Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4015 and PP_4016 are separated by 137 nucleotidesPP_4016 and PP_4017 are separated by 75 nucleotidesPP_4017 and PP_4018 overlap by 8 nucleotidesPP_4018 and PP_4019 are separated by 167 nucleotides PP_4015: PP_4015 - HflD-like high frequency lysogenization protein, at 4,526,191 to 4,526,817 _4015 PP_4016: PP_4016 - 5'-phosphoribosyl-4-(N-succinocarboxamide)-5- aminoimidazole lyase (adenylosuccinate lyase), at 4,526,955 to 4,528,325 _4016 PP_4017: PP_4017 - conserved protein of unknown function, at 4,528,401 to 4,529,573 _4017 PP_4018: PP_4018 - Acetyltransferase, GNAT family, at 4,529,566 to 4,529,994 _4018 PP_4019: PP_4019 - DNA topoisomerase III, at 4,530,162 to 4,532,123 _4019
Group Condition PP_4015 PP_4016 PP_4017 PP_4018 PP_4019
nitrogen source NAG (N); with MOPS -0.2 N.D. N.D. -2.1 -0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.7 N.D. N.D. -0.9 -0.4
temperature Growth at 34C -0.2 N.D. N.D. -1.4 -0.3
pH Growth at pH8 and (C) D-Glucose -0.3 N.D. N.D. -1.6 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.6 N.D. N.D. -1.1 -0.0
nitrogen source Urea (N) -0.8 N.D. N.D. -1.0 +0.0
nitrogen source Ammonium chloride (N) -0.5 N.D. N.D. -1.1 -0.1
carbon source Glutaric (C) -0.3 N.D. N.D. -1.3 -0.0
temperature Growth at 30C; with MOPS -1.3 N.D. N.D. +0.1 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.0 N.D. N.D. -1.3 -0.3
nitrogen source 2,6-diaminopimelic (N) -0.8 N.D. N.D. -0.9 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.5 N.D. N.D. -1.1 +0.1
temperature Growth at 30C; with MOPS -0.2 N.D. N.D. -1.3 +0.1
phage P. putida South MOI 0.1 +0.7 N.D. N.D. -1.7 -0.1
nitrogen source Ammonium chloride (N); with MOPS -1.0 N.D. N.D. +0.6 -0.3
temperature shift Temperature shift 30_to_25; with MOPS -0.9 N.D. N.D. +0.5 -0.3
stress Butanol 0.6 vol% +0.5 N.D. N.D. -1.1 +0.3
carbon source D-Glucose (C) -1.0 N.D. N.D. +1.0 -0.2
pH Growth at pH6 and (C) D-Glucose +0.7 N.D. N.D. -0.6 -0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -0.8 N.D. N.D. +0.7 -0.1
carbon source acetate (C) +0.4 N.D. N.D. -0.8 +0.4
nitrogen source nitrogen source nitrate 5mM -0.5 N.D. N.D. +1.1 -0.1
pH Growth at pH7 and (C) D-Glucose -0.3 N.D. N.D. +1.2 -0.4
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.9 N.D. N.D. +0.9 +0.7
temperature shift Temperature shift 10_to_30 -0.5 N.D. N.D. +1.0 +0.2
phage P. putida PUT 7 MOI 1 -0.3 N.D. N.D. +1.1 +0.1
carbon source ferulate (C); with MOPS +0.3 N.D. N.D. +1.2 -0.5
stress Growth with Chloride 200 mM; with MOPS +0.7 N.D. N.D. +0.7 -0.3
pH pH 7 +0.4 N.D. N.D. +1.1 -0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.0 N.D. N.D. +2.2 -0.2
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