Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3808 and PP_3810 are separated by 133 nucleotidesPP_3810 and PP_3811 are separated by 26 nucleotidesPP_3811 and PP_3812 are separated by 146 nucleotidesPP_3812 and PP_5632 are separated by 141 nucleotides PP_3808: PP_3808 - Antibiotic synthesis protein MbtH, at 4,335,547 to 4,335,768 _3808 PP_3810: PP_3810 - conserved protein of unknown function, at 4,335,902 to 4,336,303 _3810 PP_3811: PP_3811 - Transcriptional regulator, LysR family, at 4,336,330 to 4,337,214 _3811 PP_3812: PP_3812 - putative Lipase, at 4,337,361 to 4,338,380 _3812 PP_5632: PP_5632 - enzyme subunit involved in allo-isoleucine metabolism, at 4,338,522 to 4,338,815 _5632
Group Condition PP_3808 PP_3810 PP_3811 PP_3812 PP_5632
phage PUT 8 MOI 1 -5.0 -0.2 +0.0 -0.1 -0.5
carbon source D-Glucose (C) -4.6 -0.5 +0.4 -0.3 -0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -2.3 -2.7 -0.1 +0.2 -0.2
nitrogen source 2,6-diaminopimelic (N) -2.0 -1.2 -0.5 -0.3 -0.8
pH Growth at pH6 and (C) Trisodium citrate -4.6 +0.2 +0.0 +0.2 -0.3
carbon source Valeric (C) -4.1 +0.7 -0.3 +0.1 -0.3
pH pH 9 -3.2 +0.0 -0.3 -0.2 -0.1
stress Growth with Chloride 500 mM; with MOPS -1.9 -1.7 -0.0 +0.0 -0.0
pH pH 6 -2.8 -0.1 +0.1 -0.1 -0.6
pH Growth at pH9 and (C) Trisodium citrate -2.6 -0.7 +0.1 -0.1 -0.0
pH pH 4 -3.1 -0.1 +0.0 -0.0 +0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -2.1 -0.4 +0.0 -0.5 -0.2
pH Growth at pH6 and (C) Trisodium citrate -0.3 -1.6 -0.0 -0.5 -0.7
carbon source ferulate (C); with MOPS -1.7 -0.7 -0.2 -0.0 -0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs +0.5 -2.8 -0.2 -0.2 -0.3
mixed carbon source mixed (C)s Trisodium citrate 10 mM and L-Meta-tyrosine 10 mM -1.0 -1.2 -0.2 -0.1 -0.3
nitrogen source Imidazole (N) -2.2 -0.4 -0.1 -0.2 +0.2
r2a control with 0.2x vogels R2A control with 0.2X Vogels +0.7 -3.0 -0.3 -0.1 -0.0
carbon source acetate (C) -1.4 -1.2 +0.0 -0.1 +0.1
phage P. put South MOI 10 -1.7 -0.3 -0.3 +0.2 -0.2
carbon source D-Glucose (C) -2.0 +0.7 -0.1 +0.2 -0.3
stress R2A with Polymyxin B sulfate 0.002 mg/ml +1.0 +0.5 -0.6 -1.4 -0.3
carbon source Myristic (C) +1.0 -2.1 -0.1 +0.1 +0.5
pH Valerolactone (C) pH 6.5 -0.5 -0.6 -0.5 -0.5 +1.8
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.2 +1.7 -0.0 +0.2 -0.0
phage P. putida Sci small MOI 0.1 +1.9 -0.9 -0.2 -0.2 -0.0
carbon source Valerolactone (C) +0.2 -0.9 -0.5 +0.0 +1.9
nitrogen source nitrogen source nitrite 1mM +1.3 +0.7 +0.1 -0.6 +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +0.2 +2.3 +0.1 +0.5 +0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.6 +7.4 -0.0 -0.1 -0.6
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