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Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_3842 and PP_3843 are separated by 125 nucleotides
PP_3843 and PP_3844 are separated by 179 nucleotides
PP_3844 and PP_3845 are separated by 73 nucleotides
PP_3845 and PP_3846 are separated by 140 nucleotides
PP_3842: PP_3842 - conserved protein of unknown function, at 4,365,212 to 4,366,309
_3842
PP_3843: PP_3843 - conserved protein of unknown function, at 4,366,435 to 4,366,734
_3843
PP_3844: PP_3844 - putative D-aminopeptidase, at 4,366,914 to 4,368,047
_3844
PP_3845: PP_3845 - putrescine-binding periplasmic protein, at 4,368,121 to 4,369,230
_3845
PP_3846: PP_3846 - (R)-stereoselective amidase, at 4,369,371 to 4,370,186
_3846
Group
Condition
PP
_3842
PP
_3843
PP
_3844
PP
_3845
PP
_3846
nitrogen source
NAG (N); with MOPS
-0.0
-0.4
-0.3
+0.1
-0.8
carbon source
D-Glucose (C)
-0.3
-0.1
-0.0
+0.1
-0.9
pH
Growth at pH6 and (C) Trisodium citrate
-0.2
-0.5
-0.2
-0.1
+0.0
pH
Growth at pH6 and (C) Trisodium citrate
+0.0
-0.0
-0.1
+0.1
-0.8
temperature
Growth at 30C; with MOPS
-0.0
-0.1
-0.3
+0.1
-0.4
r2a control with 0.2x vogels
R2A control with 0.2X Vogels
-0.1
-0.4
+0.1
-0.1
-0.4
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
-0.3
-0.3
-0.3
+0.1
+0.2
temperature shift
Temperature shift 30_to_25; with MOPS
+0.1
+0.1
-0.4
+0.1
-0.5
stress
Growth with Chloride 200 mM; with MOPS
+0.1
+0.2
-0.3
+0.1
-0.5
pH
Growth at pH7 and (C) D-Glucose
-0.1
+0.2
-0.2
+0.3
-0.3
carbon source
Valerolactone (C)
-0.0
-0.0
+0.2
+0.4
-0.7
temperature
Growth at 25C
-0.0
-0.3
-0.1
-0.2
+0.5
micoeukaryotes
Acanthamoeba sp., muxed culture-2
-0.0
-0.4
+0.2
-0.1
+0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
+0.2
-0.3
-0.1
-0.1
+0.4
carbon source
D-Glucose (C)
+0.0
+0.3
-0.0
+0.2
-0.4
reactor
24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
-0.1
+0.2
-0.3
+0.0
+0.5
pH
Growth at pH6 and (C) Trisodium citrate
+0.1
+0.3
-0.3
-0.1
+0.3
stress
Growth with Chloride 500 mM; with MOPS
+0.0
+0.4
+0.0
+0.3
-0.4
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
+0.2
-0.2
-0.1
+0.1
+0.4
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.3
-0.2
+0.1
+0.0
+0.5
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
+0.0
-0.2
+0.2
+0.2
+0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
+0.4
+0.3
-0.4
+0.1
+0.4
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
+0.2
-0.2
+0.2
+0.1
+0.4
carbon source
D-Glucuronic gamma lactone (C)
+0.1
+0.2
-0.1
+0.3
+0.3
nitrogen source
D-2-Aminobutyric (N)
+0.0
+0.6
+0.1
-0.1
+0.3
phage
P. putida Sci small MOI 0.1
+0.2
-0.2
+0.3
+0.4
+0.6
phage
P. putida Sci Big MOI 0.1
+0.4
+0.3
+0.5
+0.1
+0.2
phage
P. putida Missley MOI 0.1
+0.5
+0.2
+0.4
+0.5
+0.2
phage
P. putida Sci small MOI 0.1
+0.3
+0.3
+0.3
+0.4
+0.7
phage
P. putida PUT 7 MOI 1
+0.5
+0.3
+0.4
+0.3
+0.6
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