Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3931 and PP_3932 are separated by 137 nucleotidesPP_3932 and PP_3933 are separated by 1 nucleotidesPP_3933 and PP_3934 are separated by 62 nucleotidesPP_3934 and PP_3935 are separated by 100 nucleotides PP_3931: PP_3931 - putative transporter, at 4,434,874 to 4,436,703 _3931 PP_3932: PP_3932 - Diguanylate cyclase, at 4,436,841 to 4,438,265 _3932 PP_3933: PP_3933 - conserved membrane protein of unknown function, at 4,438,267 to 4,439,028 _3933 PP_3934: PP_3934 - Transcriptional regulator, LysR family, at 4,439,091 to 4,440,032 _3934 PP_3935: PP_3935 - conserved protein of unknown function, at 4,440,133 to 4,440,342 _3935
Group Condition PP_3931 PP_3932 PP_3933 PP_3934 PP_3935
pH Growth at pH6 and (C) D-Glucose -0.6 +0.3 -0.0 -0.3 -2.0
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.7 -0.3 +0.1 -0.2 -1.5
carbon source ferulate (C); with MOPS +0.0 +0.0 -0.4 +0.3 -2.5
pH Growth at pH9 and (C) Trisodium citrate -0.4 -0.3 -0.6 +0.3 -1.1
stress Isobutyl alcohol 0.6 vol% -0.2 -0.2 -0.0 +0.1 -1.7
nitrogen source Propandiamine (N) -0.4 -0.8 -0.0 -0.4 -0.4
pH Growth at pH6 and (C) Trisodium citrate -0.4 -0.3 +0.0 -0.2 -1.1
nitrogen source NAG (N); with MOPS -0.1 -0.5 +0.3 -0.0 -1.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.7 -0.2 +0.3 -0.4 -0.7
carbon source D-Glucose (C) -0.4 +0.4 +0.2 -0.3 -1.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -0.9 -0.3 -0.5 +0.1 +0.3
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -0.2 +0.2 -0.9 -0.6 +0.2
phage P. putida PUT 7 MOI 1 -0.3 +0.2 -0.9 -0.8 +0.5
nitrogen source Ammonium chloride (N) -0.0 +0.3 -0.4 +0.2 -1.3
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. +0.2 +0.2 -0.1 -0.3 -1.2
carbon source carbon source 2-Deoxy-D-glucose 10 mM -0.3 +0.3 -0.9 +0.4 -0.5
carbon source carbon source Bisabolol 45 mM -0.3 +0.3 -0.3 +0.3 -0.7
carbon source carbon source 4-vinylphenol 0.01 mM -0.4 +0.1 -0.7 +0.5 -0.2
carbon source carbon source Bisabolene 48.9 mM -0.2 +0.4 -0.8 +0.4 -0.5
carbon source carbon source 3-O-methyl-glucose 10 mM -0.5 +0.3 -0.6 +0.5 -0.3
carbon source carbon source 4-vinylphenol 0.1 mM -0.6 +0.4 -0.6 +0.6 -0.3
carbon source ferulate (C); with MOPS +0.3 -0.1 -0.4 +0.4 -0.8
carbon source carbon source D-Glucose 55.5 mM -1.1 +0.2 -0.6 +0.3 +0.8
carbon source carbon source 2-Deoxy-D-glucose 100 mM -0.6 +0.6 -0.6 +0.2 +0.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.5 +0.3 -0.4 -0.0 -0.8
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.2 -0.3 -0.7 +0.3 +0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 +0.7 +0.2 -0.6 +0.1
nitrogen source Propiolactam (N) +0.3 +0.2 +0.6 +0.2 -0.9
phage P. put South MOI 10 +0.3 +0.3 +0.3 -0.1 +1.0
nitrogen source Propiolactam (N) +0.2 +0.2 +0.4 +0.3 +1.3
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