Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3965 and PP_3966 overlap by 4 nucleotidesPP_3966 and PP_3967 are separated by 175 nucleotidesPP_3967 and PP_3968 are separated by 5 nucleotidesPP_3968 and PP_3969 are separated by 147 nucleotides PP_3965: PP_3965 - transposase, at 4,475,599 to 4,475,934 _3965 PP_3966: PP_3966 - transposase, at 4,475,931 to 4,476,251 _3966 PP_3967: PP_3967 - MutT/nudix family protein, at 4,476,427 to 4,476,867 _3967 PP_3968: PP_3968 - Sensor histidine kinase, at 4,476,873 to 4,477,991 _3968 PP_3969: PP_3969 - Response regulator, at 4,478,139 to 4,478,501 _3969
Group Condition PP_3965 PP_3966 PP_3967 PP_3968 PP_3969
nitrogen source nitrogen source nitrite 1mM N.D. N.D. -1.8 -0.4 -0.0
nitrogen source NAG (N); with MOPS N.D. N.D. -0.6 -0.0 -1.5
nitrogen source D-2-Aminobutyric (N) N.D. N.D. -2.0 -0.4 +0.5
carbon source 5-Aminovaleric 10 mM (C) N.D. N.D. -1.1 -0.2 -0.5
pH Growth at pH7 and (C) Trisodium citrate N.D. N.D. -0.6 -0.3 -0.8
pH Valerolactone (C) pH 6.5 N.D. N.D. -1.6 +0.1 -0.2
nitrogen source nitrogen source nitrite 1mM N.D. N.D. -1.9 +0.1 +0.1
carbon source 3-Hydroxydecanoic (C) N.D. N.D. -1.0 -0.0 -0.3
no stress control Growth with no stress N.D. N.D. -0.2 -0.1 -1.0
nitrogen source Propiolactam (N) N.D. N.D. -0.8 +0.1 -0.4
pH Growth at pH6 and (C) Trisodium citrate N.D. N.D. -1.3 +0.3 +0.1
r2a control with 0.2x vogels R2A control with 0.2X Vogels N.D. N.D. -1.0 +0.1 +0.2
nitrogen source nitrogen source nitrite 0.5mM N.D. N.D. -1.3 +0.0 +0.7
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. -0.9 +0.4 -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days N.D. N.D. +0.4 -0.3 -0.6
nitrogen source D-2-Aminobutyric (N) N.D. N.D. +0.2 -0.9 +0.3
nitrogen source Urea (N); with MOPS N.D. N.D. -0.7 +0.1 +0.4
temperature Growth at 25C N.D. N.D. -0.8 +0.3 +0.4
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. -0.9 +0.4 +0.5
carbon source carbon source 2-Deoxy-D-glucose 100 mM N.D. N.D. -0.6 +0.2 +0.5
temperature shift Temperature shift 30_to_21; with MOPS N.D. N.D. -0.7 +0.3 +0.5
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. +0.7 -0.1 -0.5
r2a control R2A control N.D. N.D. +0.5 +0.2 -0.6
phage PUT8 MOI 0.1 N.D. N.D. -0.5 -0.1 +0.7
r2a control with 0.2% methanol R2A control with 0.2% methanol N.D. N.D. -0.6 +0.2 +0.5
stress Growth with Chloride 200 mM N.D. N.D. -0.6 +0.2 +0.6
pH pH 7 N.D. N.D. -0.6 +0.3 +0.6
pH Growth at pH7 and (C) D-Glucose N.D. N.D. -0.2 -0.3 +0.8
nitrogen source 2,6-diaminopimelic (N) N.D. N.D. -0.6 +0.4 +0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs N.D. N.D. +1.0 +0.3 +0.0
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