Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3802 and PP_3803 overlap by 1 nucleotidesPP_3803 and PP_3804 overlap by 4 nucleotidesPP_3804 and PP_3805 are separated by 83 nucleotidesPP_3805 and PP_3806 are separated by 26 nucleotides PP_3802: PP_3802 - putative Cation ABC transporter, ATP-binding protein, at 4,330,555 to 4,331,301 _3802 PP_3803: PP_3803 - putative Cation ABC transporter, permease protein, at 4,331,301 to 4,332,200 _3803 PP_3804: PP_3804 - conserved exported protein of unknown function, at 4,332,197 to 4,333,060 _3804 PP_3805: PP_3805 - lipoprotein required for swarming, at 4,333,144 to 4,334,196 _3805 PP_3806: PP_3806 - Hydrolase, isochorismatase family, at 4,334,223 to 4,334,774 _3806
Group Condition PP_3802 PP_3803 PP_3804 PP_3805 PP_3806
nitrogen source D-2-Aminobutyric (N) -0.6 -0.2 +0.1 -0.7 -3.8
carbon source 3-Hydroxyhexanoic (C) -0.0 +0.4 -0.1 -0.0 -4.7
pH Growth at pH7 and (C) D-Glucose -1.0 -1.0 -2.2 +0.1 -0.2
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -0.5 -0.5 -1.7 -0.2 -0.6
pH pH 9 -1.2 -1.1 -1.1 +0.1 -0.2
carbon source D-Glucose (C) +0.0 +0.6 -1.5 +0.2 -2.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -1.5 -1.0 -0.6 +0.0 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -1.0 -1.2 -0.6 -0.0 -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 -1.7 -0.9 +0.3 -0.6
pH Valerolactone (C) pH 6.5 -0.2 -0.9 -0.1 +0.3 -2.0
phage P. putida PUT 7 MOI 1 -1.1 -0.1 -0.7 -0.5 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.5 -1.0 +0.2 +0.1 -1.5
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.8 -0.3 -0.2 -0.0 -1.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.8 -0.8 -0.9 +0.1 -0.4
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.6 -0.5 -0.4 -0.7 -0.5
reactor 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -0.8 -0.9 -0.9 +0.1 -0.1
carbon source 3-Hydroxyhexanoic (C) +0.2 +0.4 -0.1 -0.0 -3.0
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.0 -0.9 -1.1 +0.1 +0.5
carbon source acetate (C) -0.5 +0.4 -1.1 +0.0 -1.0
pH D-Glucose (C) pH 7 +0.3 -0.8 +0.2 +0.1 -2.0
phage P. putida Sci small MOI 0.1 -0.7 +0.3 -0.1 -0.6 -1.1
phage P. putida Missley MOI 0.1 -0.6 +0.3 -0.8 -0.1 -0.9
pH Growth at pH8 and (C) D-Glucose +0.1 -0.1 +0.3 -0.5 -1.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.7 -1.1 -0.6 +0.1 +0.4
nitrogen source Propiolactam (N) +0.6 +0.1 +0.1 -0.1 -2.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -1.0 -0.9 -0.2 +0.5 +0.1
nitrogen source nitrogen source nitrate 5mM -0.7 +0.7 -0.6 +0.1 -0.6
nitrogen source D-2-Aminobutyric (N) +0.8 -0.3 +0.6 +0.2 -2.4
carbon source ferulate (C); with MOPS +0.7 -0.1 +0.2 -0.4 -1.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.5 +0.8 -0.0 -0.4 +1.1
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