Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4049 and PP_4050 are separated by 897 nucleotidesPP_4050 and PP_4051 are separated by 14 nucleotidesPP_4051 and PP_4052 overlap by 4 nucleotidesPP_4052 and PP_4053 overlap by 4 nucleotides PP_4049: PP_4049 - type 6 secretion system protein, at 4,561,545 to 4,563,860 _4049 PP_4050: PP_4050 - glycogen synthase, at 4,564,758 to 4,566,317 _4050 PP_4051: PP_4051 - malto-oligosyltrehalose trehalohydrolase, at 4,566,332 to 4,568,074 _4051 PP_4052: PP_4052 - 4-alpha-glucanotransferase, at 4,568,071 to 4,570,140 _4052 PP_4053: PP_4053 - maltooligosyl trehalose synthase, at 4,570,137 to 4,572,911 _4053
Group Condition PP_4049 PP_4050 PP_4051 PP_4052 PP_4053
micoeukaryotes C.elegans, mixed culture-1 +0.1 -2.5 -0.3 -0.5 -0.3
stress Growth with Chloride 500 mM +0.1 -0.8 -0.6 -0.5 -1.0
micoeukaryotes Acanthamoeba sp., muxed culture-2 +0.1 -2.0 -0.2 -0.4 -0.1
stress Growth with Chloride 500 mM -0.0 -0.9 -0.4 -0.4 -0.5
micoeukaryotes C.elegans, mixed culture-2 +0.2 -1.5 -0.1 -0.5 -0.1
temperature Growth at 10C -0.1 -1.3 -0.2 -0.3 -0.2
temperature Growth at 10C; with MOPS +0.1 -1.3 -0.2 -0.4 -0.1
phage P. putida South MOI 10 -0.3 -0.3 -0.4 -0.4 -0.4
micoeukaryotes Dictyostellum sp.mixed culture-2 +0.1 -1.3 -0.4 -0.2 -0.1
stress Growth with Chloride 500 mM +0.1 -0.7 -0.5 -0.2 -0.5
temperature Growth at 25C -0.3 -0.4 -0.6 -0.1 -0.3
micoeukaryotes Dictyostellum sp.mixed culture-1 +0.1 -1.4 -0.0 -0.3 +0.1
carbon source 1,3-Hexanediol (C) -0.0 -0.5 +0.1 -0.0 -1.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.2 -0.1 -0.6 -0.2 -0.6
micoeukaryotes Tetrahymena sp., Mixed culture-2 +0.2 -1.0 +0.5 -0.6 -0.2
carbon source carbon source 4-vinylphenol 0.01 mM +0.4 -0.6 +0.1 -0.3 -0.3
nitrogen source DL-2-Aminobutyric (N) +0.0 -1.0 +0.4 +0.9 -0.6
nitrogen source DL-2-Aminobutyric (N) +0.1 -0.8 +0.4 +0.9 -0.6
nitrogen source D-2-Aminobutyric (N) -0.3 -0.1 +0.5 +0.4 +0.5
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -0.2 +0.4 +0.6 +0.3 +0.3
carbon source 1,4-Butanediol (C) -0.1 +1.3 +0.1 +0.1 +0.2
carbon source L-Valine (C) -0.1 +1.1 +0.3 +0.4 +0.0
carbon source L-Glutamic (C) -0.0 +1.1 +0.4 +0.1 +0.1
carbon source L-Valine (C) +0.0 +0.8 +0.4 +0.3 +0.2
carbon source Valerolactone (C) -0.1 +0.3 +1.1 +0.5 +0.1
carbon source 4-Hydroxyvalerate (C) (40mM) +0.0 +0.8 +0.5 +0.4 +0.2
carbon source L-Valine (C) +0.0 +1.1 +0.3 +0.5 +0.1
carbon source 1,4-Butanediol (C) -0.1 +1.3 +0.4 +0.3 +0.3
carbon source 4-Hydroxyvalerate (C) (40mM) -0.0 +1.0 +0.4 +0.5 +0.2
carbon source 5-Aminovaleric 10 mM (C) +0.2 +0.8 +0.5 +0.3 +0.3
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