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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_4102 and PP_4103 are separated by 128 nucleotides
PP_4103 and PP_4104 are separated by 180 nucleotides
PP_4104 and PP_4105 are separated by 87 nucleotides
PP_4105 and PP_4106 are separated by 57 nucleotides
PP_4102: PP_4102 - conserved protein of unknown function, at 4,636,770 to 4,637,156
_4102
PP_4103: PP_4103 - Low-affinity inorganic phosphate transporter, at 4,637,285 to 4,638,901
_4103
PP_4104: PP_4104 - Glutathione S-transferase family protein, at 4,639,082 to 4,639,777
_4104
PP_4105: PP_4105 - conserved protein of unknown function, at 4,639,865 to 4,640,362
_4105
PP_4106: PP_4106 - gamma-aminobutyrate permease, at 4,640,420 to 4,641,802
_4106
Group
Condition
PP
_4102
PP
_4103
PP
_4104
PP
_4105
PP
_4106
phage
PUT8 MOI 0.1
-0.6
-0.2
-1.1
-0.5
-0.4
nitrogen source
D-2-Aminobutyric (N)
-1.5
-0.4
-0.5
-0.0
-0.3
nitrogen source
NAG (N); with MOPS
-1.2
+0.1
-1.0
-0.0
-0.1
nitrogen source
Propiolactam (N)
-1.1
-0.1
-0.4
-0.2
-0.2
dilutelb
LB 0.1x with 55.5 mM D-Glucose
-0.8
-0.2
-0.6
-0.2
-0.1
carbon source
Delta-Decalactone (C)
-0.9
-0.3
-0.2
-0.3
-0.1
pH
Valerolactone (C) pH 6.5
-1.7
-0.2
-0.3
-0.1
+0.5
carbon source
Valerolactone (C)
-0.9
-0.1
-0.7
+0.3
-0.2
nitrogen source
L-Pipecolic Acid (N)
-0.1
+0.1
-0.5
+0.2
-1.3
carbon source
acetate (C)
-1.1
+0.0
-0.6
-0.2
+0.3
carbon source
D-Glucose (C)
-1.2
+0.0
-0.3
-0.2
+0.2
nitrogen source
L-Pipecolic Acid (N)
+0.1
+0.1
+0.1
-0.1
-1.5
pH
pH 7
+0.5
-0.4
-0.6
-0.6
-0.1
stress
R2A with Polymyxin B sulfate 0.001 mg/ml
-1.5
+0.1
+0.0
+0.3
-0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
-0.7
-0.0
-0.7
+0.2
+0.2
pH
Growth at pH7 and (C) Trisodium citrate
-0.8
+0.0
-0.7
+0.6
+0.1
temperature shift
Temperature shift 30_to_21; with MOPS
-1.4
-0.1
+0.1
+0.5
+0.1
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-1.0
-0.0
+0.9
-0.1
-0.4
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
+0.5
-0.3
-0.6
+0.3
-0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.9
+0.1
+0.2
+0.4
+0.1
pH
pH 6
+1.1
-0.2
-0.2
-0.3
-0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
+1.1
+0.1
-0.2
-0.5
-0.1
stress
Growth with Chloride 500 mM; with MOPS
-0.8
+0.1
+0.6
+0.6
-0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
+0.9
+0.2
+0.2
-0.0
-0.6
pH
Growth at pH6 and (C) Trisodium citrate
+0.6
-0.3
+0.6
-0.2
+0.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.3
-0.2
+0.6
+0.7
-0.1
carbon source
carbon source D-Glucose 55.5 mM
+1.2
-0.3
-0.2
+0.2
-0.0
carbon source
carbon source 4-vinylphenol 0.01 mM
+0.9
-0.2
-0.0
+0.6
+0.0
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
+0.9
+0.0
-0.1
+0.8
-0.1
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+1.4
-0.1
+0.9
-0.3
-0.1
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