Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3917 and PP_3919 are separated by 39 nucleotidesPP_3919 and PP_3920 are separated by 1 nucleotidesPP_3920 and PP_t61 are separated by 101 nucleotidesPP_t61 and PP_3922 are separated by 154 nucleotides PP_3917: PP_3917 - conserved protein of unknown function, at 4,425,742 to 4,426,080 _3917 PP_3919: PP_3919 - conserved protein of unknown function, at 4,426,120 to 4,426,365 _3919 PP_3920: PP_3920 - putative Phage integrase, at 4,426,367 to 4,427,416 _3920 PP_t61: PP_t61 - tRNA-Cys, at 4,427,518 to 4,427,591 _t61 PP_3922: PP_3922 - putative inner membrane peptidase, at 4,427,746 to 4,428,765 _3922
Group Condition PP_3917 PP_3919 PP_3920 PP_t61 PP_3922
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.3 -2.8 +0.2 N.D. +0.1
temperature Growth at 25C; with MOPS -0.4 -1.8 -0.0 N.D. -0.0
carbon source ferulate (C); with MOPS -0.7 -0.9 -0.6 N.D. +0.1
nitrogen source nitrogen source Ammonium chloride 20mM -0.1 -1.4 -0.2 N.D. -0.3
phage P. putida PUT 7 MOI 1 -0.5 -1.3 +0.2 N.D. -0.3
temperature Growth at 30C; with MOPS -0.1 -1.9 +0.2 N.D. -0.1
nitrogen source DL-2-Aminobutyric (N) -0.4 -0.9 +0.1 N.D. -0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -0.9 +0.1 -0.6 N.D. -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.6 -2.0 +0.1 N.D. -0.3
carbon source L-Lysine (C) -0.3 -1.4 -0.0 N.D. +0.1
carbon source carbon source 2-Deoxy-D-glucose 100 mM -0.7 -1.4 +0.2 N.D. +0.4
nitrogen source nitrogen source nitrite 1mM -0.0 -1.3 +0.3 N.D. -0.5
temperature Growth at 25C +0.4 -2.0 +0.1 N.D. +0.2
carbon source carbon source 3-O-methyl-glucose 10 mM -1.0 -0.5 -0.1 N.D. +0.3
pH pH 9 -0.8 -0.7 +0.1 N.D. +0.2
pH pH 7 -0.7 -1.0 +0.5 N.D. +0.1
carbon source carbon source 3-O-methyl-glucose 50 mM -0.6 -0.8 +0.3 N.D. +0.2
phage P. putida Sci small MOI 0.1 -0.2 -1.5 +0.7 N.D. +0.1
reactor 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. +0.1 -1.5 +0.4 N.D. +0.2
nitrogen source NAG (N); with MOPS +0.9 -1.6 -0.3 N.D. +0.3
pH Growth at pH7 and (C) Trisodium citrate +0.6 -1.0 -0.0 N.D. -0.3
pH pH 8 -0.8 -0.9 +0.5 N.D. +0.5
carbon source carbon source Glycerol 108.6 mM -0.6 -0.7 +0.2 N.D. +0.4
temperature shift Temperature shift 30_to_10; with MOPS -0.7 -0.4 +0.3 N.D. +0.3
carbon source ferulate (C); with MOPS -0.5 -0.6 +0.3 N.D. +0.6
temperature shift Temperature shift 30_to_25; with MOPS +0.4 -0.8 -0.2 N.D. +0.5
nitrogen source 2,6-diaminopimelic (N) -0.1 -0.9 +0.3 N.D. +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.2 +2.0 -0.4 N.D. -0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.2 +1.6 -0.1 N.D. +0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.6 +1.5 +0.1 N.D. +0.3
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