Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4061 and PP_4063 are separated by 214 nucleotidesPP_4063 and PP_4064 are separated by 321 nucleotidesPP_4064 and PP_4065 are separated by 19 nucleotidesPP_4065 and PP_4066 are separated by 3 nucleotides PP_4061: PP_4061 - conserved protein of unknown function, at 4,585,756 to 4,585,917 _4061 PP_4063: PP_4063 - putative Long-chain-fatty-acid-CoA ligase, at 4,586,132 to 4,587,814 _4063 PP_4064: PP_4064 - isovaleryl-CoA dehydrogenase, at 4,588,136 to 4,589,299 _4064 PP_4065: PP_4065 - methylcrotonyl-CoA carboxylase biotin-containing subunit beta, at 4,589,319 to 4,590,926 _4065 PP_4066: PP_4066 - methylglutaconyl-CoA hydratase, at 4,590,930 to 4,591,745 _4066
Group Condition PP_4061 PP_4063 PP_4064 PP_4065 PP_4066
carbon source 3-methyl-1-butanol (C) N.D. -2.1 -5.0 -6.3 -5.5
carbon source 3-methyl-1-butanol 0.1 vol% (C) N.D. -3.3 -5.1 -6.3 -4.1
carbon source 3-methyl-1-butanol 0.25 vol% (C) N.D. -3.6 -4.3 -5.8 -4.2
carbon source 3-methyl-3-buten-1-ol 0.1 vol% (C) N.D. -1.8 -4.1 -6.3 -4.4
carbon source 4-Methyl-2-oxopentanoic (C) N.D. -0.0 -5.3 -5.1 -5.9
carbon source 3-methyl-1-butanol (C) N.D. -2.5 -4.2 -5.5 -3.9
carbon source 4-Methyl-2-oxopentanoic (C) N.D. -0.3 -5.1 -4.8 -5.6
carbon source 4-Methyl-2-oxopentanoic (C) N.D. -0.0 -4.6 -5.0 -5.7
carbon source 4-Methyl-2-oxopentanoic (C) N.D. -0.2 -5.2 -5.2 -4.7
carbon source 3-methyl-3-buten-1-ol 0.25 vol% (C) N.D. -1.9 -4.0 -5.8 -3.0
carbon source 3-methyl-3-butenol (C) N.D. -1.0 -4.2 -4.6 -4.7
carbon source 3-methyl-3-butenol (C) N.D. -0.9 -4.0 -3.9 -4.9
carbon source L-Leucine (C) N.D. +0.2 -3.3 -4.5 -3.0
carbon source L-Leucine (C) N.D. -0.2 -3.7 -3.3 -1.6
carbon source L-Valine (C) N.D. -0.1 -2.0 -3.1 -3.1
carbon source L-Valine (C) N.D. -0.0 -2.0 -2.5 -2.7
carbon source L-Valine (C) N.D. +0.0 -1.7 -2.6 -2.7
carbon source L-Leucine (C) N.D. +0.1 -2.6 -2.9 -1.5
nitrogen source L-Leucine (N) N.D. +0.0 -0.8 -2.3 -3.2
carbon source 3-Methyl-2-Oxobutanoic Acid (C) N.D. +0.0 -1.1 -2.2 -2.2
carbon source 3-Methyl-2-Oxobutanoic Acid (C) N.D. +0.1 -1.2 -1.8 -2.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs N.D. +0.0 -0.6 -1.2 -2.9
nitrogen source L-Leucine (N) N.D. +0.1 -0.9 -1.8 -1.9
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs N.D. -0.1 -0.6 -1.3 -2.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs N.D. -0.1 -0.4 -1.7 -2.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. -0.2 -0.7 -0.9 -1.8
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs N.D. +0.1 -0.5 -1.2 -1.6
toxin pUC18_phdP1_pSU18T_docP1 N.D. +0.2 -0.2 -0.9 -2.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs N.D. +0.3 -0.5 -1.4 -1.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. +0.3 -1.2 -2.1 +0.5
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