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Protein
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Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_3807 and PP_3808 overlap by 4 nucleotides
PP_3808 and PP_3810 are separated by 133 nucleotides
PP_3810 and PP_3811 are separated by 26 nucleotides
PP_3811 and PP_3812 are separated by 146 nucleotides
PP_3807: PP_3807 - Thioesterase, at 4,334,831 to 4,335,550
_3807
PP_3808: PP_3808 - Antibiotic synthesis protein MbtH, at 4,335,547 to 4,335,768
_3808
PP_3810: PP_3810 - conserved protein of unknown function, at 4,335,902 to 4,336,303
_3810
PP_3811: PP_3811 - Transcriptional regulator, LysR family, at 4,336,330 to 4,337,214
_3811
PP_3812: PP_3812 - putative Lipase, at 4,337,361 to 4,338,380
_3812
Group
Condition
PP
_3807
PP
_3808
PP
_3810
PP
_3811
PP
_3812
phage
PUT 8 MOI 1
-0.3
-5.0
-0.2
+0.0
-0.1
carbon source
D-Glucose (C)
-0.0
-4.6
-0.5
+0.4
-0.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
-0.1
-2.3
-2.7
-0.1
+0.2
pH
Growth at pH6 and (C) Trisodium citrate
+0.1
-4.6
+0.2
+0.0
+0.2
nitrogen source
2,6-diaminopimelic (N)
+0.2
-2.0
-1.2
-0.5
-0.3
stress
Growth with Chloride 500 mM; with MOPS
-0.2
-1.9
-1.7
-0.0
+0.0
carbon source
Valeric (C)
-0.1
-4.1
+0.7
-0.3
+0.1
pH
pH 9
+0.0
-3.2
+0.0
-0.3
-0.2
pH
Growth at pH9 and (C) Trisodium citrate
-0.1
-2.6
-0.7
+0.1
-0.1
pH
pH 4
+0.1
-3.1
-0.1
+0.0
-0.0
nitrogen source
Imidazole (N)
-0.1
-2.2
-0.4
-0.1
-0.2
r2a control with 0.2x vogels
R2A control with 0.2X Vogels
-0.4
+0.7
-3.0
-0.3
-0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
-0.2
-2.1
-0.4
+0.0
-0.5
temperature shift
Temperature shift 10_to_34; with MOPS
-0.1
-1.8
-0.8
-0.1
-0.0
carbon source
acetate (C)
+0.0
-1.4
-1.2
+0.0
-0.1
pH
pH 6
+0.2
-2.8
-0.1
+0.1
-0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
+0.1
+0.5
-2.8
-0.2
-0.2
phage
P. put South MOI 10
-0.2
-1.7
-0.3
-0.3
+0.2
carbon source
ferulate (C); with MOPS
+0.6
-1.7
-0.7
-0.2
-0.0
carbon source
D-Glucose (C)
-0.3
-2.0
+0.7
-0.1
+0.2
carbon source
Myristic (C)
+0.2
+1.0
-2.1
-0.1
+0.1
nitrogen source
D-2-Aminobutyric (N)
-0.7
-1.1
+1.2
-0.2
-0.0
pH
Growth at pH7 and (C) D-Glucose
-0.5
+0.9
-1.1
+0.2
+0.1
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
+0.3
+1.0
+0.5
-0.6
-1.4
phage
P. putida Sci small MOI 0.1
-0.1
+1.9
-0.9
-0.2
-0.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.3
-1.2
+1.7
-0.0
+0.2
nitrogen source
nitrogen source nitrite 1mM
-0.1
+1.3
+0.7
+0.1
-0.6
micoeukaryotes
Tetrahymena sp., Mixed culture-2
+1.1
+0.4
+1.1
-0.1
+0.2
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
+0.0
+0.2
+2.3
+0.1
+0.5
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.3
+0.6
+7.4
-0.0
-0.1
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