Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4031 and PP_4032 are separated by 146 nucleotidesPP_4032 and PP_4033 are separated by 87 nucleotidesPP_4033 and PP_4034 overlap by 4 nucleotidesPP_4034 and PP_4035 are separated by 49 nucleotides PP_4031: PP_4031 - Na(+)/H(+) antiporter NhaB, at 4,541,284 to 4,542,786 _4031 PP_4032: PP_4032 - putative Outer membrane lipoprotein Blc, at 4,542,933 to 4,543,478 _4032 PP_4033: PP_4033 - Ribonuclease Z, at 4,543,566 to 4,544,525 _4033 PP_4034: PP_4034 - N-carbamoyl-beta-alanine amidohydrolase/allantoine amidohydrolase 2, at 4,544,522 to 4,545,805 _4034 PP_4035: PP_4035 - putative PydP transporter, NCS1 nucleoside transporter family, at 4,545,855 to 4,547,345 _4035
Group Condition PP_4031 PP_4032 PP_4033 PP_4034 PP_4035
phage PUT 8 MOI 1 -0.4 -0.6 -0.1 -1.3 -0.6
nitrogen source Hydantoin (N) -0.0 +0.2 +0.0 -1.2 -1.1
stress R2A with Fusaric 240 ug/mL -0.2 +0.0 -0.6 -0.5 -0.6
nitrogen source NAG (N); with MOPS +0.2 -0.3 +0.0 -1.5 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.1 -0.3 -0.5 -0.9 -0.1
nitrogen source Hydantoin (N) -0.0 +0.0 -0.1 -0.9 -0.7
nitrogen source Hydantoin (N) -0.1 +0.4 -0.1 -0.8 -1.0
carbon source Trisodium citrate (C) -0.3 +0.1 -0.6 -0.6 -0.1
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.1 -0.6 -0.0 -0.9 +0.0
pH Growth at pH6 and (C) D-Glucose +0.2 -0.7 -0.2 -0.5 -0.0
nitrogen source Hydantoin (N) +0.1 +0.1 +0.1 -0.9 -0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.1 -0.6 +0.0 -0.8 +0.2
nitrogen source NAG (N); with MOPS -0.1 +0.4 +0.0 -0.6 -0.8
phage P. putida South MOI 10 -0.4 -0.6 +0.1 -0.3 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.0 -0.5 +0.4 -0.6 -0.2
nitrogen source D-2-Aminobutyric (N) -0.2 -0.5 -0.7 +0.5 +0.1
micoeukaryotes Acanthamoeba sp., muxed culture-2 -0.2 +0.4 -0.4 -0.5 +0.1
temperature shift Temperature shift 30_to_21; with MOPS +0.1 +0.2 +0.3 -0.8 -0.3
nitrogen source D-2-Aminobutyric (N) +0.2 +0.3 -0.8 -0.7 +0.4
temperature shift Temperature shift 30_to_21; with MOPS -0.2 +0.3 -0.2 -0.7 +0.3
carbon source L-Leucine (C) +0.3 +0.4 -0.6 -0.1 -0.2
phage P. putida Sci small MOI 0.1 +0.0 +0.4 +0.6 -1.0 -0.2
nitrogen source 2,6-diaminopimelic (N) -0.1 +0.5 +0.3 -0.6 -0.4
carbon source Heptanoic (C) -0.0 -0.8 +0.3 +0.2 +0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.0 -0.6 -0.2 +0.5 +0.5
nitrogen source 2,6-diaminopimelic (N) +0.1 -0.7 +0.4 -0.1 +0.4
carbon source Valeric (C) +0.2 -0.7 +0.3 +0.1 +0.3
pH pH 8 +0.2 -0.2 +0.4 -0.5 +0.2
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. +0.2 +0.3 +0.4 -0.6 -0.0
carbon source ferulate (C); with MOPS +0.3 +0.7 +0.2 -0.7 -0.0
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