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Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_3931 and PP_3932 are separated by 137 nucleotides
PP_3932 and PP_3933 are separated by 1 nucleotides
PP_3933 and PP_3934 are separated by 62 nucleotides
PP_3934 and PP_3935 are separated by 100 nucleotides
PP_3931: PP_3931 - putative transporter, at 4,434,874 to 4,436,703
_3931
PP_3932: PP_3932 - Diguanylate cyclase, at 4,436,841 to 4,438,265
_3932
PP_3933: PP_3933 - conserved membrane protein of unknown function, at 4,438,267 to 4,439,028
_3933
PP_3934: PP_3934 - Transcriptional regulator, LysR family, at 4,439,091 to 4,440,032
_3934
PP_3935: PP_3935 - conserved protein of unknown function, at 4,440,133 to 4,440,342
_3935
Group
Condition
PP
_3931
PP
_3932
PP
_3933
PP
_3934
PP
_3935
pH
Growth at pH6 and (C) D-Glucose
-0.6
+0.3
-0.0
-0.3
-2.0
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.7
-0.3
+0.1
-0.2
-1.5
carbon source
ferulate (C); with MOPS
+0.0
+0.0
-0.4
+0.3
-2.5
pH
Growth at pH9 and (C) Trisodium citrate
-0.4
-0.3
-0.6
+0.3
-1.1
stress
Isobutyl alcohol 0.6 vol%
-0.2
-0.2
-0.0
+0.1
-1.7
nitrogen source
Propandiamine (N)
-0.4
-0.8
-0.0
-0.4
-0.4
pH
Growth at pH6 and (C) Trisodium citrate
-0.4
-0.3
+0.0
-0.2
-1.1
nitrogen source
NAG (N); with MOPS
-0.1
-0.5
+0.3
-0.0
-1.6
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
-0.7
-0.2
+0.3
-0.4
-0.7
carbon source
D-Glucose (C)
-0.4
+0.4
+0.2
-0.3
-1.5
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
-0.9
-0.3
-0.5
+0.1
+0.3
reactor
24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
-0.2
+0.2
-0.9
-0.6
+0.2
phage
P. putida PUT 7 MOI 1
-0.3
+0.2
-0.9
-0.8
+0.5
nitrogen source
Ammonium chloride (N)
-0.0
+0.3
-0.4
+0.2
-1.3
reactor
72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
+0.2
+0.2
-0.1
-0.3
-1.2
carbon source
carbon source 2-Deoxy-D-glucose 10 mM
-0.3
+0.3
-0.9
+0.4
-0.5
carbon source
carbon source Bisabolol 45 mM
-0.3
+0.3
-0.3
+0.3
-0.7
carbon source
carbon source 4-vinylphenol 0.01 mM
-0.4
+0.1
-0.7
+0.5
-0.2
carbon source
carbon source Bisabolene 48.9 mM
-0.2
+0.4
-0.8
+0.4
-0.5
carbon source
carbon source 3-O-methyl-glucose 10 mM
-0.5
+0.3
-0.6
+0.5
-0.3
carbon source
carbon source 4-vinylphenol 0.1 mM
-0.6
+0.4
-0.6
+0.6
-0.3
carbon source
ferulate (C); with MOPS
+0.3
-0.1
-0.4
+0.4
-0.8
carbon source
carbon source D-Glucose 55.5 mM
-1.1
+0.2
-0.6
+0.3
+0.8
carbon source
carbon source 2-Deoxy-D-glucose 100 mM
-0.6
+0.6
-0.6
+0.2
+0.0
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.5
+0.3
-0.4
-0.0
-0.8
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
+0.2
-0.3
-0.7
+0.3
+0.3
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.2
+0.7
+0.2
-0.6
+0.1
nitrogen source
Propiolactam (N)
+0.3
+0.2
+0.6
+0.2
-0.9
phage
P. put South MOI 10
+0.3
+0.3
+0.3
-0.1
+1.0
nitrogen source
Propiolactam (N)
+0.2
+0.2
+0.4
+0.3
+1.3
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