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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_3808 and PP_3810 are separated by 133 nucleotides
PP_3810 and PP_3811 are separated by 26 nucleotides
PP_3811 and PP_3812 are separated by 146 nucleotides
PP_3812 and PP_5632 are separated by 141 nucleotides
PP_3808: PP_3808 - Antibiotic synthesis protein MbtH, at 4,335,547 to 4,335,768
_3808
PP_3810: PP_3810 - conserved protein of unknown function, at 4,335,902 to 4,336,303
_3810
PP_3811: PP_3811 - Transcriptional regulator, LysR family, at 4,336,330 to 4,337,214
_3811
PP_3812: PP_3812 - putative Lipase, at 4,337,361 to 4,338,380
_3812
PP_5632: PP_5632 - enzyme subunit involved in allo-isoleucine metabolism, at 4,338,522 to 4,338,815
_5632
Group
Condition
PP
_3808
PP
_3810
PP
_3811
PP
_3812
PP
_5632
phage
PUT 8 MOI 1
-5.0
-0.2
+0.0
-0.1
-0.5
carbon source
D-Glucose (C)
-4.6
-0.5
+0.4
-0.3
-0.5
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
-2.3
-2.7
-0.1
+0.2
-0.2
nitrogen source
2,6-diaminopimelic (N)
-2.0
-1.2
-0.5
-0.3
-0.8
pH
Growth at pH6 and (C) Trisodium citrate
-4.6
+0.2
+0.0
+0.2
-0.3
carbon source
Valeric (C)
-4.1
+0.7
-0.3
+0.1
-0.3
pH
pH 9
-3.2
+0.0
-0.3
-0.2
-0.1
stress
Growth with Chloride 500 mM; with MOPS
-1.9
-1.7
-0.0
+0.0
-0.0
pH
pH 6
-2.8
-0.1
+0.1
-0.1
-0.6
pH
Growth at pH9 and (C) Trisodium citrate
-2.6
-0.7
+0.1
-0.1
-0.0
pH
pH 4
-3.1
-0.1
+0.0
-0.0
+0.0
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
-2.1
-0.4
+0.0
-0.5
-0.2
pH
Growth at pH6 and (C) Trisodium citrate
-0.3
-1.6
-0.0
-0.5
-0.7
carbon source
ferulate (C); with MOPS
-1.7
-0.7
-0.2
-0.0
-0.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
+0.5
-2.8
-0.2
-0.2
-0.3
mixed carbon source
mixed (C)s Trisodium citrate 10 mM and L-Meta-tyrosine 10 mM
-1.0
-1.2
-0.2
-0.1
-0.3
nitrogen source
Imidazole (N)
-2.2
-0.4
-0.1
-0.2
+0.2
r2a control with 0.2x vogels
R2A control with 0.2X Vogels
+0.7
-3.0
-0.3
-0.1
-0.0
carbon source
acetate (C)
-1.4
-1.2
+0.0
-0.1
+0.1
phage
P. put South MOI 10
-1.7
-0.3
-0.3
+0.2
-0.2
carbon source
D-Glucose (C)
-2.0
+0.7
-0.1
+0.2
-0.3
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
+1.0
+0.5
-0.6
-1.4
-0.3
carbon source
Myristic (C)
+1.0
-2.1
-0.1
+0.1
+0.5
pH
Valerolactone (C) pH 6.5
-0.5
-0.6
-0.5
-0.5
+1.8
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-1.2
+1.7
-0.0
+0.2
-0.0
phage
P. putida Sci small MOI 0.1
+1.9
-0.9
-0.2
-0.2
-0.0
carbon source
Valerolactone (C)
+0.2
-0.9
-0.5
+0.0
+1.9
nitrogen source
nitrogen source nitrite 1mM
+1.3
+0.7
+0.1
-0.6
+0.4
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
+0.2
+2.3
+0.1
+0.5
+0.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.6
+7.4
-0.0
-0.1
-0.6
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