
carbon source Delta-Dodecalactone (C) N.D. -2.8 -2.5 -2.9 -0.1
carbon source 3-Hydroxypropionate (C) N.D. -2.7 -3.0 -2.7 +0.1
carbon source Delta-Dodecalactone (C) N.D. -3.2 -1.8 -3.4 +0.1
carbon source 1,4-Butanediol (C) N.D. -3.1 -1.5 -3.4 -0.2
carbon source 4-Methyl-2-oxopentanoic (C) N.D. -2.7 -2.3 -3.0 -0.0
carbon source 2-methyl-1-butanol (C) N.D. -2.6 -2.9 -2.2 -0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days N.D. -3.0 -2.7 -2.7 +0.5
pH Growth at pH6 and (C) D-Glucose N.D. -2.5 -1.8 -2.5 -1.0
carbon source 4-Methyl-2-oxopentanoic (C) N.D. -3.6 -1.8 -2.3 -0.1
carbon source 3-Hydroxypropionate (C) N.D. -3.2 -1.6 -2.7 +0.0
carbon source L-Lysine (C) N.D. -2.9 -2.3 -2.3 +0.1
pH Growth at pH9 and (C) Trisodium citrate N.D. -3.4 -1.7 -2.7 +0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days N.D. -2.3 -2.6 -2.6 +0.2
micoeukaryotes Dictyostellum sp.mixed culture-2 N.D. +2.9 +2.2 +2.1 -0.3
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay N.D. +2.6 +1.8 +2.7 +0.4
reactor 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO. N.D. +3.4 +2.2 +3.0 -0.1
micoeukaryotes Acanthamoeba sp., muxed culture-2 N.D. +2.7 +2.5 +2.7 +0.5
micoeukaryotes C.elegans, mixed culture-2 N.D. +3.5 +3.3 +3.4 -0.4
nitrogen source DL-2-Aminobutyric (N) N.D. +3.9 +3.8 +3.9 +0.2
nitrogen source DL-2-Aminobutyric (N) N.D. +4.2 +3.8 +4.0 +0.3
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