Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_3763 and PP_3764 are separated by 67 nucleotidesPP_3764 and PP_3765 are separated by 349 nucleotidesPP_3765 and PP_3766 are separated by 224 nucleotidesPP_3766 and PP_3767 are separated by 97 nucleotides PP_3763: PP_3763 - putative CobF protein, at 4,293,254 to 4,294,003 _3763 PP_3764: PP_3764 - outer-membrane porin D, at 4,294,071 to 4,295,372 _3764 PP_3765: PP_3765 - H-NS family protein MvaT, at 4,295,722 to 4,296,081 _3765 PP_3766: PP_3766 - Lactoylglutathione lyase, at 4,296,306 to 4,296,833 _3766 PP_3767: PP_3767 - conserved protein of unknown function, at 4,296,931 to 4,297,173 _3767
Group Condition PP_3763 PP_3764 PP_3765 PP_3766 PP_3767
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs +0.3 -0.0 -0.7 -5.2 -0.1
pH Growth at pH6 and (C) D-Glucose -0.0 -0.0 +0.7 -6.3 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.6 -0.0 -0.7 -2.8 -0.0
carbon source Myristic (C) -0.5 -0.0 -0.2 -2.3 -0.0
carbon source D-Glucose (C) +0.4 -0.0 -1.4 -1.4 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.9 -0.1 -0.6 -1.3 +0.2
stress Growth with Chloride 500 mM; with MOPS -0.5 +0.2 +0.0 -2.2 -0.1
carbon source Valerolactone (C) -0.6 +0.1 -0.4 -0.9 -0.7
micoeukaryotes Tetrahymena sp., Mixed culture-2 +0.4 +0.2 -2.2 -0.9 +0.1
carbon source p-Coumaric (C) +0.2 +0.0 +0.1 -2.5 +0.0
phage P. putida Sci small MOI 0.1 -2.5 -0.0 +0.4 -0.4 +0.4
carbon source ferulate (C); with MOPS -1.3 +0.0 +0.3 -0.6 -0.5
pH Valerolactone (C) pH 6.5 +0.3 -0.2 -0.3 -1.1 -0.8
carbon source Tween 20 (C) +0.0 -0.2 +0.5 -1.6 -0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -1.5 +0.2 +0.7 -0.3 -0.6
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.0 -0.0 -1.1 -0.9 +0.5
carbon source carbon source 4-vinylphenol 0.1 mM +0.4 +0.2 +0.3 -1.5 -0.8
carbon source carbon source D-Glucose 55.5 mM +0.2 +0.2 +0.4 -1.8 -0.1
phage PUT8 MOI 0.1 +0.1 -0.1 +0.7 -1.5 -0.3
nitrogen source Caprolactam (N) +0.2 +0.1 +0.2 -1.8 +0.4
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -0.5 +0.2 -1.3 +0.5 +0.1
pH pH 8 +0.5 +0.2 +0.6 -1.9 -0.1
carbon source carbon source D-Xylose 66.6 mM and Glycerol 108.6 mM +0.4 +0.2 +0.6 -1.4 -0.3
pH pH 5 +0.7 +0.2 +0.2 -1.2 -0.4
temperature Growth at 30C; with MOPS +0.5 -0.4 -0.3 +0.8 -0.9
carbon source carbon source 2-Deoxy-D-glucose 50 mM +0.4 -0.2 +1.0 -0.8 -0.6
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.4 +0.3 -0.7 -0.3 -0.7
carbon source ferulate (C); with MOPS +0.1 +0.3 +1.3 -1.4 +0.3
nitrogen source Urea (N) -0.7 -0.1 +1.0 +1.1 +0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 +0.3 -0.4 +1.3 +1.0
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