Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4102 and PP_4103 are separated by 128 nucleotidesPP_4103 and PP_4104 are separated by 180 nucleotidesPP_4104 and PP_4105 are separated by 87 nucleotidesPP_4105 and PP_4106 are separated by 57 nucleotides PP_4102: PP_4102 - conserved protein of unknown function, at 4,636,770 to 4,637,156 _4102 PP_4103: PP_4103 - Low-affinity inorganic phosphate transporter, at 4,637,285 to 4,638,901 _4103 PP_4104: PP_4104 - Glutathione S-transferase family protein, at 4,639,082 to 4,639,777 _4104 PP_4105: PP_4105 - conserved protein of unknown function, at 4,639,865 to 4,640,362 _4105 PP_4106: PP_4106 - gamma-aminobutyrate permease, at 4,640,420 to 4,641,802 _4106
Group Condition PP_4102 PP_4103 PP_4104 PP_4105 PP_4106
phage PUT8 MOI 0.1 -0.6 -0.2 -1.1 -0.5 -0.4
nitrogen source D-2-Aminobutyric (N) -1.5 -0.4 -0.5 -0.0 -0.3
nitrogen source NAG (N); with MOPS -1.2 +0.1 -1.0 -0.0 -0.1
nitrogen source Propiolactam (N) -1.1 -0.1 -0.4 -0.2 -0.2
dilutelb LB 0.1x with 55.5 mM D-Glucose -0.8 -0.2 -0.6 -0.2 -0.1
carbon source Delta-Decalactone (C) -0.9 -0.3 -0.2 -0.3 -0.1
pH Valerolactone (C) pH 6.5 -1.7 -0.2 -0.3 -0.1 +0.5
carbon source Valerolactone (C) -0.9 -0.1 -0.7 +0.3 -0.2
nitrogen source L-Pipecolic Acid (N) -0.1 +0.1 -0.5 +0.2 -1.3
carbon source acetate (C) -1.1 +0.0 -0.6 -0.2 +0.3
carbon source D-Glucose (C) -1.2 +0.0 -0.3 -0.2 +0.2
nitrogen source L-Pipecolic Acid (N) +0.1 +0.1 +0.1 -0.1 -1.5
pH pH 7 +0.5 -0.4 -0.6 -0.6 -0.1
stress R2A with Polymyxin B sulfate 0.001 mg/ml -1.5 +0.1 +0.0 +0.3 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.7 -0.0 -0.7 +0.2 +0.2
pH Growth at pH7 and (C) Trisodium citrate -0.8 +0.0 -0.7 +0.6 +0.1
temperature shift Temperature shift 30_to_21; with MOPS -1.4 -0.1 +0.1 +0.5 +0.1
stress R2A with Polymyxin B sulfate 0.002 mg/ml -1.0 -0.0 +0.9 -0.1 -0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs +0.5 -0.3 -0.6 +0.3 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.9 +0.1 +0.2 +0.4 +0.1
pH pH 6 +1.1 -0.2 -0.2 -0.3 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +1.1 +0.1 -0.2 -0.5 -0.1
stress Growth with Chloride 500 mM; with MOPS -0.8 +0.1 +0.6 +0.6 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.9 +0.2 +0.2 -0.0 -0.6
pH Growth at pH6 and (C) Trisodium citrate +0.6 -0.3 +0.6 -0.2 +0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.3 -0.2 +0.6 +0.7 -0.1
carbon source carbon source D-Glucose 55.5 mM +1.2 -0.3 -0.2 +0.2 -0.0
carbon source carbon source 4-vinylphenol 0.01 mM +0.9 -0.2 -0.0 +0.6 +0.0
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.9 +0.0 -0.1 +0.8 -0.1
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.4 -0.1 +0.9 -0.3 -0.1
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