Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3842 and PP_3843 are separated by 125 nucleotidesPP_3843 and PP_3844 are separated by 179 nucleotidesPP_3844 and PP_3845 are separated by 73 nucleotidesPP_3845 and PP_3846 are separated by 140 nucleotides PP_3842: PP_3842 - conserved protein of unknown function, at 4,365,212 to 4,366,309 _3842 PP_3843: PP_3843 - conserved protein of unknown function, at 4,366,435 to 4,366,734 _3843 PP_3844: PP_3844 - putative D-aminopeptidase, at 4,366,914 to 4,368,047 _3844 PP_3845: PP_3845 - putrescine-binding periplasmic protein, at 4,368,121 to 4,369,230 _3845 PP_3846: PP_3846 - (R)-stereoselective amidase, at 4,369,371 to 4,370,186 _3846
Group Condition PP_3842 PP_3843 PP_3844 PP_3845 PP_3846
nitrogen source NAG (N); with MOPS -0.0 -0.4 -0.3 +0.1 -0.8
carbon source D-Glucose (C) -0.3 -0.1 -0.0 +0.1 -0.9
pH Growth at pH6 and (C) Trisodium citrate -0.2 -0.5 -0.2 -0.1 +0.0
pH Growth at pH6 and (C) Trisodium citrate +0.0 -0.0 -0.1 +0.1 -0.8
temperature Growth at 30C; with MOPS -0.0 -0.1 -0.3 +0.1 -0.4
r2a control with 0.2x vogels R2A control with 0.2X Vogels -0.1 -0.4 +0.1 -0.1 -0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.3 -0.3 -0.3 +0.1 +0.2
temperature shift Temperature shift 30_to_25; with MOPS +0.1 +0.1 -0.4 +0.1 -0.5
stress Growth with Chloride 200 mM; with MOPS +0.1 +0.2 -0.3 +0.1 -0.5
pH Growth at pH7 and (C) D-Glucose -0.1 +0.2 -0.2 +0.3 -0.3
carbon source Valerolactone (C) -0.0 -0.0 +0.2 +0.4 -0.7
temperature Growth at 25C -0.0 -0.3 -0.1 -0.2 +0.5
micoeukaryotes Acanthamoeba sp., muxed culture-2 -0.0 -0.4 +0.2 -0.1 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.2 -0.3 -0.1 -0.1 +0.4
carbon source D-Glucose (C) +0.0 +0.3 -0.0 +0.2 -0.4
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -0.1 +0.2 -0.3 +0.0 +0.5
pH Growth at pH6 and (C) Trisodium citrate +0.1 +0.3 -0.3 -0.1 +0.3
stress Growth with Chloride 500 mM; with MOPS +0.0 +0.4 +0.0 +0.3 -0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs +0.2 -0.2 -0.1 +0.1 +0.4
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 -0.2 +0.1 +0.0 +0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.0 -0.2 +0.2 +0.2 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.4 +0.3 -0.4 +0.1 +0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.2 -0.2 +0.2 +0.1 +0.4
carbon source D-Glucuronic gamma lactone (C) +0.1 +0.2 -0.1 +0.3 +0.3
nitrogen source D-2-Aminobutyric (N) +0.0 +0.6 +0.1 -0.1 +0.3
phage P. putida Sci small MOI 0.1 +0.2 -0.2 +0.3 +0.4 +0.6
phage P. putida Sci Big MOI 0.1 +0.4 +0.3 +0.5 +0.1 +0.2
phage P. putida Missley MOI 0.1 +0.5 +0.2 +0.4 +0.5 +0.2
phage P. putida Sci small MOI 0.1 +0.3 +0.3 +0.3 +0.4 +0.7
phage P. putida PUT 7 MOI 1 +0.5 +0.3 +0.4 +0.3 +0.6
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