Fitness for 5 genes in Pseudomonas putida KT2440

Top 30 experiments (either direction), sorted by average fitness

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Group	Condition	PP_3880	PP_3881	PP_3882	PP_3883	PP_3884
carbon source	D-Glucose (C); with MOPS	-6.0	+0.1	+0.0	-0.3	-0.1
agar plate interaction	Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant	-0.5	-0.2	-1.4	-3.8	-0.0
temperature	Growth at 30C; with MOPS	-6.0	+0.2	+0.4	+0.9	-0.7
carbon source	ferulate (C); with MOPS	-2.6	-0.1	+0.1	-2.0	+0.2
carbon source	Valerolactone (C)	-3.2	+0.1	-0.1	-0.9	-0.3
soil	Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs	+0.6	-0.0	+0.0	-4.4	-0.2
phage	P. putida Sci small MOI 0.1	-1.5	-0.6	-0.1	-0.8	-0.8
phage	P. putida Sci Big MOI 0.1	-1.5	-0.1	-0.6	-0.5	-0.9
reactor	72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.	-2.2	-0.3	-0.2	-0.7	-0.3
temperature shift	Temperature shift 30_to_25	-1.5	+0.1	-0.3	-0.0	-1.7
stress	3-methyl-1-butanol 0.4 vol%	-2.2	-0.1	-0.5	-0.1	-0.4
carbon source	D-Glucose (C)	-0.2	+0.0	+0.1	-1.7	-1.2
nitrogen source	nitrogen source nitrite 1mM	-2.1	+0.1	+0.3	-0.8	-0.5
reactor	24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v dodecane overlay	-2.0	+0.0	+0.2	-0.5	-0.5
agar plate interaction control	Parafilmed volatile agar plate with no fungus	-2.0	+0.2	-0.4	-0.5	-0.0
pH	Growth at pH7 and (C) Trisodium citrate	-1.5	+0.1	-0.9	+0.6	-0.9
pH	Valerolactone (C) pH 6.5	-1.5	-0.1	+0.2	-1.2	+0.4
pH	Growth at pH7 and (C) D-Glucose	-1.5	+0.0	+0.5	-0.3	-0.8
nitrogen source	Urea (N)	-0.5	-0.1	+0.7	-1.0	-0.9
agar plate interaction	Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant	-1.1	-0.2	-0.4	-0.8	+0.9
nitrogen source	nitrogen source nitrate 5mM	-2.3	+0.1	+0.2	+0.6	-0.2
nitrogen source	nitrate (N)	-1.6	+0.1	+0.5	-0.6	+0.2
nitrogen source	NAG (N); with MOPS	+1.4	+0.0	-0.4	-0.0	-2.2
pH	Growth at pH7 and (C) D-Glucose	+0.4	+0.2	+0.7	-1.4	-0.8
carbon source	4-Aminobutyric 10 mM (C)	-1.8	-0.1	+0.5	+0.7	-0.0
temperature	Growth at 25C; with MOPS	-0.9	+0.1	+0.8	+0.6	-0.9
pH	Growth at pH6 and (C) Trisodium citrate	-1.4	+0.1	+0.7	+0.1	+0.9
carbon source	L-Proline (C)	+0.6	+0.2	-0.1	+1.2	-1.0
agar plate interaction control	Parafilmed volatile agar plate with no fungus	+0.7	-0.0	-0.6	+1.4	+0.6
in planta	Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days	-0.2	+0.4	-0.6	-0.0	+3.0