Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3807 and PP_3808 overlap by 4 nucleotidesPP_3808 and PP_3810 are separated by 133 nucleotidesPP_3810 and PP_3811 are separated by 26 nucleotidesPP_3811 and PP_3812 are separated by 146 nucleotides PP_3807: PP_3807 - Thioesterase, at 4,334,831 to 4,335,550 _3807 PP_3808: PP_3808 - Antibiotic synthesis protein MbtH, at 4,335,547 to 4,335,768 _3808 PP_3810: PP_3810 - conserved protein of unknown function, at 4,335,902 to 4,336,303 _3810 PP_3811: PP_3811 - Transcriptional regulator, LysR family, at 4,336,330 to 4,337,214 _3811 PP_3812: PP_3812 - putative Lipase, at 4,337,361 to 4,338,380 _3812
Group Condition PP_3807 PP_3808 PP_3810 PP_3811 PP_3812
phage PUT 8 MOI 1 -0.3 -5.0 -0.2 +0.0 -0.1
carbon source D-Glucose (C) -0.0 -4.6 -0.5 +0.4 -0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -0.1 -2.3 -2.7 -0.1 +0.2
pH Growth at pH6 and (C) Trisodium citrate +0.1 -4.6 +0.2 +0.0 +0.2
nitrogen source 2,6-diaminopimelic (N) +0.2 -2.0 -1.2 -0.5 -0.3
stress Growth with Chloride 500 mM; with MOPS -0.2 -1.9 -1.7 -0.0 +0.0
carbon source Valeric (C) -0.1 -4.1 +0.7 -0.3 +0.1
pH pH 9 +0.0 -3.2 +0.0 -0.3 -0.2
pH Growth at pH9 and (C) Trisodium citrate -0.1 -2.6 -0.7 +0.1 -0.1
pH pH 4 +0.1 -3.1 -0.1 +0.0 -0.0
nitrogen source Imidazole (N) -0.1 -2.2 -0.4 -0.1 -0.2
r2a control with 0.2x vogels R2A control with 0.2X Vogels -0.4 +0.7 -3.0 -0.3 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.2 -2.1 -0.4 +0.0 -0.5
temperature shift Temperature shift 10_to_34; with MOPS -0.1 -1.8 -0.8 -0.1 -0.0
carbon source acetate (C) +0.0 -1.4 -1.2 +0.0 -0.1
pH pH 6 +0.2 -2.8 -0.1 +0.1 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs +0.1 +0.5 -2.8 -0.2 -0.2
phage P. put South MOI 10 -0.2 -1.7 -0.3 -0.3 +0.2
carbon source ferulate (C); with MOPS +0.6 -1.7 -0.7 -0.2 -0.0
carbon source D-Glucose (C) -0.3 -2.0 +0.7 -0.1 +0.2
carbon source Myristic (C) +0.2 +1.0 -2.1 -0.1 +0.1
nitrogen source D-2-Aminobutyric (N) -0.7 -1.1 +1.2 -0.2 -0.0
pH Growth at pH7 and (C) D-Glucose -0.5 +0.9 -1.1 +0.2 +0.1
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.3 +1.0 +0.5 -0.6 -1.4
phage P. putida Sci small MOI 0.1 -0.1 +1.9 -0.9 -0.2 -0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 -1.2 +1.7 -0.0 +0.2
nitrogen source nitrogen source nitrite 1mM -0.1 +1.3 +0.7 +0.1 -0.6
micoeukaryotes Tetrahymena sp., Mixed culture-2 +1.1 +0.4 +1.1 -0.1 +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +0.0 +0.2 +2.3 +0.1 +0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 +0.6 +7.4 -0.0 -0.1
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